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Literature summary extracted from

  • Das, A.; Davis, M.A.; Tomoda, H.; Omura, S.; Rudel, L.L.
    Identification of the interaction site within acyl-CoA:cholesterol acyltransferase 2 for the isoform-specific inhibitor pyripyropene A (2008), J. Biol. Chem., 283, 10453-10460.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.26 all constructs, full-length, chimeras and mutants are cloned into the pCDNA3 vector, AC29 cells are transfected Chlorocebus aethiops

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.26 A1:1-123 chimeric protein A1(1-123)-A2(105-526) Chlorocebus aethiops
2.3.1.26 A1:1-385 chimeric protein A1(1-385)-A2(364-440)-A1(463-550) Chlorocebus aethiops
2.3.1.26 A1:1-71 chimeric protein A1(1-71)-A2(70-562) Chlorocebus aethiops
2.3.1.26 A2:1-227 chimeric protein A2(1-227)-A1(248-489)-A2(468-526) Chlorocebus aethiops
2.3.1.26 A2:1-249 chimeric protein A2(1-249)-A1(270-362)-A2(341-526) Chlorocebus aethiops
2.3.1.26 A2:1-363 chimeric protein A2(1-363)-A1(386-462)-A2(441-526) Chlorocebus aethiops
2.3.1.26 A2:1-428 chimeric protein A2(1-428)-A1(444-550) Chlorocebus aethiops
2.3.1.26 A2:1-440 chimeric protein A2(1-440)-A1(463-502)-A2(480-526) Chlorocebus aethiops
2.3.1.26 A2:1-479 chimeric protein A2(1-479)-A1(502-550) Chlorocebus aethiops
2.3.1.26 A2C497S A2 mutant Chlorocebus aethiops
2.3.1.26 A2I491V A2 mutant Chlorocebus aethiops
2.3.1.26 A2L480M A2 mutant Chlorocebus aethiops
2.3.1.26 A2L495F A2 mutant Chlorocebus aethiops
2.3.1.26 A2M484S A2 mutant Chlorocebus aethiops
2.3.1.26 A2Q489N A2 mutant Chlorocebus aethiops
2.3.1.26 A2Q492L A2 mutant Chlorocebus aethiops
2.3.1.26 A2R504Q A2 mutant Chlorocebus aethiops
2.3.1.26 A2S494C A2 mutant Chlorocebus aethiops
2.3.1.26 A2V493L A2 mutant Chlorocebus aethiops
2.3.1.26 Ch1 chimeric protein A2(1-428)-A1(451-462)-A2(441-526) Chlorocebus aethiops
2.3.1.26 CT504 A2(1-504) Chlorocebus aethiops

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.26 pyripyropene A
-
Chlorocebus aethiops

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.26 0.00000266
-
cis-9-octadecenoyl-CoA pyripyropene A-insensitive chimera A2:1-479 Chlorocebus aethiops
2.3.1.26 0.00000333
-
cis-9-octadecenoyl-CoA pyripyropene A-insensitive chimera A2:1-428 Chlorocebus aethiops
2.3.1.26 0.00000553
-
cis-9-octadecenoyl-CoA wild typeACAT2 Chlorocebus aethiops
2.3.1.26 0.00000587
-
cis-9-octadecenoyl-CoA pyripyropene A-insensitive mutant A2S494C Chlorocebus aethiops
2.3.1.26 0.000008094
-
cis-9-octadecenoyl-CoA pyripyropene A-insensitive mutant A2V493L Chlorocebus aethiops
2.3.1.26 0.00001218
-
cis-9-octadecenoyl-CoA pyripyropene A-insensitive mutant A2Q492L Chlorocebus aethiops

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.26 acyl-CoA + cholesterol Chlorocebus aethiops
-
CoA + cholesterol ester
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.26 Chlorocebus aethiops
-
African green monkey
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.26 microsomes from AC29 cells are prepared Chlorocebus aethiops

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.26 acyl-CoA + cholesterol
-
Chlorocebus aethiops CoA + cholesterol ester
-
?
2.3.1.26 oleoyl-CoA + cholesterol
-
Chlorocebus aethiops CoA + cholesteryl oleate
-
?
2.3.1.26 oleoyl-CoA + cholesterol activity assay Chlorocebus aethiops ?
-
?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.26 A2
-
Chlorocebus aethiops
2.3.1.26 ACAT2
-
Chlorocebus aethiops
2.3.1.26 acyl-CoA:cholesterol acyltransferase 2
-
Chlorocebus aethiops

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.26 37
-
microsomal ACAT assay Chlorocebus aethiops