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Literature summary extracted from

  • Ha, S.W.; Korbas, M.; Klepsch, M.; Meyer-Klaucke, W.; Meyer, O.; Svetlitchnyi, V.
    Interaction of potassium cyanide with the [Ni-4Fe-5S] active site cluster of CO dehydrogenase from Carboxydothermus hydrogenoformans (2007), J. Biol. Chem., 282, 10639-10646.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.7.4 cyanide 0.075 mM, reversible, competitive inhibition of reduced CODHII with respect to the substrate carbon monoxide, which protects reduced CODHII against inhibition by cyanide, inhibited CODHII regains initial activity after a 15-25-min incubation at 70°C with dithionite or Ti(III) citrate under CO or N2, while slower and partial reactivation to 30-50% of the initial activity occurs with dithiothreitol or without reductants Carboxydothermus hydrogenoformans
1.2.7.4 potassium cyanide competitive inhibitor of reduced CODHII with respect to the substrate CO, inhibition of dithionite- or Ti(III) citrate-reduced CODHII by potassium cyanide is fully reversible since the enzyme can be completely reactivated, sodium sulfide has no effect on the reactivation of cyanide-inhibited CODHII in the presence of dithionite Carboxydothermus hydrogenoformans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.7.4 Fe2+ contains one Ni-4Fe-5S cluster Carboxydothermus hydrogenoformans
1.2.7.4 Ni2+ contains one Ni-4Fe-5S cluster Carboxydothermus hydrogenoformans

Organism

EC Number Organism UniProt Comment Textmining
1.2.7.4 Carboxydothermus hydrogenoformans
-
 strain Z-2901 (DSM 6008)
-
1.2.7.4 Carboxydothermus hydrogenoformans Z-2901 / DSM 6008
-
 strain Z-2901 (DSM 6008)
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.7.4 CO + H2O + methyl viologen
-
 Carboxydothermus hydrogenoformans CO2 + reduced methyl viologen
-
 ?
1.2.7.4 CO + H2O + methyl viologen
-
 Carboxydothermus hydrogenoformans Z-2901 / DSM 6008 CO2 + reduced methyl viologen
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.2.7.4 CO dehydrogenase
-
 Carboxydothermus hydrogenoformans
1.2.7.4 CODHII
-
 Carboxydothermus hydrogenoformans
1.2.7.4 Ni-Fe carbon monoxide dehydrogenase II
-
 Carboxydothermus hydrogenoformans

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.7.4 Ni-4Fe-5S center active site cluster Carboxydothermus hydrogenoformans

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.2.7.4 0.0217
-
 cyanide at 23°C, in 4 mM dithionite, and 4 mM dithiothreitol Carboxydothermus hydrogenoformans