BRENDA - Enzyme Database

Ubiquinone-binding site mutations in the Saccharomyces cerevisiae succinate dehydrogenase generate superoxide and lead to the accumulation of succinate

Szeto, S.S.; Reinke, S.N.; Sykes, B.D.; Lemire, B.D.; J. Biol. Chem. 282, 27518-27526 (2007)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
1.3.5.1
phenazine methosulfate
-
Saccharomyces cerevisiae
Cloned(Commentary)
EC Number
Commentary
Organism
1.3.5.1
genes SDHA, SDHB, SDHC, and SDHD
Saccharomyces cerevisiae
Engineering
EC Number
Amino acid exchange
Commentary
Organism
1.3.5.1
D88E
site-directed mutagenesis of a subunit Sdh4p residue, the mutant shows reduced activity, as well as accumulation and secretion of succinate, the mutant is sensitive to hyperoxia and paraquate and shows enhanced superoxide production in vivo and in vitro
Saccharomyces cerevisiae
1.3.5.1
D88K
site-directed mutagenesis of a subunit Sdh4p residue, the mutant shows reduced activity and FAD content
Saccharomyces cerevisiae
1.3.5.1
D88N
site-directed mutagenesis of a subunit Sdh4p residue, the mutant shows reduced activity and FAD content, as well as accumulation and secretion of succinate, the mutant is sensitive to hyperoxia and paraquate and shows enhanced superoxide production in vivo and in vitro
Saccharomyces cerevisiae
1.3.5.1
additional information
ubiquinone-binding site mutations in the succinate dehydrogenase generate superoxide and lead to the accumulation of succinate
Saccharomyces cerevisiae
1.3.5.1
R47C
site-directed mutagenesis of a subunit Sdh3p residue, the mutant shows reduced activity
Saccharomyces cerevisiae
1.3.5.1
R47E
site-directed mutagenesis of a subunit Sdh3p residue, the mutant shows reduced activity and FAD content
Saccharomyces cerevisiae
1.3.5.1
R47K
site-directed mutagenesis of a subunit Sdh3p residue, the mutant shows reduced activity, as well as accumulation and secretion of succinate, the mutant is sensitive to hyperoxia and paraquate and shows enhanced superoxide production in vivo and in vitro
Saccharomyces cerevisiae
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.3.5.1
malonate
-
Saccharomyces cerevisiae
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.3.5.1
mitochondrial membrane
-
Saccharomyces cerevisiae
31966
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.3.5.1
succinate + ubiquinone
Saccharomyces cerevisiae
SDH is an essential component of the electron transport chain and of the tricarboxylic acid cycle in the mitochondrial membrane
fumarate + ubiquinol
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.3.5.1
Saccharomyces cerevisiae
-
genes SDHA, SDHB, SDHC, and SDHD encding the four subunits of the enzyme
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
1.3.5.1
additional information
-
substrate specificities of wild-type and mutant enzymes, overview
Saccharomyces cerevisiae
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.3.5.1
succinate + decylubiquinone
decylubiquinone-mediated reduction of dichlorophenol indophenol
687608
Saccharomyces cerevisiae
fumarate + decylubiquinol
-
-
-
?
1.3.5.1
succinate + oxidized 2,6-dichlorophenolindophenol
succinate-dependent, phenazine methosulfate-mediated malonate-sensitive reduction of 2,6-dichlorophenol indophenol
687608
Saccharomyces cerevisiae
fumarate + reduced 2,6-dichlorophenolindophenol
-
-
-
?
1.3.5.1
succinate + ubiquinone
SDH is an essential component of the electron transport chain and of the tricarboxylic acid cycle in the mitochondrial membrane
687608
Saccharomyces cerevisiae
fumarate + ubiquinol
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.3.5.1
tetramer
subunits SdhA, SdhB, SdhC, and SdhD
Saccharomyces cerevisiae
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.3.5.1
2,6-dichlorophenolindophenol
-
Saccharomyces cerevisiae
1.3.5.1
decylubiquinone
-
Saccharomyces cerevisiae
1.3.5.1
FAD
-
Saccharomyces cerevisiae
1.3.5.1
ubiquinone
-
Saccharomyces cerevisiae
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
1.3.5.1
phenazine methosulfate
-
Saccharomyces cerevisiae
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.3.5.1
genes SDHA, SDHB, SDHC, and SDHD
Saccharomyces cerevisiae
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.3.5.1
2,6-dichlorophenolindophenol
-
Saccharomyces cerevisiae
1.3.5.1
decylubiquinone
-
Saccharomyces cerevisiae
1.3.5.1
FAD
-
Saccharomyces cerevisiae
1.3.5.1
ubiquinone
-
Saccharomyces cerevisiae
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
1.3.5.1
D88E
site-directed mutagenesis of a subunit Sdh4p residue, the mutant shows reduced activity, as well as accumulation and secretion of succinate, the mutant is sensitive to hyperoxia and paraquate and shows enhanced superoxide production in vivo and in vitro
Saccharomyces cerevisiae
1.3.5.1
D88K
site-directed mutagenesis of a subunit Sdh4p residue, the mutant shows reduced activity and FAD content
Saccharomyces cerevisiae
1.3.5.1
D88N
site-directed mutagenesis of a subunit Sdh4p residue, the mutant shows reduced activity and FAD content, as well as accumulation and secretion of succinate, the mutant is sensitive to hyperoxia and paraquate and shows enhanced superoxide production in vivo and in vitro
Saccharomyces cerevisiae
1.3.5.1
additional information
ubiquinone-binding site mutations in the succinate dehydrogenase generate superoxide and lead to the accumulation of succinate
Saccharomyces cerevisiae
1.3.5.1
R47C
site-directed mutagenesis of a subunit Sdh3p residue, the mutant shows reduced activity
Saccharomyces cerevisiae
1.3.5.1
R47E
site-directed mutagenesis of a subunit Sdh3p residue, the mutant shows reduced activity and FAD content
Saccharomyces cerevisiae
1.3.5.1
R47K
site-directed mutagenesis of a subunit Sdh3p residue, the mutant shows reduced activity, as well as accumulation and secretion of succinate, the mutant is sensitive to hyperoxia and paraquate and shows enhanced superoxide production in vivo and in vitro
Saccharomyces cerevisiae
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.3.5.1
malonate
-
Saccharomyces cerevisiae
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.3.5.1
mitochondrial membrane
-
Saccharomyces cerevisiae
31966
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.3.5.1
succinate + ubiquinone
Saccharomyces cerevisiae
SDH is an essential component of the electron transport chain and of the tricarboxylic acid cycle in the mitochondrial membrane
fumarate + ubiquinol
-
-
?
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
1.3.5.1
additional information
-
substrate specificities of wild-type and mutant enzymes, overview
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.3.5.1
succinate + decylubiquinone
decylubiquinone-mediated reduction of dichlorophenol indophenol
687608
Saccharomyces cerevisiae
fumarate + decylubiquinol
-
-
-
?
1.3.5.1
succinate + oxidized 2,6-dichlorophenolindophenol
succinate-dependent, phenazine methosulfate-mediated malonate-sensitive reduction of 2,6-dichlorophenol indophenol
687608
Saccharomyces cerevisiae
fumarate + reduced 2,6-dichlorophenolindophenol
-
-
-
?
1.3.5.1
succinate + ubiquinone
SDH is an essential component of the electron transport chain and of the tricarboxylic acid cycle in the mitochondrial membrane
687608
Saccharomyces cerevisiae
fumarate + ubiquinol
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.3.5.1
tetramer
subunits SdhA, SdhB, SdhC, and SdhD
Saccharomyces cerevisiae