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Literature summary extracted from
- ATPase activity of Mycobacterium tuberculosis SecA1 and SecA2 proteins and its importance to SecA2 function in macrophages (2008), J. Bacteriol., 190, 4880-4887.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 7.4.2.5 | - |
Escherichia coli |
| 7.4.2.5 | into the pET29a vector for expression in Escherichia coli BL21DE3 cells | Mycobacterium tuberculosis |
| 7.4.2.5 | into the pET41b vector for expression in Escherichia coli BL21DE3 cells | Mycobacterium tuberculosis |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 7.4.2.5 | K108R | mutant, defective in ATP binding and protein translocation in vitro, as well as biologically inactive in vivo | Escherichia coli |
| 7.4.2.5 | K115A | mutant, substitution of the conserved lysine in the Walker A motif eliminates ATP binding and affects the biological activity | Mycobacterium tuberculosis |
| 7.4.2.5 | K115R | mutant, substitution of the conserved lysine in the Walker A motif eliminates ATP binding and affects the biological activity | Mycobacterium tuberculosis |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 7.4.2.5 | additional information | - |
additional information | ATP hydrolysis rate 10.5 nmol phosphate/min/nmol SecA1 | Mycobacterium tuberculosis | |
| 7.4.2.5 | additional information | - |
additional information | ATP hydrolysis rate SecA wild-type 6.1 nmol phosphate/min/nmol enzyme | Escherichia coli | |
| 7.4.2.5 | additional information | - |
additional information | ATP hydrolysis rate SecA2 wild-type 19.7, K115R mutant 25.0, K115A mutant 13.5 nmol Pi/min/nmol enzyme | Mycobacterium tuberculosis |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 7.4.2.5 | cytoplasm | - |
Mycobacterium tuberculosis | 5737 | - |
| 7.4.2.5 | cytoplasm | - |
Escherichia coli | 5737 | - |
| 7.4.2.5 | cytoplasmic membrane | - |
Mycobacterium tuberculosis | - |
- |
| 7.4.2.5 | cytoplasmic membrane | - |
Escherichia coli | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 7.4.2.5 | 85400 | - |
monomer, predicted molecular mass, determined by SDS-PAGE | Mycobacterium tuberculosis |
| 7.4.2.5 | 95000 | - |
determined by SDS-PAGE | Escherichia coli |
| 7.4.2.5 | 101000 | - |
monomer, predicted molecular mass | Escherichia coli |
| 7.4.2.5 | 106000 | - |
monomer, predicted molecular mass, determined by SDS-PAGE | Mycobacterium tuberculosis |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 7.4.2.5 | ATP + H2O | Mycobacterium tuberculosis | - |
ADP + phosphate | - |
ir |  |
| 7.4.2.5 | ATP + H2O | Escherichia coli | - |
ADP + phosphate | - |
ir | |
| 7.4.2.5 | ATP + H2O | Mycobacterium tuberculosis H37Rv | - |
ADP + phosphate | - |
ir | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 7.4.2.5 | Escherichia coli | P10408 | - |
- |
| 7.4.2.5 | Mycobacterium tuberculosis | P9WGP3 | - |
- |
| 7.4.2.5 | Mycobacterium tuberculosis | P9WGP5 | - |
- |
| 7.4.2.5 | Mycobacterium tuberculosis H37Rv | P9WGP3 | - |
- |
| 7.4.2.5 | Mycobacterium tuberculosis H37Rv | P9WGP5 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 7.4.2.5 | - |
Escherichia coli |
| 7.4.2.5 | using a Blue-Sepharose CL-6B column | Mycobacterium tuberculosis |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 7.4.2.5 | ATP + H2O | - |
Mycobacterium tuberculosis | ADP + phosphate | - |
ir | |
| 7.4.2.5 | ATP + H2O | - |
Escherichia coli | ADP + phosphate | - |
ir | |
| 7.4.2.5 | ATP + H2O | - |
Mycobacterium tuberculosis H37Rv | ADP + phosphate | - |
ir | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 7.4.2.5 | SecA | - |
Escherichia coli |
| 7.4.2.5 | SecA ATPase | - |
Escherichia coli |
| 7.4.2.5 | SecA1 | - |
Mycobacterium tuberculosis |
| 7.4.2.5 | SecA1 ATPase | - |
Mycobacterium tuberculosis |
| 7.4.2.5 | SecA2 | - |
Mycobacterium tuberculosis |
| 7.4.2.5 | SecA2 ATPase | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 7.4.2.5 | 25 | - |
ATPase activity assay | Mycobacterium tuberculosis |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 7.4.2.5 | 7 | - |
ATPase activity assay | Mycobacterium tuberculosis |
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