BRENDA - Enzyme Database

Eukaryotic and bacterial gene clusters related to an alternative pathway of non-phosphorylated L-rhamnose metabolism

Watanabe, S.; Saimura, M.; Makino, K.; J. Biol. Chem. 283, 20372-20382 (2008)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Cloned (Commentary)
Organism
1.1.1.173
gene LRA1, DNA and amino acid sequence determination and analysis, genetic organization, functional expression of His-tagged enzyme in Escherichia coli
Debaryomyces hansenii
1.1.1.173
gene LRA1, DNA and amino acid sequence determination and analysis, genetic organization, functional expression of His-tagged enzyme in Escherichia coli
Scheffersomyces stipitis
1.1.1.378
expression in Escherichia coli
Azotobacter vinelandii
3.1.1.65
overexpression in Escherichia coli DH5 alpha as His6-tagged enzyme
Azotobacter vinelandii
3.1.1.65
overexpression in Escherichia coli DH5 alpha as His6-tagged enzyme
Debaryomyces hansenii
3.1.1.65
overexpression in Escherichia coli DH5 alpha as His6-tagged enzyme
Scheffersomyces stipitis
4.1.2.53
expressed in Escherichia coli DH5alpha cells
Azotobacter vinelandii
4.1.2.53
expressed in Escherichia coli DH5alpha cells
Scheffersomyces stipitis
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.2.53
Ca2+
complete inhibition in the presence of Ca2+
Azotobacter vinelandii
4.1.2.53
Co2+
about 75% residual activity in the presence of Co2+
Azotobacter vinelandii
4.1.2.53
Cu2+
complete inhibition in the presence of Cu2+
Azotobacter vinelandii
4.1.2.53
Fe2+
complete inhibition in the presence of Fe2+
Azotobacter vinelandii
4.1.2.53
Mg2+
about 25% residual activity in the presence of Mg2+
Azotobacter vinelandii
4.1.2.53
Ni2+
about 10% residual activity in the presence of Ni2+
Azotobacter vinelandii
4.1.2.53
Zn2+
about 80% residual activity in the presence of Zn2+
Azotobacter vinelandii
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.173
1.71
-
L-rhamnose
pH 9.0, 30C, recombinant enzyme
Scheffersomyces stipitis
1.1.1.173
9.35
-
L-rhamnose
pH 9.0, 30C, recombinant enzyme
Debaryomyces hansenii
1.1.1.378
2.34
-
L-rhamnose
pH 9.0, 30C, cosubstrate: NADP+
Azotobacter vinelandii
1.1.1.378
2.61
-
L-rhamnose
pH 9.0, 30C, cosubstrate: NAD+
Azotobacter vinelandii
1.1.1.378
4.96
-
L-Lyxose
pH 9.0, 30C, cosubstrate: NADP+
Azotobacter vinelandii
1.1.1.378
8.43
-
L-Lyxose
pH 9.0, 30C, cosubstrate: NAD+
Azotobacter vinelandii
1.1.1.378
89.3
-
L-Mannose
pH 9.0, 30C, cosubstrate: NADP+
Azotobacter vinelandii
1.1.1.378
108
-
L-Mannose
pH 9.0, 30C, cosubstrate: NAD+
Azotobacter vinelandii
1.1.1.378
582
-
L-fucose
pH 9.0, 30C, cosubstrate: NADP+
Azotobacter vinelandii
1.1.1.378
673
-
L-fucose
pH 9.0, 30C, cosubstrate: NAD+
Azotobacter vinelandii
4.1.2.53
0.915
-
2-dehydro-3-deoxy-L-rhamnonate
in 50 mM sodium phosphate (pH 7.0), 5 mM Mn2+, temperature not specified in the publication
Azotobacter vinelandii
4.1.2.53
1.37
-
2-dehydro-3-deoxy-L-rhamnonate
in 50 mM sodium phosphate (pH 7.0), 5 mM Mn2+, temperature not specified in the publication
Scheffersomyces stipitis
4.2.1.90
0.115
-
L-Rhamnonate
pH 7.0, 30C
Azotobacter vinelandii
4.2.1.90
1.37
-
L-Rhamnonate
pH 7.0, 30C
Scheffersomyces stipitis
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.1.2.53
Co2+
about 240% activity in the presence of Co2+
Scheffersomyces stipitis
4.1.2.53
Mg2+
about 150% activity in the presence of Co2+
Scheffersomyces stipitis
4.1.2.53
Mg2+
more than 250% activity in the presence of Mg2+, 5 mM MgCl2 used in assay conditions
Scheffersomyces stipitis
4.1.2.53
additional information
not stimulated by Mg2+, Co2+, Ni2+, Zn2+, Fe2+, Cu2+, and Ca2+
Azotobacter vinelandii
4.1.2.53
additional information
not stimulated by Zn2+, Fe2+, Cu2+, and Ca2+
Scheffersomyces stipitis
4.1.2.53
Ni2+
about 140% activity in the presence of Ni2+
Scheffersomyces stipitis
4.2.1.90
Mg2+
-
Azotobacter vinelandii
4.2.1.90
Mg2+
-
Debaryomyces hansenii
4.2.1.90
Mg2+
-
Scheffersomyces stipitis
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.173
25000
-
x * 25000, SDS-PAGE
Scheffersomyces stipitis
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.1.1.173
L-rhamnose + NAD+
Debaryomyces hansenii
-
L-rhamno-1,4-lactone + NADH
-
-
r
1.1.1.173
L-rhamnose + NAD+
Scheffersomyces stipitis
-
L-rhamno-1,4-lactone + NADH
-
-
r
1.1.1.173
L-rhamnose + NAD+
Debaryomyces hansenii NBRC0083
-
L-rhamno-1,4-lactone + NADH
-
-
r
1.1.1.378
L-rhamnose + NAD(P)+
Azotobacter vinelandii
first enzyme of the non-phosphorylative L-rhamnose pathway in archaea, that is analogous to the Entner-Doudoroff pathway
L-rhamno-1,4-lactone + NAD(P)H + H+
-
-
?
3.1.1.65
L-rhamnono-1,4-lactone + H2O
Azotobacter vinelandii
-
L-rhamnonate
-
-
?
3.1.1.65
L-rhamnono-1,4-lactone + H2O
Debaryomyces hansenii
-
L-rhamnonate
-
-
?
3.1.1.65
L-rhamnono-1,4-lactone + H2O
Scheffersomyces stipitis
-
L-rhamnonate
-
-
?
3.1.1.65
L-rhamnono-1,4-lactone + H2O
Debaryomyces hansenii NBRC0083
-
L-rhamnonate
-
-
?
3.1.1.65
L-rhamnono-1,4-lactone + H2O
Azotobacter vinelandii NBRC 102612
-
L-rhamnonate
-
-
?
4.1.2.53
2-dehydro-3-deoxy-L-rhamnonate
Azotobacter vinelandii
preferred substrate
pyruvate + (R)-lactaldehyde
-
-
?
4.1.2.53
2-dehydro-3-deoxy-L-rhamnonate
Scheffersomyces stipitis
preferred substrate
pyruvate + (R)-lactaldehyde
-
-
?
4.2.1.90
L-rhamnonate
Azotobacter vinelandii
step in the alternative pathway for L-rhamnose metabolism, overview
2-dehydro-3-deoxy-L-rhamnonate + H2O
-
-
?
4.2.1.90
L-rhamnonate
Debaryomyces hansenii
step in the alternative pathway for L-rhamnose metabolism, overview
2-dehydro-3-deoxy-L-rhamnonate + H2O
-
-
?
4.2.1.90
L-rhamnonate
Scheffersomyces stipitis
step in the alternative pathway for L-rhamnose metabolism, overview
2-dehydro-3-deoxy-L-rhamnonate + H2O
-
-
?
4.2.1.90
L-rhamnonate
Debaryomyces hansenii NBRC0083
step in the alternative pathway for L-rhamnose metabolism, overview
2-dehydro-3-deoxy-L-rhamnonate + H2O
-
-
?
4.2.1.90
L-rhamnonate
Azotobacter vinelandii NBRC 102612
step in the alternative pathway for L-rhamnose metabolism, overview
2-dehydro-3-deoxy-L-rhamnonate + H2O
-
-
?
Organism
EC Number
Organism
UniProt
Commentary
Textmining
1.1.1.173
Debaryomyces hansenii
-
gene LRA1
-
1.1.1.173
Debaryomyces hansenii NBRC0083
-
gene LRA1
-
1.1.1.173
Scheffersomyces stipitis
-
gene LRA1
-
1.1.1.378
Azotobacter vinelandii
-
-
-
1.1.1.378
Azotobacter vinelandii NBRC 102612
-
-
-
3.1.1.65
Azotobacter vinelandii
-
-
-
3.1.1.65
Azotobacter vinelandii NBRC 102612
-
-
-
3.1.1.65
Debaryomyces hansenii
-
-
-
3.1.1.65
Debaryomyces hansenii NBRC0083
-
-
-
3.1.1.65
Scheffersomyces stipitis
A3LZU8
-
-
4.1.2.53
Azotobacter vinelandii
-
-
-
4.1.2.53
Scheffersomyces stipitis
-
-
-
4.2.1.90
Azotobacter vinelandii
-
-
-
4.2.1.90
Azotobacter vinelandii NBRC 102612
-
-
-
4.2.1.90
Debaryomyces hansenii
-
-
-
4.2.1.90
Debaryomyces hansenii NBRC0083
-
-
-
4.2.1.90
Scheffersomyces stipitis
-
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
1.1.1.173
native enzyme 42.8fold by anion exchange chromatography, ultrafiltration, gel filtration, hydroxyapatite chromatography, and again gel filtration, recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography
Scheffersomyces stipitis
1.1.1.173
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography
Debaryomyces hansenii
1.1.1.378
-
Azotobacter vinelandii
3.1.1.65
nickel-nitrilotriacetic acid superflow column
Azotobacter vinelandii
3.1.1.65
nickel-nitrilotriacetic acid superflow column
Debaryomyces hansenii
3.1.1.65
nickel-nitrilotriacetic acid superflow column
Scheffersomyces stipitis
4.1.2.53
Ni-NTA column chromatography
Azotobacter vinelandii
4.1.2.53
Ni-NTA column chromatography
Scheffersomyces stipitis
Reaction
EC Number
Reaction
Commentary
Organism
Reaction ID
1.1.1.173
L-rhamnofuranose + NAD+ = L-rhamno-1,4-lactone + NADH + H+
the catalytic triad is formed by conserved Ser-Tyr-Lys residues
Debaryomyces hansenii
1.1.1.173
L-rhamnofuranose + NAD+ = L-rhamno-1,4-lactone + NADH + H+
the catalytic triad is formed by conserved Ser148-Tyr161-Lys165 residues
Scheffersomyces stipitis
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
1.1.1.173
29.9
-
purified native enzyme
Scheffersomyces stipitis
1.1.1.173
50.4
-
purified recombinant enzyme
Scheffersomyces stipitis
1.1.1.173
53.3
-
purified recombinant enzyme
Debaryomyces hansenii
1.1.1.378
0.15
-
pH 9.0, 30C, substrates: L-fucose, NAD+
Azotobacter vinelandii
1.1.1.378
0.27
-
pH 9.0, 30C, substrates: L-fucose, NADP+
Azotobacter vinelandii
1.1.1.378
1.69
-
pH 9.0, 30C, substrates: L-mannose, NAD+
Azotobacter vinelandii
1.1.1.378
3.64
-
pH 9.0, 30C, substrates: L-mannose, NADP+
Azotobacter vinelandii
1.1.1.378
50.7
-
pH 9.0, 30C, substrates: L-lyxose, NAD+
Azotobacter vinelandii
1.1.1.378
72.1
-
pH 9.0, 30C, substrates: L-rhamnose, NAD+
Azotobacter vinelandii
1.1.1.378
125
-
pH 9.0, 30C, substrates: L-lyxose, NADP+
Azotobacter vinelandii
1.1.1.378
163
-
pH 9.0, 30C, substrates: L-rhamnose, NADP+
Azotobacter vinelandii
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1.1.1.173
L-rhamnose + NAD+
-
687737
Debaryomyces hansenii
L-rhamno-1,4-lactone + NADH
-
-
-
r
1.1.1.173
L-rhamnose + NAD+
-
687737
Scheffersomyces stipitis
L-rhamno-1,4-lactone + NADH
-
-
-
r
1.1.1.173
L-rhamnose + NAD+
-
687737
Debaryomyces hansenii NBRC0083
L-rhamno-1,4-lactone + NADH
-
-
-
r
1.1.1.378
L-fucose + NAD+
the enzyme shows significant preference for NADP+ over NAD+
687737
Azotobacter vinelandii
?
-
-
-
?
1.1.1.378
L-fucose + NADP+
the enzyme shows significant preference for NADP+ over NAD+
687737
Azotobacter vinelandii
?
-
-
-
?
1.1.1.378
L-lyxose + NAD+
the enzyme shows significant preference for NADP+ over NAD+
687737
Azotobacter vinelandii
?
-
-
-
?
1.1.1.378
L-lyxose + NADP+
the enzyme shows significant preference for NADP+ over NAD+
687737
Azotobacter vinelandii
?
-
-
-
?
1.1.1.378
L-mannose + NAD+
the enzyme shows significant preference for NADP+ over NAD+
687737
Azotobacter vinelandii
?
-
-
-
?
1.1.1.378
L-mannose + NADP+
the enzyme shows significant preference for NADP+ over NAD+
687737
Azotobacter vinelandii
?
-
-
-
?
1.1.1.378
L-rhamnose + NAD(P)+
first enzyme of the non-phosphorylative L-rhamnose pathway in archaea, that is analogous to the Entner-Doudoroff pathway
687737
Azotobacter vinelandii
L-rhamno-1,4-lactone + NAD(P)H + H+
-
-
-
?
1.1.1.378
L-rhamnose + NAD+
the enzyme shows significant preference for NADP+ over NAD+
687737
Azotobacter vinelandii
L-rhamno-1,4-lactone + NADH + H+
-
-
-
?
1.1.1.378
L-rhamnose + NADP+
the enzyme shows significant preference for NADP+ over NAD+
687737
Azotobacter vinelandii
L-rhamno-1,4-lactone + NADPH + H+
-
-
-
?
3.1.1.65
L-rhamnono-1,4-lactone + H2O
-
687737
Azotobacter vinelandii
L-rhamnonate
-
-
-
?
3.1.1.65
L-rhamnono-1,4-lactone + H2O
-
687737
Debaryomyces hansenii
L-rhamnonate
-
-
-
?
3.1.1.65
L-rhamnono-1,4-lactone + H2O
-
687737
Scheffersomyces stipitis
L-rhamnonate
-
-
-
?
3.1.1.65
L-rhamnono-1,4-lactone + H2O
-
687737
Debaryomyces hansenii NBRC0083
L-rhamnonate
-
-
-
?
3.1.1.65
L-rhamnono-1,4-lactone + H2O
-
687737
Azotobacter vinelandii NBRC 102612
L-rhamnonate
-
-
-
?
4.1.2.53
2-dehydro-3-deoxy-L-mannonate
13.3% activity compared to 2-dehydro-3-deoxy-L-rhamnonate
687737
Scheffersomyces stipitis
?
-
-
-
?
4.1.2.53
2-dehydro-3-deoxy-L-mannonate
6.7% activity compared to 2-dehydro-3-deoxy-L-rhamnonate
687737
Azotobacter vinelandii
?
-
-
-
?
4.1.2.53
2-dehydro-3-deoxy-L-rhamnonate
preferred substrate
687737
Azotobacter vinelandii
pyruvate + (R)-lactaldehyde
-
-
-
?
4.1.2.53
2-dehydro-3-deoxy-L-rhamnonate
preferred substrate
687737
Scheffersomyces stipitis
pyruvate + (R)-lactaldehyde
-
-
-
?
4.1.2.53
2-dehydro-3-deoxy-L-xylonate
54.7% activity compared to 2-dehydro-3-deoxy-L-rhamnonate
687737
Scheffersomyces stipitis
?
-
-
-
?
4.1.2.53
2-dehydro-3-deoxy-L-xylonate
7.6% activity compared to 2-dehydro-3-deoxy-L-rhamnonate
687737
Azotobacter vinelandii
?
-
-
-
?
4.2.1.90
L-lyxonate
-
687737
Azotobacter vinelandii
2-dehydro-3-deoxy-L-lyxonate + H2O
-
-
-
?
4.2.1.90
L-lyxonate
-
687737
Azotobacter vinelandii NBRC 102612
2-dehydro-3-deoxy-L-lyxonate + H2O
-
-
-
?
4.2.1.90
L-mannonate
-
687737
Azotobacter vinelandii
2-dehydro-3-deoxy-L-mannonate + H2O
-
-
-
?
4.2.1.90
L-mannonate
-
687737
Azotobacter vinelandii NBRC 102612
2-dehydro-3-deoxy-L-mannonate + H2O
-
-
-
?
4.2.1.90
L-rhamnonate
-
687737
Debaryomyces hansenii
2-dehydro-3-deoxy-L-rhamnonate + H2O
-
-
-
?
4.2.1.90
L-rhamnonate
-
687737
Scheffersomyces stipitis
2-dehydro-3-deoxy-L-rhamnonate + H2O
-
-
-
?
4.2.1.90
L-rhamnonate
step in the alternative pathway for L-rhamnose metabolism, overview
687737
Azotobacter vinelandii
2-dehydro-3-deoxy-L-rhamnonate + H2O
-
-
-
?
4.2.1.90
L-rhamnonate
step in the alternative pathway for L-rhamnose metabolism, overview
687737
Debaryomyces hansenii
2-dehydro-3-deoxy-L-rhamnonate + H2O
-
-
-
?
4.2.1.90
L-rhamnonate
step in the alternative pathway for L-rhamnose metabolism, overview
687737
Scheffersomyces stipitis
2-dehydro-3-deoxy-L-rhamnonate + H2O
-
-
-
?
4.2.1.90
L-rhamnonate
the catalytic mechanism is similar to those of the known acid-sugar dehydratases in this protein family
687737
Azotobacter vinelandii
2-dehydro-3-deoxy-L-rhamnonate + H2O
-
-
-
?
4.2.1.90
L-rhamnonate
-
687737
Debaryomyces hansenii NBRC0083
2-dehydro-3-deoxy-L-rhamnonate + H2O
-
-
-
?
4.2.1.90
L-rhamnonate
step in the alternative pathway for L-rhamnose metabolism, overview
687737
Debaryomyces hansenii NBRC0083
2-dehydro-3-deoxy-L-rhamnonate + H2O
-
-
-
?
4.2.1.90
L-rhamnonate
step in the alternative pathway for L-rhamnose metabolism, overview
687737
Azotobacter vinelandii NBRC 102612
2-dehydro-3-deoxy-L-rhamnonate + H2O
-
-
-
?
4.2.1.90
L-rhamnonate
the catalytic mechanism is similar to those of the known acid-sugar dehydratases in this protein family
687737
Azotobacter vinelandii NBRC 102612
2-dehydro-3-deoxy-L-rhamnonate + H2O
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.1.1.173
?
x * 25000, SDS-PAGE
Scheffersomyces stipitis
4.1.2.53
?
x * about 35000, SDS-PAGE
Azotobacter vinelandii
4.1.2.53
?
x * about 35000, SDS-PAGE
Scheffersomyces stipitis
Synonyms
EC Number
Synonyms
Commentary
Organism
1.1.1.378
AvLRA1
-
Azotobacter vinelandii
3.1.1.65
L-rhamnono-gamma-lactonase
-
Azotobacter vinelandii
3.1.1.65
L-rhamnono-gamma-lactonase
-
Debaryomyces hansenii
3.1.1.65
L-rhamnono-gamma-lactonase
-
Scheffersomyces stipitis
3.1.1.65
LRA2
-
Azotobacter vinelandii
3.1.1.65
LRA2
-
Scheffersomyces stipitis
4.1.2.53
L-KDR aldolase
-
Azotobacter vinelandii
4.1.2.53
L-KDR aldolase
-
Scheffersomyces stipitis
4.1.2.53
LRA4
-
Azotobacter vinelandii
4.1.2.53
LRA4
-
Scheffersomyces stipitis
4.2.1.90
LRA3
-
Azotobacter vinelandii
4.2.1.90
LRA3
-
Debaryomyces hansenii
4.2.1.90
LRA3
-
Scheffersomyces stipitis
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
1.1.1.173
30
-
oxidation assay at
Debaryomyces hansenii
1.1.1.173
30
-
oxidation assay at
Scheffersomyces stipitis
1.1.1.378
30
-
assay at
Azotobacter vinelandii
4.2.1.90
30
-
assay at
Azotobacter vinelandii
4.2.1.90
30
-
assay at
Debaryomyces hansenii
4.2.1.90
30
-
assay at
Scheffersomyces stipitis
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.1.1.173
1510
-
L-rhamnose
pH 9.0, 30C, recombinant enzyme
Scheffersomyces stipitis
1.1.1.173
2860
-
L-rhamnose
pH 9.0, 30C, recombinant enzyme
Debaryomyces hansenii
1.1.1.378
4.8
-
L-fucose
pH 9.0, 30C, cosubstrate: NAD+
Azotobacter vinelandii
1.1.1.378
9.2
-
L-fucose
pH 9.0, 30C, cosubstrate: NADP+
Azotobacter vinelandii
1.1.1.378
9.4
-
L-Mannose
pH 9.0, 30C, cosubstrate: NAD+
Azotobacter vinelandii
1.1.1.378
17
-
L-Mannose
pH 9.0, 30C, cosubstrate: NADP+
Azotobacter vinelandii
1.1.1.378
37.2
-
L-rhamnose
pH 9.0, 30C, cosubstrate: NAD+
Azotobacter vinelandii
1.1.1.378
41.3
-
L-Lyxose
pH 9.0, 30C, cosubstrate: NAD+
Azotobacter vinelandii
1.1.1.378
74.8
-
L-rhamnose
pH 9.0, 30C, cosubstrate: NADP+
Azotobacter vinelandii
1.1.1.378
79.7
-
L-Lyxose
pH 9.0, 30C, cosubstrate: NADP+
Azotobacter vinelandii
4.1.2.53
49.3
-
2-dehydro-3-deoxy-L-rhamnonate
in 50 mM sodium phosphate (pH 7.0), 5 mM Mn2+, temperature not specified in the publication
Scheffersomyces stipitis
4.1.2.53
54.7
-
2-dehydro-3-deoxy-L-rhamnonate
in 50 mM sodium phosphate (pH 7.0), 5 mM Mn2+, temperature not specified in the publication
Azotobacter vinelandii
4.2.1.90
0.72
-
L-Rhamnonate
pH 7.0, 30C
Azotobacter vinelandii
4.2.1.90
49.3
-
L-Rhamnonate
pH 7.0, 30C
Scheffersomyces stipitis
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.173
9
-
oxidation assay at
Debaryomyces hansenii
1.1.1.173
9
-
oxidation assay at
Scheffersomyces stipitis
1.1.1.378
9
-
assay at
Azotobacter vinelandii
4.2.1.90
7
-
assay at
Azotobacter vinelandii
4.2.1.90
7
-
assay at
Debaryomyces hansenii
4.2.1.90
7
-
assay at
Scheffersomyces stipitis
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.173
NAD+
strictly dependent on
Debaryomyces hansenii
1.1.1.173
NAD+
strictly dependent on
Scheffersomyces stipitis
1.1.1.173
NADH
strictly dependent on
Debaryomyces hansenii
1.1.1.173
NADH
strictly dependent on
Scheffersomyces stipitis
1.1.1.378
NAD+
-
Azotobacter vinelandii
1.1.1.378
NADP+
-
Azotobacter vinelandii
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.173
gene LRA1, DNA and amino acid sequence determination and analysis, genetic organization, functional expression of His-tagged enzyme in Escherichia coli
Debaryomyces hansenii
1.1.1.173
gene LRA1, DNA and amino acid sequence determination and analysis, genetic organization, functional expression of His-tagged enzyme in Escherichia coli
Scheffersomyces stipitis
1.1.1.378
expression in Escherichia coli
Azotobacter vinelandii
3.1.1.65
overexpression in Escherichia coli DH5 alpha as His6-tagged enzyme
Azotobacter vinelandii
3.1.1.65
overexpression in Escherichia coli DH5 alpha as His6-tagged enzyme
Debaryomyces hansenii
3.1.1.65
overexpression in Escherichia coli DH5 alpha as His6-tagged enzyme
Scheffersomyces stipitis
4.1.2.53
expressed in Escherichia coli DH5alpha cells
Azotobacter vinelandii
4.1.2.53
expressed in Escherichia coli DH5alpha cells
Scheffersomyces stipitis
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.173
NAD+
strictly dependent on
Debaryomyces hansenii
1.1.1.173
NAD+
strictly dependent on
Scheffersomyces stipitis
1.1.1.173
NADH
strictly dependent on
Debaryomyces hansenii
1.1.1.173
NADH
strictly dependent on
Scheffersomyces stipitis
1.1.1.378
NAD+
-
Azotobacter vinelandii
1.1.1.378
NADP+
-
Azotobacter vinelandii
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.2.53
Ca2+
complete inhibition in the presence of Ca2+
Azotobacter vinelandii
4.1.2.53
Co2+
about 75% residual activity in the presence of Co2+
Azotobacter vinelandii
4.1.2.53
Cu2+
complete inhibition in the presence of Cu2+
Azotobacter vinelandii
4.1.2.53
Fe2+
complete inhibition in the presence of Fe2+
Azotobacter vinelandii
4.1.2.53
Mg2+
about 25% residual activity in the presence of Mg2+
Azotobacter vinelandii
4.1.2.53
Ni2+
about 10% residual activity in the presence of Ni2+
Azotobacter vinelandii
4.1.2.53
Zn2+
about 80% residual activity in the presence of Zn2+
Azotobacter vinelandii
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.173
1.71
-
L-rhamnose
pH 9.0, 30C, recombinant enzyme
Scheffersomyces stipitis
1.1.1.173
9.35
-
L-rhamnose
pH 9.0, 30C, recombinant enzyme
Debaryomyces hansenii
1.1.1.378
2.34
-
L-rhamnose
pH 9.0, 30C, cosubstrate: NADP+
Azotobacter vinelandii
1.1.1.378
2.61
-
L-rhamnose
pH 9.0, 30C, cosubstrate: NAD+
Azotobacter vinelandii
1.1.1.378
4.96
-
L-Lyxose
pH 9.0, 30C, cosubstrate: NADP+
Azotobacter vinelandii
1.1.1.378
8.43
-
L-Lyxose
pH 9.0, 30C, cosubstrate: NAD+
Azotobacter vinelandii
1.1.1.378
89.3
-
L-Mannose
pH 9.0, 30C, cosubstrate: NADP+
Azotobacter vinelandii
1.1.1.378
108
-
L-Mannose
pH 9.0, 30C, cosubstrate: NAD+
Azotobacter vinelandii
1.1.1.378
582
-
L-fucose
pH 9.0, 30C, cosubstrate: NADP+
Azotobacter vinelandii
1.1.1.378
673
-
L-fucose
pH 9.0, 30C, cosubstrate: NAD+
Azotobacter vinelandii
4.1.2.53
0.915
-
2-dehydro-3-deoxy-L-rhamnonate
in 50 mM sodium phosphate (pH 7.0), 5 mM Mn2+, temperature not specified in the publication
Azotobacter vinelandii
4.1.2.53
1.37
-
2-dehydro-3-deoxy-L-rhamnonate
in 50 mM sodium phosphate (pH 7.0), 5 mM Mn2+, temperature not specified in the publication
Scheffersomyces stipitis
4.2.1.90
0.115
-
L-Rhamnonate
pH 7.0, 30C
Azotobacter vinelandii
4.2.1.90
1.37
-
L-Rhamnonate
pH 7.0, 30C
Scheffersomyces stipitis
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.1.2.53
Co2+
about 240% activity in the presence of Co2+
Scheffersomyces stipitis
4.1.2.53
Mg2+
about 150% activity in the presence of Co2+
Scheffersomyces stipitis
4.1.2.53
Mg2+
more than 250% activity in the presence of Mg2+, 5 mM MgCl2 used in assay conditions
Scheffersomyces stipitis
4.1.2.53
additional information
not stimulated by Mg2+, Co2+, Ni2+, Zn2+, Fe2+, Cu2+, and Ca2+
Azotobacter vinelandii
4.1.2.53
additional information
not stimulated by Zn2+, Fe2+, Cu2+, and Ca2+
Scheffersomyces stipitis
4.1.2.53
Ni2+
about 140% activity in the presence of Ni2+
Scheffersomyces stipitis
4.2.1.90
Mg2+
-
Azotobacter vinelandii
4.2.1.90
Mg2+
-
Debaryomyces hansenii
4.2.1.90
Mg2+
-
Scheffersomyces stipitis
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.173
25000
-
x * 25000, SDS-PAGE
Scheffersomyces stipitis
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.1.1.173
L-rhamnose + NAD+
Debaryomyces hansenii
-
L-rhamno-1,4-lactone + NADH
-
-
r
1.1.1.173
L-rhamnose + NAD+
Scheffersomyces stipitis
-
L-rhamno-1,4-lactone + NADH
-
-
r
1.1.1.173
L-rhamnose + NAD+
Debaryomyces hansenii NBRC0083
-
L-rhamno-1,4-lactone + NADH
-
-
r
1.1.1.378
L-rhamnose + NAD(P)+
Azotobacter vinelandii
first enzyme of the non-phosphorylative L-rhamnose pathway in archaea, that is analogous to the Entner-Doudoroff pathway
L-rhamno-1,4-lactone + NAD(P)H + H+
-
-
?
3.1.1.65
L-rhamnono-1,4-lactone + H2O
Azotobacter vinelandii
-
L-rhamnonate
-
-
?
3.1.1.65
L-rhamnono-1,4-lactone + H2O
Debaryomyces hansenii
-
L-rhamnonate
-
-
?
3.1.1.65
L-rhamnono-1,4-lactone + H2O
Scheffersomyces stipitis
-
L-rhamnonate
-
-
?
3.1.1.65
L-rhamnono-1,4-lactone + H2O
Debaryomyces hansenii NBRC0083
-
L-rhamnonate
-
-
?
3.1.1.65
L-rhamnono-1,4-lactone + H2O
Azotobacter vinelandii NBRC 102612
-
L-rhamnonate
-
-
?
4.1.2.53
2-dehydro-3-deoxy-L-rhamnonate
Azotobacter vinelandii
preferred substrate
pyruvate + (R)-lactaldehyde
-
-
?
4.1.2.53
2-dehydro-3-deoxy-L-rhamnonate
Scheffersomyces stipitis
preferred substrate
pyruvate + (R)-lactaldehyde
-
-
?
4.2.1.90
L-rhamnonate
Azotobacter vinelandii
step in the alternative pathway for L-rhamnose metabolism, overview
2-dehydro-3-deoxy-L-rhamnonate + H2O
-
-
?
4.2.1.90
L-rhamnonate
Debaryomyces hansenii
step in the alternative pathway for L-rhamnose metabolism, overview
2-dehydro-3-deoxy-L-rhamnonate + H2O
-
-
?
4.2.1.90
L-rhamnonate
Scheffersomyces stipitis
step in the alternative pathway for L-rhamnose metabolism, overview
2-dehydro-3-deoxy-L-rhamnonate + H2O
-
-
?
4.2.1.90
L-rhamnonate
Debaryomyces hansenii NBRC0083
step in the alternative pathway for L-rhamnose metabolism, overview
2-dehydro-3-deoxy-L-rhamnonate + H2O
-
-
?
4.2.1.90
L-rhamnonate
Azotobacter vinelandii NBRC 102612
step in the alternative pathway for L-rhamnose metabolism, overview
2-dehydro-3-deoxy-L-rhamnonate + H2O
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.173
native enzyme 42.8fold by anion exchange chromatography, ultrafiltration, gel filtration, hydroxyapatite chromatography, and again gel filtration, recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography
Scheffersomyces stipitis
1.1.1.173
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography
Debaryomyces hansenii
1.1.1.378
-
Azotobacter vinelandii
3.1.1.65
nickel-nitrilotriacetic acid superflow column
Azotobacter vinelandii
3.1.1.65
nickel-nitrilotriacetic acid superflow column
Debaryomyces hansenii
3.1.1.65
nickel-nitrilotriacetic acid superflow column
Scheffersomyces stipitis
4.1.2.53
Ni-NTA column chromatography
Azotobacter vinelandii
4.1.2.53
Ni-NTA column chromatography
Scheffersomyces stipitis
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
1.1.1.173
29.9
-
purified native enzyme
Scheffersomyces stipitis
1.1.1.173
50.4
-
purified recombinant enzyme
Scheffersomyces stipitis
1.1.1.173
53.3
-
purified recombinant enzyme
Debaryomyces hansenii
1.1.1.378
0.15
-
pH 9.0, 30C, substrates: L-fucose, NAD+
Azotobacter vinelandii
1.1.1.378
0.27
-
pH 9.0, 30C, substrates: L-fucose, NADP+
Azotobacter vinelandii
1.1.1.378
1.69
-
pH 9.0, 30C, substrates: L-mannose, NAD+
Azotobacter vinelandii
1.1.1.378
3.64
-
pH 9.0, 30C, substrates: L-mannose, NADP+
Azotobacter vinelandii
1.1.1.378
50.7
-
pH 9.0, 30C, substrates: L-lyxose, NAD+
Azotobacter vinelandii
1.1.1.378
72.1
-
pH 9.0, 30C, substrates: L-rhamnose, NAD+
Azotobacter vinelandii
1.1.1.378
125
-
pH 9.0, 30C, substrates: L-lyxose, NADP+
Azotobacter vinelandii
1.1.1.378
163
-
pH 9.0, 30C, substrates: L-rhamnose, NADP+
Azotobacter vinelandii
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1.1.1.173
L-rhamnose + NAD+
-
687737
Debaryomyces hansenii
L-rhamno-1,4-lactone + NADH
-
-
-
r
1.1.1.173
L-rhamnose + NAD+
-
687737
Scheffersomyces stipitis
L-rhamno-1,4-lactone + NADH
-
-
-
r
1.1.1.173
L-rhamnose + NAD+
-
687737
Debaryomyces hansenii NBRC0083
L-rhamno-1,4-lactone + NADH
-
-
-
r
1.1.1.378
L-fucose + NAD+
the enzyme shows significant preference for NADP+ over NAD+
687737
Azotobacter vinelandii
?
-
-
-
?
1.1.1.378
L-fucose + NADP+
the enzyme shows significant preference for NADP+ over NAD+
687737
Azotobacter vinelandii
?
-
-
-
?
1.1.1.378
L-lyxose + NAD+
the enzyme shows significant preference for NADP+ over NAD+
687737
Azotobacter vinelandii
?
-
-
-
?
1.1.1.378
L-lyxose + NADP+
the enzyme shows significant preference for NADP+ over NAD+
687737
Azotobacter vinelandii
?
-
-
-
?
1.1.1.378
L-mannose + NAD+
the enzyme shows significant preference for NADP+ over NAD+
687737
Azotobacter vinelandii
?
-
-
-
?
1.1.1.378
L-mannose + NADP+
the enzyme shows significant preference for NADP+ over NAD+
687737
Azotobacter vinelandii
?
-
-
-
?
1.1.1.378
L-rhamnose + NAD(P)+
first enzyme of the non-phosphorylative L-rhamnose pathway in archaea, that is analogous to the Entner-Doudoroff pathway
687737
Azotobacter vinelandii
L-rhamno-1,4-lactone + NAD(P)H + H+
-
-
-
?
1.1.1.378
L-rhamnose + NAD+
the enzyme shows significant preference for NADP+ over NAD+
687737
Azotobacter vinelandii
L-rhamno-1,4-lactone + NADH + H+
-
-
-
?
1.1.1.378
L-rhamnose + NADP+
the enzyme shows significant preference for NADP+ over NAD+
687737
Azotobacter vinelandii
L-rhamno-1,4-lactone + NADPH + H+
-
-
-
?
3.1.1.65
L-rhamnono-1,4-lactone + H2O
-
687737
Azotobacter vinelandii
L-rhamnonate
-
-
-
?
3.1.1.65
L-rhamnono-1,4-lactone + H2O
-
687737
Debaryomyces hansenii
L-rhamnonate
-
-
-
?
3.1.1.65
L-rhamnono-1,4-lactone + H2O
-
687737
Scheffersomyces stipitis
L-rhamnonate
-
-
-
?
3.1.1.65
L-rhamnono-1,4-lactone + H2O
-
687737
Debaryomyces hansenii NBRC0083
L-rhamnonate
-
-
-
?
3.1.1.65
L-rhamnono-1,4-lactone + H2O
-
687737
Azotobacter vinelandii NBRC 102612
L-rhamnonate
-
-
-
?
4.1.2.53
2-dehydro-3-deoxy-L-mannonate
13.3% activity compared to 2-dehydro-3-deoxy-L-rhamnonate
687737
Scheffersomyces stipitis
?
-
-
-
?
4.1.2.53
2-dehydro-3-deoxy-L-mannonate
6.7% activity compared to 2-dehydro-3-deoxy-L-rhamnonate
687737
Azotobacter vinelandii
?
-
-
-
?
4.1.2.53
2-dehydro-3-deoxy-L-rhamnonate
preferred substrate
687737
Azotobacter vinelandii
pyruvate + (R)-lactaldehyde
-
-
-
?
4.1.2.53
2-dehydro-3-deoxy-L-rhamnonate
preferred substrate
687737
Scheffersomyces stipitis
pyruvate + (R)-lactaldehyde
-
-
-
?
4.1.2.53
2-dehydro-3-deoxy-L-xylonate
54.7% activity compared to 2-dehydro-3-deoxy-L-rhamnonate
687737
Scheffersomyces stipitis
?
-
-
-
?
4.1.2.53
2-dehydro-3-deoxy-L-xylonate
7.6% activity compared to 2-dehydro-3-deoxy-L-rhamnonate
687737
Azotobacter vinelandii
?
-
-
-
?
4.2.1.90
L-lyxonate
-
687737
Azotobacter vinelandii
2-dehydro-3-deoxy-L-lyxonate + H2O
-
-
-
?
4.2.1.90
L-lyxonate
-
687737
Azotobacter vinelandii NBRC 102612
2-dehydro-3-deoxy-L-lyxonate + H2O
-
-
-
?
4.2.1.90
L-mannonate
-
687737
Azotobacter vinelandii
2-dehydro-3-deoxy-L-mannonate + H2O
-
-
-
?
4.2.1.90
L-mannonate
-
687737
Azotobacter vinelandii NBRC 102612
2-dehydro-3-deoxy-L-mannonate + H2O
-
-
-
?
4.2.1.90
L-rhamnonate
-
687737
Debaryomyces hansenii
2-dehydro-3-deoxy-L-rhamnonate + H2O
-
-
-
?
4.2.1.90
L-rhamnonate
-
687737
Scheffersomyces stipitis
2-dehydro-3-deoxy-L-rhamnonate + H2O
-
-
-
?
4.2.1.90
L-rhamnonate
step in the alternative pathway for L-rhamnose metabolism, overview
687737
Azotobacter vinelandii
2-dehydro-3-deoxy-L-rhamnonate + H2O
-
-
-
?
4.2.1.90
L-rhamnonate
step in the alternative pathway for L-rhamnose metabolism, overview
687737
Debaryomyces hansenii
2-dehydro-3-deoxy-L-rhamnonate + H2O
-
-
-
?
4.2.1.90
L-rhamnonate
step in the alternative pathway for L-rhamnose metabolism, overview
687737
Scheffersomyces stipitis
2-dehydro-3-deoxy-L-rhamnonate + H2O
-
-
-
?
4.2.1.90
L-rhamnonate
the catalytic mechanism is similar to those of the known acid-sugar dehydratases in this protein family
687737
Azotobacter vinelandii
2-dehydro-3-deoxy-L-rhamnonate + H2O
-
-
-
?
4.2.1.90
L-rhamnonate
-
687737
Debaryomyces hansenii NBRC0083
2-dehydro-3-deoxy-L-rhamnonate + H2O
-
-
-
?
4.2.1.90
L-rhamnonate
step in the alternative pathway for L-rhamnose metabolism, overview
687737
Debaryomyces hansenii NBRC0083
2-dehydro-3-deoxy-L-rhamnonate + H2O
-
-
-
?
4.2.1.90
L-rhamnonate
step in the alternative pathway for L-rhamnose metabolism, overview
687737
Azotobacter vinelandii NBRC 102612
2-dehydro-3-deoxy-L-rhamnonate + H2O
-
-
-
?
4.2.1.90
L-rhamnonate
the catalytic mechanism is similar to those of the known acid-sugar dehydratases in this protein family
687737
Azotobacter vinelandii NBRC 102612
2-dehydro-3-deoxy-L-rhamnonate + H2O
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.1.1.173
?
x * 25000, SDS-PAGE
Scheffersomyces stipitis
4.1.2.53
?
x * about 35000, SDS-PAGE
Azotobacter vinelandii
4.1.2.53
?
x * about 35000, SDS-PAGE
Scheffersomyces stipitis
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
1.1.1.173
30
-
oxidation assay at
Debaryomyces hansenii
1.1.1.173
30
-
oxidation assay at
Scheffersomyces stipitis
1.1.1.378
30
-
assay at
Azotobacter vinelandii
4.2.1.90
30
-
assay at
Azotobacter vinelandii
4.2.1.90
30
-
assay at
Debaryomyces hansenii
4.2.1.90
30
-
assay at
Scheffersomyces stipitis
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.1.1.173
1510
-
L-rhamnose
pH 9.0, 30C, recombinant enzyme
Scheffersomyces stipitis
1.1.1.173
2860
-
L-rhamnose
pH 9.0, 30C, recombinant enzyme
Debaryomyces hansenii
1.1.1.378
4.8
-
L-fucose
pH 9.0, 30C, cosubstrate: NAD+
Azotobacter vinelandii
1.1.1.378
9.2
-
L-fucose
pH 9.0, 30C, cosubstrate: NADP+
Azotobacter vinelandii
1.1.1.378
9.4
-
L-Mannose
pH 9.0, 30C, cosubstrate: NAD+
Azotobacter vinelandii
1.1.1.378
17
-
L-Mannose
pH 9.0, 30C, cosubstrate: NADP+
Azotobacter vinelandii
1.1.1.378
37.2
-
L-rhamnose
pH 9.0, 30C, cosubstrate: NAD+
Azotobacter vinelandii
1.1.1.378
41.3
-
L-Lyxose
pH 9.0, 30C, cosubstrate: NAD+
Azotobacter vinelandii
1.1.1.378
74.8
-
L-rhamnose
pH 9.0, 30C, cosubstrate: NADP+
Azotobacter vinelandii
1.1.1.378
79.7
-
L-Lyxose
pH 9.0, 30C, cosubstrate: NADP+
Azotobacter vinelandii
4.1.2.53
49.3
-
2-dehydro-3-deoxy-L-rhamnonate
in 50 mM sodium phosphate (pH 7.0), 5 mM Mn2+, temperature not specified in the publication
Scheffersomyces stipitis
4.1.2.53
54.7
-
2-dehydro-3-deoxy-L-rhamnonate
in 50 mM sodium phosphate (pH 7.0), 5 mM Mn2+, temperature not specified in the publication
Azotobacter vinelandii
4.2.1.90
0.72
-
L-Rhamnonate
pH 7.0, 30C
Azotobacter vinelandii
4.2.1.90
49.3
-
L-Rhamnonate
pH 7.0, 30C
Scheffersomyces stipitis
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.173
9
-
oxidation assay at
Debaryomyces hansenii
1.1.1.173
9
-
oxidation assay at
Scheffersomyces stipitis
1.1.1.378
9
-
assay at
Azotobacter vinelandii
4.2.1.90
7
-
assay at
Azotobacter vinelandii
4.2.1.90
7
-
assay at
Debaryomyces hansenii
4.2.1.90
7
-
assay at
Scheffersomyces stipitis
General Information
EC Number
General Information
Commentary
Organism
1.1.1.378
physiological function
first enzyme of the non-phosphorylative L-rhamnose pathway in archaea, that is analogous to the Entner-Doudoroff pathway
Azotobacter vinelandii
General Information (protein specific)
EC Number
General Information
Commentary
Organism
1.1.1.378
physiological function
first enzyme of the non-phosphorylative L-rhamnose pathway in archaea, that is analogous to the Entner-Doudoroff pathway
Azotobacter vinelandii
KCat/KM [mM/s]
EC Number
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
1.1.1.378
0.007
-
L-fucose
pH 9.0, 30C, cosubstrate: NAD+
Azotobacter vinelandii
1.1.1.378
0.016
-
L-fucose
pH 9.0, 30C, cosubstrate: NADP+
Azotobacter vinelandii
1.1.1.378
0.09
-
L-Mannose
pH 9.0, 30C, cosubstrate: NAD+
Azotobacter vinelandii
1.1.1.378
0.19
-
L-Mannose
pH 9.0, 30C, cosubstrate: NADP+
Azotobacter vinelandii
1.1.1.378
4.9
-
L-Lyxose
pH 9.0, 30C, cosubstrate: NAD+
Azotobacter vinelandii
1.1.1.378
14.3
-
L-rhamnose
pH 9.0, 30C, cosubstrate: NAD+
Azotobacter vinelandii
1.1.1.378
16.05
-
L-Lyxose
pH 9.0, 30C, cosubstrate: NADP+
Azotobacter vinelandii
1.1.1.378
35.7
-
L-rhamnose
pH 9.0, 30C, cosubstrate: NADP+
Azotobacter vinelandii
4.1.2.53
36.2
-
2-dehydro-3-deoxy-L-rhamnonate
in 50 mM sodium phosphate (pH 7.0), 5 mM Mn2+, temperature not specified in the publication
Scheffersomyces stipitis
4.1.2.53
60.2
-
2-dehydro-3-deoxy-L-rhamnonate
in 50 mM sodium phosphate (pH 7.0), 5 mM Mn2+, temperature not specified in the publication
Azotobacter vinelandii
KCat/KM [mM/s] (protein specific)
EC Number
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
1.1.1.378
0.007
-
L-fucose
pH 9.0, 30C, cosubstrate: NAD+
Azotobacter vinelandii
1.1.1.378
0.016
-
L-fucose
pH 9.0, 30C, cosubstrate: NADP+
Azotobacter vinelandii
1.1.1.378
0.09
-
L-Mannose
pH 9.0, 30C, cosubstrate: NAD+
Azotobacter vinelandii
1.1.1.378
0.19
-
L-Mannose
pH 9.0, 30C, cosubstrate: NADP+
Azotobacter vinelandii
1.1.1.378
4.9
-
L-Lyxose
pH 9.0, 30C, cosubstrate: NAD+
Azotobacter vinelandii
1.1.1.378
14.3
-
L-rhamnose
pH 9.0, 30C, cosubstrate: NAD+
Azotobacter vinelandii
1.1.1.378
16.05
-
L-Lyxose
pH 9.0, 30C, cosubstrate: NADP+
Azotobacter vinelandii
1.1.1.378
35.7
-
L-rhamnose
pH 9.0, 30C, cosubstrate: NADP+
Azotobacter vinelandii
4.1.2.53
36.2
-
2-dehydro-3-deoxy-L-rhamnonate
in 50 mM sodium phosphate (pH 7.0), 5 mM Mn2+, temperature not specified in the publication
Scheffersomyces stipitis
4.1.2.53
60.2
-
2-dehydro-3-deoxy-L-rhamnonate
in 50 mM sodium phosphate (pH 7.0), 5 mM Mn2+, temperature not specified in the publication
Azotobacter vinelandii