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Literature summary extracted from

  • Fukushima, E.; Shinka, Y.; Fukui, T.; Atomi, H.; Imanaka, T.
    Methionine sulfoxide reductase from the hyperthermophilic archaeon Thermococcus kodakaraensis, an enzyme designed to function at suboptimal growth temperatures (2007), J. Bacteriol., 189, 7134-7144.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.4.11 expression inm Escherichia coli, recombinant proteins corresponding to MsrABTk and the individual domains (MsrATk and MsrBTk) are produced Thermococcus kodakarensis
1.8.4.12 expression inm Escherichia coli, recombinant proteins corresponding to MsrABTk and the individual domains (MsrATk and MsrBTk) are produced Thermococcus kodakarensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.4.11 15
-
 dithiothreitol MsrA domain of MsrABTk, in the presence of 80 mM L-methionine (S)-sulfoxide Thermococcus kodakarensis
1.8.4.11 19
-
 L-methionine (S)-sulfoxide MsrA domain of MsrABTk, in the presence of 100 mM dithiothreiol Thermococcus kodakarensis
1.8.4.12 2
-
 dithiothreitol MsrB domain of MsrABTk, in the presence of 80 mM L-methionine (S)-sulfoxide Thermococcus kodakarensis
1.8.4.12 67
-
 L-methionine (R)-sulfoxide MsrB domain of MsrABTk, in the presence of 20 mM dithiothreiol Thermococcus kodakarensis

Organism

EC Number Organism UniProt Comment Textmining
1.8.4.11 Thermococcus kodakarensis
-
-
-
1.8.4.12 Thermococcus kodakarensis
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.8.4.11 additional information presence o MsrABTk is greater in Thermococcus kodakaraensis cells grown at suboptimal temperatures (60 to 70°C) and could not be detected at 80 to 90°C. The amount of intracellular MsrABTk protein increases with exposure to higher dissolved oxygen levels, but only at suboptimal growth temperatures Thermococcus kodakarensis
-
1.8.4.12 additional information presence of MsrABTk is greater in Thermococcus kodakaraensis cells grown at suboptimal temperatures (60 to 70°C) and could not be detected at 80 to 90°C. The amount of intracellular MsrABTk protein increases with exposure to higher dissolved oxygen levels, but only at suboptimal growth temperatures Thermococcus kodakarensis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.4.11 dabsyl-L-methionine-(S)-S-oxide + dithiothreitol
-
 Thermococcus kodakarensis dabsyl-L-methionine + dithiothreitol disulfide + H2O
-
 ?
1.8.4.11 L-methionine (S)-sulfoxide + 2 dithiothreitol the MsrA-domain of MsrABTk is strictly specific for the reduction of L-methionine (S)-sulfoxide Thermococcus kodakarensis L-methionine + dithiothreitol disulfide + H2O
-
 ?
1.8.4.12 dabsyl-L-methionine-(R)-S-oxide + dithiothreitol
-
 Thermococcus kodakarensis dabsyl-L-methionine + dithiothreitol disulfide + H2O
-
 ?
1.8.4.12 L-methionine (R)-sulfoxide + dithiothreitol the MsrB-domain of MsrABTk is strictly specific for the reduction of L-methionine (R)-sulfoxide Thermococcus kodakarensis L-methionine + dithiothreitol disulfide
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.8.4.11 MsrABTk bifunctional enzyme (EC 1.8.1.11/1.8.1.12) Thermococcus kodakarensis
1.8.4.12 MsrABTk bifunctional enzyme (EC 1.8.1.11/1.8.1.12) Thermococcus kodakarensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.8.4.11 60
-
 MsrA domain of MsrABTk, substrate: dabsyl-L-methionine-(S)-S-oxide Thermococcus kodakarensis
1.8.4.12 30
-
 MsrB domain of MsrABTk Thermococcus kodakarensis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.8.4.11 80
-
 half-life of MsrABTk is 30 min. Half-life of the MsrA domain within the MsrABTk protein is 27 min. The recombinant MsrA protein shows a half-life of less than 1 min. The MsrA domain is stabilized through its physical interaction with the more thermostable MsrB domain Thermococcus kodakarensis
1.8.4.11 85
-
 75% decrease in activity after 2.5 min, MsrABTk Thermococcus kodakarensis
1.8.4.12 80
-
 half-life of MsrABTk is 30 min, half-life of MsrB domain is 31 min Thermococcus kodakarensis
1.8.4.12 85
-
 75% decrease in activity after 2.5 min, MsrABTk Thermococcus kodakarensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.4.11 7.5 8 optimum pH of the MsrA domain Thermococcus kodakarensis
1.8.4.12 6 7 optimum pH of the MsrB domain Thermococcus kodakarensis