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Literature summary extracted from

  • Nobre, A.; Alarico, S.; Fernandes, C.; Empadinhas, N.; da Costa, M.S.
    A unique combination of genetic systems for the synthesis of trehalose in Rubrobacter xylanophilus: properties of a rare actinobacterial TreT (2008), J. Bacteriol., 190, 7939-7946.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.245 expressed in Escherichia coli BL21(DE3) cells Rubrobacter xylanophilus
3.1.3.12 expressed in Escherichia coli as a His-tagged fusion protein Rubrobacter xylanophilus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.245 Ni2+ 2 mM Rubrobacter xylanophilus
2.4.1.245 Zn2+ 2 mM Rubrobacter xylanophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.245 0.8
-
ADP-glucose recombinant enzyme, at 60°C Rubrobacter xylanophilus
2.4.1.245 1.3
-
D-glucose recombinant enzyme, at 60°C Rubrobacter xylanophilus
2.4.1.245 6.8
-
ADP recombinant enzyme, at 60°C Rubrobacter xylanophilus
2.4.1.245 82
-
alpha,alpha-1,1-trehalose recombinant enzyme, at 60°C Rubrobacter xylanophilus
2.4.1.347 0.7
-
GDP-glucose 37°C Rubrobacter xylanophilus
2.4.1.347 2.9
-
D-glucose 6-phosphate 37°C Rubrobacter xylanophilus
3.1.3.12 6.1
-
trehalose 6-phosphate Vmax: 225 microgram/min/mg protein Rubrobacter xylanophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.245 Ca2+ 2 mM or 20 mM Ca2+ induces slightly enhanced activity Rubrobacter xylanophilus
2.4.1.245 Fe3+ 2 mM Fe3+ induces slightly enhanced activity Rubrobacter xylanophilus
2.4.1.245 Li+ 2 mM or 20 mM Li+ induces slightly enhanced activity Rubrobacter xylanophilus
2.4.1.245 Mg2+ Mg2+ is required for activity, 20 mM Mg+ induces slightly enhanced activity Rubrobacter xylanophilus
2.4.1.245 Mn2+ 2 mM Mn2+ induces slightly enhanced activity Rubrobacter xylanophilus
2.4.1.245 additional information no enhanced activity in the presence of 20 mM Fe3+ or Mn2+ Rubrobacter xylanophilus
2.4.1.347 Mg2+ stimulation, maximum activity at 2 mM. In absence of Mg2+, 36% of maximum activity Rubrobacter xylanophilus
3.1.3.12 Mg2+ 2mM Mg2+ or Co2+ Rubrobacter xylanophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.245 46700
-
calculated from amino acid sequence Rubrobacter xylanophilus
2.4.1.347 53900
-
x * 53900, calculated Rubrobacter xylanophilus
3.1.3.12 29400
-
deduced from cDNA Rubrobacter xylanophilus

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.245 Rubrobacter xylanophilus Q1ARU5 strain PRD-1T (DSM 9941)
-
2.4.1.347 Rubrobacter xylanophilus B8R7Q2
-
-
3.1.3.12 Rubrobacter xylanophilus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.245 HisTrap column chromatography, Q-Sepharose fast-flow column chromatography, and Superdex 200 gel filtration Rubrobacter xylanophilus
3.1.3.12 using Ni-NTA chromatography Rubrobacter xylanophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.245 ADP-alpha-D-glucose + D-glucose 100% activity Rubrobacter xylanophilus ADP + alpha,alpha-1,1-trehalose
-
?
2.4.1.245 GDP-glucose + D-glucose 48.2% activity compared to ADP-glucose Rubrobacter xylanophilus alpha,alpha-1,1-trehalose + GDP
-
?
2.4.1.245 UDP-glucose + D-glucose 32.7% activity compared to ADP-glucose Rubrobacter xylanophilus alpha,alpha-1,1-trehalose + UDP
-
?
2.4.1.347 GDP-alpha-D-glucose + D-glucose 6-phosphate GDP-glucose is the preferred glycosyl-donor Rubrobacter xylanophilus GDP + alpha,alpha-trehalose 6-phosphate
-
?
3.1.3.12 trehalose 6-phosphate + H2O
-
Rubrobacter xylanophilus trehalose + phosphate
-
?

Subunits

EC Number Subunits Comment Organism
2.4.1.347 ? x * 53900, calculated Rubrobacter xylanophilus

Synonyms

EC Number Synonyms Comment Organism
2.4.1.245 trehalose glycosyltransferring synthase
-
Rubrobacter xylanophilus
2.4.1.245 TreT
-
Rubrobacter xylanophilus
3.1.3.12 TPP
-
Rubrobacter xylanophilus
3.1.3.12 trehalose 6-phosphate phosphatase
-
Rubrobacter xylanophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.245 60
-
-
Rubrobacter xylanophilus
2.4.1.347 60
-
-
Rubrobacter xylanophilus
3.1.3.12 60
-
assay at and optimal activity Rubrobacter xylanophilus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.1.245 20 80
-
Rubrobacter xylanophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.245 60 70 the half-lives for inactivation at 60°C and at 70°C are 309 h and 4.1 h, respectively Rubrobacter xylanophilus
2.4.1.347 60
-
half-life 1.7 h Rubrobacter xylanophilus
2.4.1.347 70
-
half-life 0.7 h Rubrobacter xylanophilus
3.1.3.12 60
-
half-life: 3.2 h Rubrobacter xylanophilus
3.1.3.12 70
-
half-life: 1.0 h Rubrobacter xylanophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.245 8 10
-
Rubrobacter xylanophilus
2.4.1.347 7
-
-
Rubrobacter xylanophilus
3.1.3.12 7
-
assay at Rubrobacter xylanophilus
3.1.3.12 7.5
-
optimal activity Rubrobacter xylanophilus