EC Number | Cloned (Comment) | Organism |
---|---|---|
2.4.1.245 | expressed in Escherichia coli BL21(DE3) cells | Rubrobacter xylanophilus |
3.1.3.12 | expressed in Escherichia coli as a His-tagged fusion protein | Rubrobacter xylanophilus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.4.1.245 | Ni2+ | 2 mM | Rubrobacter xylanophilus | |
2.4.1.245 | Zn2+ | 2 mM | Rubrobacter xylanophilus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.1.245 | 0.8 | - |
ADP-glucose | recombinant enzyme, at 60°C | Rubrobacter xylanophilus | |
2.4.1.245 | 1.3 | - |
D-glucose | recombinant enzyme, at 60°C | Rubrobacter xylanophilus | |
2.4.1.245 | 6.8 | - |
ADP | recombinant enzyme, at 60°C | Rubrobacter xylanophilus | |
2.4.1.245 | 82 | - |
alpha,alpha-1,1-trehalose | recombinant enzyme, at 60°C | Rubrobacter xylanophilus | |
2.4.1.347 | 0.7 | - |
GDP-glucose | 37°C | Rubrobacter xylanophilus | |
2.4.1.347 | 2.9 | - |
D-glucose 6-phosphate | 37°C | Rubrobacter xylanophilus | |
3.1.3.12 | 6.1 | - |
trehalose 6-phosphate | Vmax: 225 microgram/min/mg protein | Rubrobacter xylanophilus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.4.1.245 | Ca2+ | 2 mM or 20 mM Ca2+ induces slightly enhanced activity | Rubrobacter xylanophilus | |
2.4.1.245 | Fe3+ | 2 mM Fe3+ induces slightly enhanced activity | Rubrobacter xylanophilus | |
2.4.1.245 | Li+ | 2 mM or 20 mM Li+ induces slightly enhanced activity | Rubrobacter xylanophilus | |
2.4.1.245 | Mg2+ | Mg2+ is required for activity, 20 mM Mg+ induces slightly enhanced activity | Rubrobacter xylanophilus | |
2.4.1.245 | Mn2+ | 2 mM Mn2+ induces slightly enhanced activity | Rubrobacter xylanophilus | |
2.4.1.245 | additional information | no enhanced activity in the presence of 20 mM Fe3+ or Mn2+ | Rubrobacter xylanophilus | |
2.4.1.347 | Mg2+ | stimulation, maximum activity at 2 mM. In absence of Mg2+, 36% of maximum activity | Rubrobacter xylanophilus | |
3.1.3.12 | Mg2+ | 2mM Mg2+ or Co2+ | Rubrobacter xylanophilus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.4.1.245 | 46700 | - |
calculated from amino acid sequence | Rubrobacter xylanophilus |
2.4.1.347 | 53900 | - |
x * 53900, calculated | Rubrobacter xylanophilus |
3.1.3.12 | 29400 | - |
deduced from cDNA | Rubrobacter xylanophilus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.1.245 | Rubrobacter xylanophilus | Q1ARU5 | strain PRD-1T (DSM 9941) | - |
2.4.1.347 | Rubrobacter xylanophilus | B8R7Q2 | - |
- |
3.1.3.12 | Rubrobacter xylanophilus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.4.1.245 | HisTrap column chromatography, Q-Sepharose fast-flow column chromatography, and Superdex 200 gel filtration | Rubrobacter xylanophilus |
3.1.3.12 | using Ni-NTA chromatography | Rubrobacter xylanophilus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.245 | ADP-alpha-D-glucose + D-glucose | 100% activity | Rubrobacter xylanophilus | ADP + alpha,alpha-1,1-trehalose | - |
? | |
2.4.1.245 | GDP-glucose + D-glucose | 48.2% activity compared to ADP-glucose | Rubrobacter xylanophilus | alpha,alpha-1,1-trehalose + GDP | - |
? | |
2.4.1.245 | UDP-glucose + D-glucose | 32.7% activity compared to ADP-glucose | Rubrobacter xylanophilus | alpha,alpha-1,1-trehalose + UDP | - |
? | |
2.4.1.347 | GDP-alpha-D-glucose + D-glucose 6-phosphate | GDP-glucose is the preferred glycosyl-donor | Rubrobacter xylanophilus | GDP + alpha,alpha-trehalose 6-phosphate | - |
? | |
3.1.3.12 | trehalose 6-phosphate + H2O | - |
Rubrobacter xylanophilus | trehalose + phosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.4.1.347 | ? | x * 53900, calculated | Rubrobacter xylanophilus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.4.1.245 | trehalose glycosyltransferring synthase | - |
Rubrobacter xylanophilus |
2.4.1.245 | TreT | - |
Rubrobacter xylanophilus |
3.1.3.12 | TPP | - |
Rubrobacter xylanophilus |
3.1.3.12 | trehalose 6-phosphate phosphatase | - |
Rubrobacter xylanophilus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.1.245 | 60 | - |
- |
Rubrobacter xylanophilus |
2.4.1.347 | 60 | - |
- |
Rubrobacter xylanophilus |
3.1.3.12 | 60 | - |
assay at and optimal activity | Rubrobacter xylanophilus |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.1.245 | 20 | 80 | - |
Rubrobacter xylanophilus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.1.245 | 60 | 70 | the half-lives for inactivation at 60°C and at 70°C are 309 h and 4.1 h, respectively | Rubrobacter xylanophilus |
2.4.1.347 | 60 | - |
half-life 1.7 h | Rubrobacter xylanophilus |
2.4.1.347 | 70 | - |
half-life 0.7 h | Rubrobacter xylanophilus |
3.1.3.12 | 60 | - |
half-life: 3.2 h | Rubrobacter xylanophilus |
3.1.3.12 | 70 | - |
half-life: 1.0 h | Rubrobacter xylanophilus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.4.1.245 | 8 | 10 | - |
Rubrobacter xylanophilus |
2.4.1.347 | 7 | - |
- |
Rubrobacter xylanophilus |
3.1.3.12 | 7 | - |
assay at | Rubrobacter xylanophilus |
3.1.3.12 | 7.5 | - |
optimal activity | Rubrobacter xylanophilus |