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Literature summary extracted from

  • Wang, Z.; Wang, F.; Duan, R.; Liu, J.Y.
    Purification and physicochemical characterization of a recombinant phospholipid hydroperoxide glutathione peroxidase from Oryza sativa (2007), J. Biochem. Mol. Biol., 40, 412-418.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.11.1.12 the entire encoding region for Oryza sativa PHGPx is expressed in Escherichia coli M15 Oryza sativa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.12 0.00014
-
 1-palmitoyl-2-(13-hydroperoxy-cis-9,trans-11-octadecadienoyl)-L-3-phosphatidylcholine cosubstrate: glutathione Oryza sativa
1.11.1.12 0.0362
-
 1-palmitoyl-2-(13-hydroperoxy-cis-9,trans-11-octadecadienoyl)-L-3-phosphatidylcholine cosubstrate: thioredoxin Oryza sativa
1.11.1.12 0.799
-
 H2O2 cosubstrate: glutathione Oryza sativa
1.11.1.12 4.344
-
 tert-butyl hydroperoxide cosubstrate: glutathione Oryza sativa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.1.12 21000
-
 x * 21000, recombinant enzyme containing a six-histidine tag at its N-terminus, SDS-PAGE Oryza sativa

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.12 Oryza sativa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.12
-
 Oryza sativa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.12 glutathione + 1-palmitoyl-2-(13-hydroperoxy-cis-9,trans-11-octadecadienoyl)-L-3-phosphatidylcholine
-
 Oryza sativa ?
-
 ?
1.11.1.12 glutathione + H2O2
-
 Oryza sativa ?
-
 ?
1.11.1.12 glutathione + tert-butyl hydroperoxide
-
 Oryza sativa ?
-
 ?
1.11.1.12 thioredoxin + 1-palmitoyl-2-(13-hydroperoxy-cis-9,trans-11-octadecadienoyl)-L-3-phosphatidylcholine
-
 Oryza sativa ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.11.1.12 ? x * 21000, recombinant enzyme containing a six-histidine tag at its N-terminus, SDS-PAGE Oryza sativa

Synonyms

EC Number Synonyms Comment Organism
1.11.1.12 OsPHGPx
-
 Oryza sativa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.11.1.12 27
-
-
 Oryza sativa

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.11.1.12 10 40 10°C: 80% of maximal activity, 40°C: 60% of maximal activity Oryza sativa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.1.12 9.3
-
-
 Oryza sativa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.11.1.12 8 11 pH 8.0: about 40% of maximal activity, pH 11: about 80% of maximal activity Oryza sativa

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.11.1.12 Oryza sativa isoelectric focusing electrophoresis
-
 8.5