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Literature summary extracted from

  • Matsushika, A.; Watanabe, S.; Kodaki, T.; Makino, K.; Sawayama, S.
    Bioethanol production from xylose by recombinant Saccharomyces cerevisiae expressing xylose reductase, NADP(+)-dependent xylitol dehydrogenase, and xylulokinase (2008), J. Biosci. Bioeng., 105, 296-299.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.10 a recombinant Saccharomyces cerevisiae strain D-XR/ARSdR/XK, in which protein engineered NADP+-dependent XDH is expressed, shows 40% increased ethanol production and 23% decrease in xylitol excretion as compared with the reference strain D-XR/XDH/XK expressing the wild-type XDH Scheffersomyces stipitis
2.7.1.17 expressed in Saccharomyces cerevisiae strain D452-2, DNA from Saccharomyces cerevisiae strain S288C, strain D-XR/ARSdR with naturally occuring XK-activity Saccharomyces cerevisiae
2.7.1.17 expressed in Saccharomyces cerevisiae strain D452-2, DNA from Saccharomyces cerevisiae strain S288C, strain D-XR/ARSdR/XK with upegulated XK-activity, entire coding sequence of XK with PGK promoter and terminator inserted into pAUR101 shuttle vector (Takara Bio, Kyoto) integrating in Saccharomyces cerevisiae strain D452-2 Saccharomyces cerevisiae
2.7.1.17 expressed in Saccharomyces cerevisiae strain D452-2, DNA from Saccharomyces cerevisiae strain S288C, strain D-XR/XDH with naturally occuring enzyme-activity Saccharomyces cerevisiae
2.7.1.17 expressed in Saccharomyces cerevisiae strain D452-2, DNA from Saccharomyces cerevisiae strain S288C, strain D-XR/XDH/XK with upegulated XK-activity, entire coding sequence of XK with PGK promoter and terminator inserted into pAUR101 shuttle vector integrating in Saccharomyces cerevisiae strain D452-2 Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.10 Scheffersomyces stipitis
-
-
-
2.7.1.17 Saccharomyces cerevisiae
-
-
-
2.7.1.17 Saccharomyces cerevisiae D452-2 D-XR/ARSdR
-
-
-
2.7.1.17 Saccharomyces cerevisiae D452-2 D-XR/ARSdR/XK
-
-
-
2.7.1.17 Saccharomyces cerevisiae D452-2 D-XR/XDH
-
-
-
2.7.1.17 Saccharomyces cerevisiae D452-2 D-XR/XDH/XK
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.17 additional information
-
 0.034 +/-0.002 U/mg, more than 2fold higher values than D-XR/XDH and D-XR/ARSdR, XK reaction forming ADP coupled with pyruvate kinase (PK) and lactate dehydrogenase (LDH) reactions, activity is measured by monitoring the oxidation of NADH at 340 nm in reaction mixture, one unit of enzyme activity is defined as the amount of enzyme that reduces or oxidizes 1 micromol NAD(P)+ or NAD(P)H per minute. Saccharomyces cerevisiae
2.7.1.17 additional information
-
 strain D-XR/ARSdR/XK, 0.030 +/-0.002 U/mg, more than two-fold higher values than D-XR/XDH and D-XR/ARSdR, reaction forming ADP coupled with pyruvate kinase (PK) and lactate dehydrogenase (LDH) reactions, activity is measured by monitoring the oxidation of NADH at 340 nm in reaction mixture, one unit of enzyme activity is defined as the amount of enzyme that reduces or oxidizes 1 micromol NAD(P)+ or NAD(P)H per minute. Saccharomyces cerevisiae
2.7.1.17 additional information
-
 strain D452-2 D-XR/ARSdR, 0.013 +/-0.001 U/mg, reaction forming ADP coupled with pyruvate kinase (PK) and lactate dehydrogenase (LDH) reactions, activity is measured by monitoring the oxidation of NADH at 340 nm in reaction mixture, one unit of enzyme activity is defined as the amount of enzyme that reduces or oxidizes 1 micromol NAD(P)+ or NAD(P)H per minute. Saccharomyces cerevisiae
2.7.1.17 additional information
-
 strain D452-2 D-XR/XDH, 0.014 +/-0.002 U/mg, reaction forming ADP coupled with pyruvate kinase (PK) and lactate dehydrogenase (LDH) reactions, activity is measured by monitoring the oxidation of NADH at 340 nm in reaction mixture, one unit of enzyme activity is defined as the amount of enzyme that reduces or oxidizes 1 micromol NAD(P)+ or NAD(P)H per minute. Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.17 ATP + D-xylulose
-
 Saccharomyces cerevisiae ADP + D-xylulose 5-phosphate
-
 r
2.7.1.17 ATP + D-xylulose
-
 Saccharomyces cerevisiae D452-2 D-XR/XDH/XK ADP + D-xylulose 5-phosphate
-
 r
2.7.1.17 ATP + D-xylulose
-
 Saccharomyces cerevisiae D452-2 D-XR/ARSdR/XK ADP + D-xylulose 5-phosphate
-
 r
2.7.1.17 ATP + D-xylulose
-
 Saccharomyces cerevisiae D452-2 D-XR/XDH ADP + D-xylulose 5-phosphate
-
 r
2.7.1.17 ATP + D-xylulose
-
 Saccharomyces cerevisiae D452-2 D-XR/ARSdR ADP + D-xylulose 5-phosphate
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.1.1.10 NADP(+)-dependent xylitol dehydrogenase
-
 Scheffersomyces stipitis
1.1.1.10 XDH
-
 Scheffersomyces stipitis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.17 30
-
-
 Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.17 ATP
-
 Saccharomyces cerevisiae