EC Number | General Stability | Organism |
---|---|---|
4.1.1.88 | enzyme is sensitive to repeated freezing and thawing | Acinetobacter calcoaceticus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
4.1.1.88 | 2-Nitro-5-thiocyanobenzoic acid | 0.64 mM, 2.1% residual activity | Acinetobacter calcoaceticus | |
4.1.1.88 | 5,5'-dithio-bis(2-nitrobenzoic acid) | 0.5 mM, 14.5% residual activity | Acinetobacter calcoaceticus | |
4.1.1.88 | iodoacetamide | 1 mM, 58% residual activity | Acinetobacter calcoaceticus | |
4.1.1.88 | N-ethylmaleimide | 1 mM, 1% residual activity | Acinetobacter calcoaceticus | |
4.1.1.88 | Sodium borohydride | inactivation | Acinetobacter calcoaceticus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.1.1.88 | 1.4 | - |
malonate | pH 6.8, 30°C | Acinetobacter calcoaceticus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
4.1.1.88 | periplasm | - |
Acinetobacter calcoaceticus | - |
- |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
4.1.1.88 | 25000 | - |
2 * 65000, alpha subunit, 1 * 32000, beta subunit, 1 * 25000, gamma subunit | Acinetobacter calcoaceticus |
4.1.1.88 | 32000 | - |
2 * 65000, alpha subunit, 1 * 32000, beta subunit, 1 * 25000, gamma subunit | Acinetobacter calcoaceticus |
4.1.1.88 | 65000 | - |
2 * 65000, alpha subunit, 1 * 32000, beta subunit, 1 * 25000, gamma subunit | Acinetobacter calcoaceticus |
4.1.1.88 | 180000 | - |
PAGE | Acinetobacter calcoaceticus |
4.1.1.88 | 185000 | - |
gel filtration | Acinetobacter calcoaceticus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.1.1.88 | Acinetobacter calcoaceticus | - |
growth on malonate | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.1.1.88 | - |
Acinetobacter calcoaceticus |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
4.1.1.88 | malonate + H+ + Na+[side 1] = acetate + CO2 + Na+[side 2] | malonate decarboxylation proceeds by a catalytic cycle in which the acetyl group on the active enzyme is displaced by malonate, which binds covalently to a thiol group on the enzyme and is subsequently decarboxylated | Acinetobacter calcoaceticus |
EC Number | Renatured (Comment) | Organism |
---|---|---|
4.1.1.88 | reactivation of deacetylated enzyme by incubation with acetic anhydride or malonyl-CoA | Acinetobacter calcoaceticus |
EC Number | Storage Stability | Organism |
---|---|---|
4.1.1.88 | -20°C, stable for several months | Acinetobacter calcoaceticus |
4.1.1.88 | 4°C, deacetylated enzyme, full reactivation by addition of malonyl-CoA during 2 months storage | Acinetobacter calcoaceticus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.1.88 | 2-ethylmalonate + H+ | 0.8% of the activity with malonate | Acinetobacter calcoaceticus | butanoate + CO2 | - |
? | |
4.1.1.88 | 2-methylmalonate + H+ | 24% of the activity with malonate | Acinetobacter calcoaceticus | propionate + CO2 | - |
? | |
4.1.1.88 | glutarate + H+ | 1.1% of the activity with malonate | Acinetobacter calcoaceticus | butanoate + CO2 | - |
? | |
4.1.1.88 | malonate + H+ | enzyme is highly specific for malonate | Acinetobacter calcoaceticus | acetate + CO2 | - |
? | |
4.1.1.88 | oxalate + H+ | 1.1% of the activity with malonate | Acinetobacter calcoaceticus | CO2 | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.1.1.88 | multimer | 2 * 65000, alpha subunit, 1 * 32000, beta subunit, 1 * 25000, gamma subunit | Acinetobacter calcoaceticus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.1.1.88 | 6.8 | - |
- |
Acinetobacter calcoaceticus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
4.1.1.88 | additional information | the alpha subunit contains a biotin prosthetic group which does not seem to be directly related to malonate decarboxylation | Acinetobacter calcoaceticus |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
4.1.1.88 | Acinetobacter calcoaceticus | isoelectric focusing | - |
6.4 |