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Literature summary extracted from

  • Orita, I.; Sato, T.; Yurimoto, H.; Kato, N.; Atomi, H.; Imanaka, T.; Sakai, Y.
    The ribulose monophosphate pathway substitutes for the missing pentose phosphate pathway in the archaeon Thermococcus kodakaraensis (2006), J. Bacteriol., 188, 4698-4704.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.2.43 expressed in Escherichia coli Rosetta(DE3) cells Thermococcus kodakarensis
5.3.1.27
-
 Thermococcus kodakarensis

Protein Variants

EC Number Protein Variants Comment Organism
5.3.1.27 additional information deletion of bifunctional 3-hexulose-6-phosphate synthase /6-phospho-3-hexuloisomerase results in loss of growth in minimal medium, which can be recovered by addition of nucleosides to the medium Thermococcus kodakarensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.2.43 44000
-
 recombinant fusion enzyme HPS-PHI, SDS-PAGE Thermococcus kodakarensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.3.1.27 additional information Thermococcus kodakarensis bifunctional 3-hexulose-6-phosphate synthase/6-phospho-3-hexuloisomerase is essential for the biosynthesis of ribulose 5-phosphate. The ribulose monophosphate pathway substitutes for the classical pentose phosphate pathway in Thermococcus kodakarensis ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.43 Thermococcus kodakarensis
-
-
-
5.3.1.27 Thermococcus kodakarensis
-
 bifunctional 3-hexulose-6-phosphate synthase/6-phospho-3-hexuloisomerase
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.2.43 ultracentrifugation Thermococcus kodakarensis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.2.43 9.8
-
 cell free extract Thermococcus kodakarensis
4.1.2.43 148
-
 after 15.1fold purification Thermococcus kodakarensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.43 D-ribulose 5-phosphate + formaldehyde
-
 Thermococcus kodakarensis D-arabino-hex-3-ulose 6-phosphate
-
 ?
5.3.1.27 additional information bifunctional 3-hexulose-6-phosphate synthase/6-phospho-3-hexuloisomerase is essential for the biosynthesis of ribulose 5-phosphate. The ribulose monophosphate pathway substitutes for the classical pentose phosphate pathway in Thermococcus kodakarensis Thermococcus kodakarensis ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.1.2.43 3-hexulose-6-phosphate synthase
-
 Thermococcus kodakarensis
4.1.2.43 HPS/PHI bifunctional fusion enzyme Thermococcus kodakarensis