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Literature summary extracted from

  • Anderson, P.J.; Lango, J.; Carkeet, C.; Britten, A.; Kraeutler, B.; Hammock, B.D.; Roth, J.R.
    One pathway can incorporate either adenine or dimethylbenzimidazole as an alpha-axial ligand of B12 cofactors in Salmonella enterica (2008), J. Bacteriol., 190, 1160-1171.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.156 Chromosomal in-frame deletion mutations are constructed for the cobU gene using linear transformation and moved into strains with the deletion mutation apt-18, which causes strains to synthesize only pseudo-B12 cofactors. Salmonella enterica subsp. enterica serovar Typhimurium

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.156 additional information The cobU mutations all eliminate the ability of the apt-18 deletion mutant to grow on cobinamide Salmonella enterica subsp. enterica serovar Typhimurium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.156 adenosylcobinamide phosphate + GTP Salmonella enterica subsp. enterica serovar Typhimurium
-
 adenosylcobinamide-GDP + diphosphate
-
 ?
2.7.1.156 additional information Salmonella enterica subsp. enterica serovar Typhimurium CobU enzyme is required for synthesis of both coenzyme B12 (alpha ligand: 5,6-dimethylbenzimidazole) and pseudo-coenzyme B12 (alpha ligand: adenine). ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.156 Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.1.156 RTP + adenosylcobinamide = adenosylcobinamide phosphate + RDP Aminopropanol side chain of the corrin cobinamide is activated by the addition of GDP. Synthesis of the coenzyme pseudo-B12 cofactors requires the same enzymes (e.g. CobU) which install 5,6-dimethylbenzimidazole in the formation of coenzyme B12 Salmonella enterica subsp. enterica serovar Typhimurium
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.156 cell culture strain LT2 Salmonella enterica subsp. enterica serovar Typhimurium
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.156 adenosylcobinamide phosphate + GTP
-
 Salmonella enterica subsp. enterica serovar Typhimurium adenosylcobinamide-GDP + diphosphate
-
 ?
2.7.1.156 additional information CobU enzyme is required for synthesis of both coenzyme B12 (alpha ligand: 5,6-dimethylbenzimidazole) and pseudo-coenzyme B12 (alpha ligand: adenine). Salmonella enterica subsp. enterica serovar Typhimurium ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.156 CobU
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.156 ATP
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.7.1.156 GTP
-
 Salmonella enterica subsp. enterica serovar Typhimurium