BRENDA - Enzyme Database

Purification and properties of a novel type of malonate decarboxylase from Acinetobacter calcoaceticus

Kim, Y.S.; Byun, H.S.; J. Biol. Chem. 269, 29636-29641 (1994)

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
4.1.1.88
enzyme is sensitive to repeated freezing and thawing
Acinetobacter calcoaceticus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.1.88
2-Nitro-5-thiocyanobenzoic acid
0.64 mM, 2.1% residual activity
Acinetobacter calcoaceticus
4.1.1.88
5,5'-dithio-bis(2-nitrobenzoic acid)
0.5 mM, 14.5% residual activity
Acinetobacter calcoaceticus
4.1.1.88
iodoacetamide
1 mM, 58% residual activity
Acinetobacter calcoaceticus
4.1.1.88
N-ethylmaleimide
1 mM, 1% residual activity
Acinetobacter calcoaceticus
4.1.1.88
Sodium borohydride
inactivation
Acinetobacter calcoaceticus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.1.88
1.4
-
malonate
pH 6.8, 30C
Acinetobacter calcoaceticus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
4.1.1.88
periplasm
-
Acinetobacter calcoaceticus
-
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.1.1.88
25000
-
2 * 65000, alpha subunit, 1 * 32000, beta subunit, 1 * 25000, gamma subunit
Acinetobacter calcoaceticus
4.1.1.88
32000
-
2 * 65000, alpha subunit, 1 * 32000, beta subunit, 1 * 25000, gamma subunit
Acinetobacter calcoaceticus
4.1.1.88
65000
-
2 * 65000, alpha subunit, 1 * 32000, beta subunit, 1 * 25000, gamma subunit
Acinetobacter calcoaceticus
4.1.1.88
180000
-
PAGE
Acinetobacter calcoaceticus
4.1.1.88
185000
-
gel filtration
Acinetobacter calcoaceticus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.1.1.88
Acinetobacter calcoaceticus
-
growth on malonate
-
Purification (Commentary)
EC Number
Commentary
Organism
4.1.1.88
-
Acinetobacter calcoaceticus
Reaction
EC Number
Reaction
Commentary
Organism
4.1.1.88
malonate + H+ + Na+[side 1] = acetate + CO2 + Na+[side 2]
malonate decarboxylation proceeds by a catalytic cycle in which the acetyl group on the active enzyme is displaced by malonate, which binds covalently to a thiol group on the enzyme and is subsequently decarboxylated
Acinetobacter calcoaceticus
Renatured (Commentary)
EC Number
Commentary
Organism
4.1.1.88
reactivation of deacetylated enzyme by incubation with acetic anhydride or malonyl-CoA
Acinetobacter calcoaceticus
Storage Stability
EC Number
Storage Stability
Organism
4.1.1.88
-20C, stable for several months
Acinetobacter calcoaceticus
4.1.1.88
4C, deacetylated enzyme, full reactivation by addition of malonyl-CoA during 2 months storage
Acinetobacter calcoaceticus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.1.88
2-ethylmalonate + H+
0.8% of the activity with malonate
687489
Acinetobacter calcoaceticus
butanoate + CO2
-
-
-
?
4.1.1.88
2-methylmalonate + H+
24% of the activity with malonate
687489
Acinetobacter calcoaceticus
propionate + CO2
-
-
-
?
4.1.1.88
glutarate + H+
1.1% of the activity with malonate
687489
Acinetobacter calcoaceticus
butanoate + CO2
-
-
-
?
4.1.1.88
malonate + H+
enzyme is highly specific for malonate
687489
Acinetobacter calcoaceticus
acetate + CO2
-
-
-
?
4.1.1.88
oxalate + H+
1.1% of the activity with malonate
687489
Acinetobacter calcoaceticus
CO2
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
4.1.1.88
multimer
2 * 65000, alpha subunit, 1 * 32000, beta subunit, 1 * 25000, gamma subunit
Acinetobacter calcoaceticus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.1.1.88
6.8
-
-
Acinetobacter calcoaceticus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
4.1.1.88
additional information
the alpha subunit contains a biotin prosthetic group which does not seem to be directly related to malonate decarboxylation
Acinetobacter calcoaceticus
pI Value
EC Number
Organism
Commentary
pI Value Maximum
pI Value
4.1.1.88
Acinetobacter calcoaceticus
isoelectric focusing
-
6.4
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
4.1.1.88
additional information
the alpha subunit contains a biotin prosthetic group which does not seem to be directly related to malonate decarboxylation
Acinetobacter calcoaceticus
General Stability (protein specific)
EC Number
General Stability
Organism
4.1.1.88
enzyme is sensitive to repeated freezing and thawing
Acinetobacter calcoaceticus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.1.88
2-Nitro-5-thiocyanobenzoic acid
0.64 mM, 2.1% residual activity
Acinetobacter calcoaceticus
4.1.1.88
5,5'-dithio-bis(2-nitrobenzoic acid)
0.5 mM, 14.5% residual activity
Acinetobacter calcoaceticus
4.1.1.88
iodoacetamide
1 mM, 58% residual activity
Acinetobacter calcoaceticus
4.1.1.88
N-ethylmaleimide
1 mM, 1% residual activity
Acinetobacter calcoaceticus
4.1.1.88
Sodium borohydride
inactivation
Acinetobacter calcoaceticus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.1.88
1.4
-
malonate
pH 6.8, 30C
Acinetobacter calcoaceticus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
4.1.1.88
periplasm
-
Acinetobacter calcoaceticus
-
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.1.1.88
25000
-
2 * 65000, alpha subunit, 1 * 32000, beta subunit, 1 * 25000, gamma subunit
Acinetobacter calcoaceticus
4.1.1.88
32000
-
2 * 65000, alpha subunit, 1 * 32000, beta subunit, 1 * 25000, gamma subunit
Acinetobacter calcoaceticus
4.1.1.88
65000
-
2 * 65000, alpha subunit, 1 * 32000, beta subunit, 1 * 25000, gamma subunit
Acinetobacter calcoaceticus
4.1.1.88
180000
-
PAGE
Acinetobacter calcoaceticus
4.1.1.88
185000
-
gel filtration
Acinetobacter calcoaceticus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.1.1.88
-
Acinetobacter calcoaceticus
Renatured (Commentary) (protein specific)
EC Number
Commentary
Organism
4.1.1.88
reactivation of deacetylated enzyme by incubation with acetic anhydride or malonyl-CoA
Acinetobacter calcoaceticus
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
4.1.1.88
-20C, stable for several months
Acinetobacter calcoaceticus
4.1.1.88
4C, deacetylated enzyme, full reactivation by addition of malonyl-CoA during 2 months storage
Acinetobacter calcoaceticus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.1.88
2-ethylmalonate + H+
0.8% of the activity with malonate
687489
Acinetobacter calcoaceticus
butanoate + CO2
-
-
-
?
4.1.1.88
2-methylmalonate + H+
24% of the activity with malonate
687489
Acinetobacter calcoaceticus
propionate + CO2
-
-
-
?
4.1.1.88
glutarate + H+
1.1% of the activity with malonate
687489
Acinetobacter calcoaceticus
butanoate + CO2
-
-
-
?
4.1.1.88
malonate + H+
enzyme is highly specific for malonate
687489
Acinetobacter calcoaceticus
acetate + CO2
-
-
-
?
4.1.1.88
oxalate + H+
1.1% of the activity with malonate
687489
Acinetobacter calcoaceticus
CO2
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.1.1.88
multimer
2 * 65000, alpha subunit, 1 * 32000, beta subunit, 1 * 25000, gamma subunit
Acinetobacter calcoaceticus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.1.1.88
6.8
-
-
Acinetobacter calcoaceticus
pI Value (protein specific)
EC Number
Organism
Commentary
pI Value Maximum
pI Value
4.1.1.88
Acinetobacter calcoaceticus
isoelectric focusing
-
6.4