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Literature summary extracted from

  • Shi, D.; Morizono, H.; Cabrera-Luque, J.; Yu, X.; Roth, L.; Malamy, M.H.; Allewell, N.M.; Tuchman, M.
    Structure and catalytic mechanism of a novel N-succinyl-L-ornithine transcarbamylase in arginine biosynthesis of Bacteroides fragilis (2006), J. Biol. Chem., 281, 20623-20631.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.1.3.11 in complex with carbamyl phosphate and N-succinyl-L-norvaline, as well as sulfate and N-succinyl-L-norvaline, diffraction to 2.9 and 2.8 A resolution, respectively Bacteroides fragilis

Protein Variants

EC Number Protein Variants Comment Organism
2.1.3.11 P90E/T242L contrary to wild-type, mutant accepts N-acetyl-L-ornithine as a substrate. Decrease in Km value for N-succinyl-L-ornithine Bacteroides fragilis
2.1.3.11 T242L slight decrease in Km value Bacteroides fragilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.3.11 0.0016
-
 N-succinyl-L-ornithine mutant P90E/T242L, pH 8.3, 25°C Bacteroides fragilis
2.1.3.11 0.0119
-
 N-acetyl-L-ornithine mutant P90E/T242L, pH 8.3, 25°C Bacteroides fragilis
2.1.3.11 0.195
-
 N-succinyl-L-ornithine mutant T242L, pH 8.3, 25°C Bacteroides fragilis
2.1.3.11 0.238
-
 N-succinyl-L-ornithine wild-type, pH 8.3, 25°C Bacteroides fragilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.3.11 additional information Bacteroides fragilis Bacteroides fragilis uses N-succinyl-L-ornithine rather than N-acetyl-L-ornithine for de novo arginine biosynthesis ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.3.11 Bacteroides fragilis Q8A1E9
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.3.11 additional information Bacteroides fragilis uses N-succinyl-L-ornithine rather than N-acetyl-L-ornithine for de novo arginine biosynthesis Bacteroides fragilis ?
-
 ?
2.1.3.11 additional information no substrate: L-ornithine Bacteroides fragilis ?
-
 ?
2.1.3.11 N-acetyl-L-ornithine + carbamoyl phosphate no substrate for wild-type, but for mutant P90E/T242L Bacteroides fragilis N-acetyl-L-citrulline + phosphate
-
 ?
2.1.3.11 N-succinyl-L-ornithine + carbamoyl phosphate
-
 Bacteroides fragilis N-succinyl-L-citrulline + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.3.11 ArgF
-
 Bacteroides fragilis