BRENDA - Enzyme Database

Subunit organization of bacterial malonate decarboxylases: The smallest delta subunit as an acyl-carrier protein

Byun, H.S.; Kim, Y.S.; J. Biochem. Mol. Biol. 30, 132-137 (1997)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
4.1.1.88
diethyldicarbonate
1 mM, 114% of initial activity
Pseudomonas fluorescens
4.1.1.88
N-Acetylimidazole
1 mM, 117% of initial activity
Pseudomonas fluorescens
4.1.1.88
pyridoxal 5'phosphate
1 mM, 112% of initial activity
Pseudomonas fluorescens
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.1.88
5,5'-dithio-bis(2-nitrobenzoic acid)
0.5 mM, 1.5% residual activity
Acinetobacter calcoaceticus
4.1.1.88
Bromoacetate
0.5 mM, 0.9% residual activity
Acinetobacter calcoaceticus
4.1.1.88
Bromoacetate
0.5 mM, 0.1% residual activity; 0.5 mM, 0.4% residual activity
Pseudomonas fluorescens
4.1.1.88
diethyldicarbonate
1 mM, 91% residual activity
Acinetobacter calcoaceticus
4.1.1.88
diethyldicarbonate
1 mM, 88% residual activity
Pseudomonas fluorescens
4.1.1.88
iodoacetamide
0.5 mM, 58% residual activity
Acinetobacter calcoaceticus
4.1.1.88
N-Acetylimidazole
1 mM, 95% residual activity
Acinetobacter calcoaceticus
4.1.1.88
N-Acetylimidazole
1 mM, 95% residual activity
Pseudomonas fluorescens
4.1.1.88
N-ethylmaleimide
0.5 mM, 0.1% residual activity
Acinetobacter calcoaceticus
4.1.1.88
N-ethylmaleimide
0.5 mM, 0.2% residual activity
Pseudomonas fluorescens
4.1.1.88
pyridoxal 5'phosphate
1 mM, 82% residual activity
Acinetobacter calcoaceticus
4.1.1.88
pyridoxal 5'phosphate
1 mM, 92% residual activity
Pseudomonas fluorescens
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.1.1.88
11000
-
2 * 65000, alpha subunit, 1 * 32000, beta subunit, 1 * 25000, gamma subunit, 1 * 11000, delta subunit
Acinetobacter calcoaceticus
4.1.1.88
11000
-
2 * 65000, alpha subunit, 1 * 33000, beta subunit, 1 * 30000, gamma subunit, 1 * 11000, delta subunit
Pseudomonas fluorescens
4.1.1.88
25000
-
2 * 65000, alpha subunit, 1 * 32000, beta subunit, 1 * 25000, gamma subunit, 1 * 11000, delta subunit
Acinetobacter calcoaceticus
4.1.1.88
30000
-
2 * 65000, alpha subunit, 1 * 33000, beta subunit, 1 * 30000, gamma subunit, 1 * 11000, delta subunit
Pseudomonas fluorescens
4.1.1.88
32000
-
2 * 65000, alpha subunit, 1 * 32000, beta subunit, 1 * 25000, gamma subunit, 1 * 11000, delta subunit
Acinetobacter calcoaceticus
4.1.1.88
33000
-
2 * 65000, alpha subunit, 1 * 33000, beta subunit, 1 * 30000, gamma subunit, 1 * 11000, delta subunit
Pseudomonas fluorescens
4.1.1.88
65000
-
2 * 65000, alpha subunit, 1 * 32000, beta subunit, 1 * 25000, gamma subunit, 1 * 11000, delta subunit
Acinetobacter calcoaceticus
4.1.1.88
65000
-
2 * 65000, alpha subunit, 1 * 33000, beta subunit, 1 * 30000, gamma subunit, 1 * 11000, delta subunit
Pseudomonas fluorescens
4.1.1.88
185000
-
gel filtration
Acinetobacter calcoaceticus
4.1.1.88
185000
-
gel filtration
Pseudomonas fluorescens
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.1.1.88
Acinetobacter calcoaceticus
-
grown on malonate
-
4.1.1.88
Pseudomonas fluorescens
-
grown on malonate
-
Posttranslational Modification
EC Number
Posttranslational Modification
Commentary
Organism
4.1.1.88
additional information
the delta subunit of the active form of enzyme is acetylated, the acetyl group may form a thioester bond with the thiol group of the prosthetic group
Acinetobacter calcoaceticus
4.1.1.88
additional information
the delta subunit of the active form of enzyme is acetylated, the acetyl group may form a thioester bond with the thiol group of the prosthetic group
Pseudomonas fluorescens
Purification (Commentary)
EC Number
Commentary
Organism
4.1.1.88
-
Pseudomonas fluorescens
4.1.1.88
-
Acinetobacter calcoaceticus
Renatured (Commentary)
EC Number
Commentary
Organism
4.1.1.88
reactivation of deacetyl malonate decarboxylase with malonyl-CoA, acetyl-CoA or methylmalonyl-CoA to more than 80% of initial activity
Acinetobacter calcoaceticus
4.1.1.88
reactivation of deacetyl malonate decarboxylase with malonyl-CoA, acetyl-CoA or methylmalonyl-CoA to more than 80% of initial activity; reactivation of deacetyl malonate decarboxylase with malonyl-CoA to 100%, with acetyl-CoA or methylmalonyl-CoA to about 50% of initial activity, respectively
Pseudomonas fluorescens
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.1.1.88
4.48
-
30°C
Pseudomonas fluorescens
4.1.1.88
10.02
-
30°C
Acinetobacter calcoaceticus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.1.88
malonate + H+
-
687464
Pseudomonas fluorescens
acetate + CO2
-
-
-
?
4.1.1.88
malonate + H+
-
687464
Acinetobacter calcoaceticus
acetate + CO2
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
4.1.1.88
multimer
2 * 65000, alpha subunit, 1 * 32000, beta subunit, 1 * 25000, gamma subunit, 1 * 11000, delta subunit
Acinetobacter calcoaceticus
4.1.1.88
multimer
2 * 65000, alpha subunit, 1 * 33000, beta subunit, 1 * 30000, gamma subunit, 1 * 11000, delta subunit
Pseudomonas fluorescens
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
4.1.1.88
diethyldicarbonate
1 mM, 114% of initial activity
Pseudomonas fluorescens
4.1.1.88
N-Acetylimidazole
1 mM, 117% of initial activity
Pseudomonas fluorescens
4.1.1.88
pyridoxal 5'phosphate
1 mM, 112% of initial activity
Pseudomonas fluorescens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.1.88
5,5'-dithio-bis(2-nitrobenzoic acid)
0.5 mM, 1.5% residual activity
Acinetobacter calcoaceticus
4.1.1.88
Bromoacetate
0.5 mM, 0.9% residual activity
Acinetobacter calcoaceticus
4.1.1.88
Bromoacetate
0.5 mM, 0.1% residual activity; 0.5 mM, 0.4% residual activity
Pseudomonas fluorescens
4.1.1.88
diethyldicarbonate
1 mM, 91% residual activity
Acinetobacter calcoaceticus
4.1.1.88
diethyldicarbonate
1 mM, 88% residual activity
Pseudomonas fluorescens
4.1.1.88
iodoacetamide
0.5 mM, 58% residual activity
Acinetobacter calcoaceticus
4.1.1.88
N-Acetylimidazole
1 mM, 95% residual activity
Acinetobacter calcoaceticus
4.1.1.88
N-Acetylimidazole
1 mM, 95% residual activity
Pseudomonas fluorescens
4.1.1.88
N-ethylmaleimide
0.5 mM, 0.1% residual activity
Acinetobacter calcoaceticus
4.1.1.88
N-ethylmaleimide
0.5 mM, 0.2% residual activity
Pseudomonas fluorescens
4.1.1.88
pyridoxal 5'phosphate
1 mM, 82% residual activity
Acinetobacter calcoaceticus
4.1.1.88
pyridoxal 5'phosphate
1 mM, 92% residual activity
Pseudomonas fluorescens
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.1.1.88
11000
-
2 * 65000, alpha subunit, 1 * 32000, beta subunit, 1 * 25000, gamma subunit, 1 * 11000, delta subunit
Acinetobacter calcoaceticus
4.1.1.88
11000
-
2 * 65000, alpha subunit, 1 * 33000, beta subunit, 1 * 30000, gamma subunit, 1 * 11000, delta subunit
Pseudomonas fluorescens
4.1.1.88
25000
-
2 * 65000, alpha subunit, 1 * 32000, beta subunit, 1 * 25000, gamma subunit, 1 * 11000, delta subunit
Acinetobacter calcoaceticus
4.1.1.88
30000
-
2 * 65000, alpha subunit, 1 * 33000, beta subunit, 1 * 30000, gamma subunit, 1 * 11000, delta subunit
Pseudomonas fluorescens
4.1.1.88
32000
-
2 * 65000, alpha subunit, 1 * 32000, beta subunit, 1 * 25000, gamma subunit, 1 * 11000, delta subunit
Acinetobacter calcoaceticus
4.1.1.88
33000
-
2 * 65000, alpha subunit, 1 * 33000, beta subunit, 1 * 30000, gamma subunit, 1 * 11000, delta subunit
Pseudomonas fluorescens
4.1.1.88
65000
-
2 * 65000, alpha subunit, 1 * 32000, beta subunit, 1 * 25000, gamma subunit, 1 * 11000, delta subunit
Acinetobacter calcoaceticus
4.1.1.88
65000
-
2 * 65000, alpha subunit, 1 * 33000, beta subunit, 1 * 30000, gamma subunit, 1 * 11000, delta subunit
Pseudomonas fluorescens
4.1.1.88
185000
-
gel filtration
Acinetobacter calcoaceticus
4.1.1.88
185000
-
gel filtration
Pseudomonas fluorescens
Posttranslational Modification (protein specific)
EC Number
Posttranslational Modification
Commentary
Organism
4.1.1.88
additional information
the delta subunit of the active form of enzyme is acetylated, the acetyl group may form a thioester bond with the thiol group of the prosthetic group
Acinetobacter calcoaceticus
4.1.1.88
additional information
the delta subunit of the active form of enzyme is acetylated, the acetyl group may form a thioester bond with the thiol group of the prosthetic group
Pseudomonas fluorescens
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.1.1.88
-
Pseudomonas fluorescens
4.1.1.88
-
Acinetobacter calcoaceticus
Renatured (Commentary) (protein specific)
EC Number
Commentary
Organism
4.1.1.88
reactivation of deacetyl malonate decarboxylase with malonyl-CoA, acetyl-CoA or methylmalonyl-CoA to more than 80% of initial activity
Acinetobacter calcoaceticus
4.1.1.88
reactivation of deacetyl malonate decarboxylase with malonyl-CoA, acetyl-CoA or methylmalonyl-CoA to more than 80% of initial activity; reactivation of deacetyl malonate decarboxylase with malonyl-CoA to 100%, with acetyl-CoA or methylmalonyl-CoA to about 50% of initial activity, respectively
Pseudomonas fluorescens
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.1.1.88
4.48
-
30°C
Pseudomonas fluorescens
4.1.1.88
10.02
-
30°C
Acinetobacter calcoaceticus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.1.88
malonate + H+
-
687464
Pseudomonas fluorescens
acetate + CO2
-
-
-
?
4.1.1.88
malonate + H+
-
687464
Acinetobacter calcoaceticus
acetate + CO2
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.1.1.88
multimer
2 * 65000, alpha subunit, 1 * 32000, beta subunit, 1 * 25000, gamma subunit, 1 * 11000, delta subunit
Acinetobacter calcoaceticus
4.1.1.88
multimer
2 * 65000, alpha subunit, 1 * 33000, beta subunit, 1 * 30000, gamma subunit, 1 * 11000, delta subunit
Pseudomonas fluorescens