Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Saito, M.; Kanno, M.; Iizuka, H.; Suzuki, H.
    Kinetic studies on the role of Lys-171 and Lys-358 in the beta subunit of sarcosine oxidase from Corynebacterium sp. U-96 (2007), J. Biochem., 141, 799-815.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.3.1 mutant proteins expressed in Escherichia coli BL21(DE3) Corynebacterium sp. U-96

Protein Variants

EC Number Protein Variants Comment Organism
1.5.3.1 K171A 39% activity of the wild-type enzyme, strongly increased Km for oxygen Corynebacterium sp. U-96
1.5.3.1 K171D 32% activity of the wild-type enzyme, strongly increased Km for oxygen Corynebacterium sp. U-96
1.5.3.1 K171R 58% activity of the wild-type enzyme Corynebacterium sp. U-96
1.5.3.1 K358A inactive Corynebacterium sp. U-96
1.5.3.1 K358D inactive Corynebacterium sp. U-96
1.5.3.1 K358R 0.07% of activity, increased Km Corynebacterium sp. U-96

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.3.1 0.0312
-
 oxygen K171R mutant enzyme, pH 8.0, 25°C Corynebacterium sp. U-96
1.5.3.1 0.0329
-
 oxygen wild-type enzyme, pH 8.0, 25°C Corynebacterium sp. U-96
1.5.3.1 0.386
-
 oxygen K171A mutant enzyme, pH 8.0, 25°C Corynebacterium sp. U-96
1.5.3.1 0.73
-
 oxygen K171D mutant enzyme, pH 8.0, 25°C Corynebacterium sp. U-96
1.5.3.1 1.47
-
 sarcosine K171A mutant enzyme, pH 8.0, 25°C Corynebacterium sp. U-96
1.5.3.1 1.72
-
 sarcosine K171R mutant enzyme, pH 8.0, 25°C Corynebacterium sp. U-96
1.5.3.1 2.75
-
 sarcosine K171D mutant enzyme, pH 8.0, 25°C Corynebacterium sp. U-96
1.5.3.1 2.76
-
 sarcosine wild-type enzyme, pH 8.0, 25°C Corynebacterium sp. U-96
1.5.3.1 17.4
-
 sarcosine K358R mutant enzyme, pH 8.0, 25°C Corynebacterium sp. U-96

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.3.1 sarcosine + O2 + H2O Corynebacterium sp. U-96
-
 formaldehyde + glycine + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.3.1 Corynebacterium sp. U-96
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.5.3.1 sarcosine + H2O + O2 = glycine + formaldehyde + H2O2 mechanism suggested Corynebacterium sp. U-96
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.3.1 sarcosine + O2 + H2O
-
 Corynebacterium sp. U-96 formaldehyde + glycine + H2O2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.5.3.1 monomeric sarcosine oxidase
-
 Corynebacterium sp. U-96
1.5.3.1 MSOX
-
 Corynebacterium sp. U-96

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5.3.1 0.0113
-
 sarcosine K358R mutant enzyme, pH 8.0, 25°C Corynebacterium sp. U-96
1.5.3.1 2.25
-
 sarcosine K171A mutant enzyme, pH 8.0, 25°C Corynebacterium sp. U-96
1.5.3.1 2.64
-
 sarcosine K171D mutant enzyme, pH 8.0, 25°C Corynebacterium sp. U-96
1.5.3.1 6.1
-
 oxygen K171D mutant enzyme, pH 8.0, 25°C Corynebacterium sp. U-96
1.5.3.1 7.4
-
 oxygen K171A mutant enzyme, pH 8.0, 25°C Corynebacterium sp. U-96
1.5.3.1 9.23
-
 sarcosine K171R mutant enzyme, pH 8.0, 25°C Corynebacterium sp. U-96
1.5.3.1 9.8
-
 oxygen K171R mutant enzyme, pH 8.0, 25°C Corynebacterium sp. U-96
1.5.3.1 18.5
-
 sarcosine wild-type enzyme, pH 8.0, 25°C Corynebacterium sp. U-96
1.5.3.1 18.9
-
 oxygen wild-type enzyme, pH 8.0, 25°C Corynebacterium sp. U-96

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.3.1 FAD covalent and non-covalent Corynebacterium sp. U-96
1.5.3.1 FMN covalent Corynebacterium sp. U-96