EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.2.5.2 | ethylamine | little ADH I activity is detected in the absence of ethylamine or ammonium ions | Pseudomonas putida | |
1.2.5.2 | additional information | ADH I is induced by ethanol | Pseudomonas putida | |
1.2.5.2 | NH4+ | little ADH I activity is detected in the absence of ethylamine or ammonium ions | Pseudomonas putida |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.2.5.2 | EDTA | allows dissociation of ADH I into two identical monomer subunits, releasing PQQ | Pseudomonas putida |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.9.1 | 0.105 | - |
1-butanol | isoform ADH IIB, pH 8.0 | Pseudomonas putida | |
1.1.9.1 | 0.15 | - |
1-butanol | isoform ADH IIG, pH 8.0 | Pseudomonas putida | |
1.1.9.1 | 0.226 | - |
1,2-propanediol | isoform ADH IIG, pH 8.0 | Pseudomonas putida | |
1.1.9.1 | 2.4 | - |
glycerol | isoform ADH IIG, pH 8.0 | Pseudomonas putida | |
1.1.9.1 | 3 | 3.2 | ethanol | isoform ADH IIG, pH 8.0 | Pseudomonas putida | |
1.1.9.1 | 5.61 | - |
ethanol | isoform ADH IIB, pH 8.0 | Pseudomonas putida | |
1.1.9.1 | 10.2 | - |
1,2-propanediol | isoform ADH IIB, pH 8.0 | Pseudomonas putida |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.2.5.2 | periplasm | - |
Pseudomonas putida | - |
- |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.9.1 | 69000 | - |
ADH IIB, 1 * 69000, SDS-PAGE. ADH IIG, 1 * 72000, SDS-PAGE | Pseudomonas putida |
1.1.9.1 | 72000 | - |
ADH IIB, 1 * 69000, SDS-PAGE. ADH IIG, 1 * 72000, SDS-PAGE | Pseudomonas putida |
1.2.5.2 | 63000 | - |
ADH IIB, gel filtration | Pseudomonas putida |
1.2.5.2 | 64000 | - |
2 * 64000, ADH I, SDS-PAGE | Pseudomonas putida |
1.2.5.2 | 68000 | - |
ADH IIG, gel filtration | Pseudomonas putida |
1.2.5.2 | 69000 | - |
1 * 69000, ADH IIB | Pseudomonas putida |
1.2.5.2 | 72000 | - |
1 * 72000, ADH IIG | Pseudomonas putida |
1.2.5.2 | 130000 | - |
ADH I, gel filtration | Pseudomonas putida |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.5.2 | additional information | Pseudomonas putida | three distinct quinoprotein ADHs (ADHs, ADH IIB and ADH IIG) are synthesized by the bacterium under different growth conditions. ADH I is formed most abundantly in the cells grown on ethanol | ? | - |
? | |
1.2.5.2 | additional information | Pseudomonas putida | three distinct quinoprotein ADHs (ADHs, ADH IIB and ADH IIG) are synthesized by the bacterium under different growth conditions. ADH IIB is induced by n-butanol, 1,3-butandiol, and alcohols of medium chain length | ? | - |
? | |
1.2.5.2 | additional information | Pseudomonas putida | three distinct quinoprotein ADHs (ADHs, ADH IIB and ADH IIG) are synthesized by the bacterium under different growth conditions. Induction of ADH IIG is restricted to 1,2-propanediol or glycerol | ? | - |
? | |
1.2.5.2 | additional information | Pseudomonas putida HK5 | three distinct quinoprotein ADHs (ADHs, ADH IIB and ADH IIG) are synthesized by the bacterium under different growth conditions. ADH I is formed most abundantly in the cells grown on ethanol | ? | - |
? | |
1.2.5.2 | additional information | Pseudomonas putida HK5 | three distinct quinoprotein ADHs (ADHs, ADH IIB and ADH IIG) are synthesized by the bacterium under different growth conditions. Induction of ADH IIG is restricted to 1,2-propanediol or glycerol | ? | - |
? | |
1.2.5.2 | additional information | Pseudomonas putida HK5 | three distinct quinoprotein ADHs (ADHs, ADH IIB and ADH IIG) are synthesized by the bacterium under different growth conditions. ADH IIB is induced by n-butanol, 1,3-butandiol, and alcohols of medium chain length | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.9.1 | Pseudomonas putida | - |
- |
- |
1.2.5.2 | Pseudomonas putida | - |
isozyme ADH I | - |
1.2.5.2 | Pseudomonas putida | Q4W6G0 | ADH IIG; isozyme ADH IIG | - |
1.2.5.2 | Pseudomonas putida | Q8GR64 | isozyme ADH IIB | - |
1.2.5.2 | Pseudomonas putida HK5 | - |
isozyme ADH I | - |
1.2.5.2 | Pseudomonas putida HK5 | Q4W6G0 | ADH IIG; isozyme ADH IIG | - |
1.2.5.2 | Pseudomonas putida HK5 | Q8GR64 | isozyme ADH IIB | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.2.5.2 | native isozyme ADH I, a procedure using anion exchange and hydrophobic interaction chromatographies with elution buffers containing 50% glycerol or PEG 600, ultrafiltration, and gel filtration, overview | Pseudomonas putida |
1.2.5.2 | native isozyme ADH IIB, a procedure using anion exchange and hydrophobic interaction chromatographies with elution buffers containing 50% glycerol or PEG 600, ultrafiltration, and gel filtration, overview | Pseudomonas putida |
1.2.5.2 | native isozyme ADH IIG, a procedure using anion exchange and hydrophobic interaction chromatographies with elution buffers containing 50% glycerol or PEG 600, ultrafiltration, and gel filtration, overview | Pseudomonas putida |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.2.5.2 | culture condition:1,2-propanediol-grown cell | isozyme ADH IIG | Pseudomonas putida | - |
1.2.5.2 | culture condition:ethanol-grown cell | isozyme ADH I | Pseudomonas putida | - |
1.2.5.2 | culture condition:n-butanol-grown cell | isozyme ADH IIB | Pseudomonas putida | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.9.1 | 14 | - |
isoform ADH IIG, pH 9.0 | Pseudomonas putida |
1.2.5.2 | additional information | - |
substrate specificities of ADH isozymes, detailed overview | Pseudomonas putida |
1.2.5.2 | 14 | - |
purified ADH IIG, substrate glycerol with cofactor ferricyanide | Pseudomonas putida |
1.2.5.2 | 26.8 | - |
purified ADH I, substrate ethanol with cofactors | Pseudomonas putida |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.9.1 | 1,2-butanediol + ferricyanide | ADH IIB, 84% of the activity with 1-butanol. ADH IIG, 86% of the activity with 1,2-propanediol | Pseudomonas putida | 2-hydroxybutanal + ferrocyanide | - |
? | |
1.1.9.1 | 1,2-ethanediol + ferricyanide | ADH IIB, 0% of the activity with 1-butanol. ADH IIG, 71% of the activity with 1,2-propanediol | Pseudomonas putida | hydroxyacetaldehyde + ferrocyanide | - |
? | |
1.1.9.1 | 1,2-propanediol + ferricyanide | ADH IIB, 43% of the activity with 1-butanol. ADH IIG, maximum activity | Pseudomonas putida | 2-hydroxypropanal + ferrocyanide | - |
? | |
1.1.9.1 | 1,3-butanediol + ferricyanide | ADH IIB, 105% of the activity with 1-butanol. ADH IIG, 75% of the activity with 1,2-propanediol | Pseudomonas putida | 3-hydroxybutanal + ferrocyanide | - |
? | |
1.1.9.1 | 1,3-propandione + ferricyanide | ADH IIB, 61% of the activity with 1-butanol. ADH IIG, 42% of the activity with 1,2-propanediol | Pseudomonas putida | 3-hydroxypropanal + ferrocyanide | - |
? | |
1.1.9.1 | 1,4-butanediol + ferricyanide | ADH IIB, 110% of the activity with 1-butanol. ADH IIG, 75% of the activity with 1,2-propanediol | Pseudomonas putida | 4-hydroxybutanal + ferrocyanide | - |
? | |
1.1.9.1 | 1-butanol + ferricyanide | ADH IIG, 42% of the activity with 1,2-propanediol | Pseudomonas putida | butanal + ferrocyanide | - |
? | |
1.1.9.1 | 1-heptanol + ferricyanide | ADH IIB, 99% of the activity with 1-butanol. ADH IIG, 39% of the activity with 1,2-propanediol | Pseudomonas putida | heptanal + ferrocyanide | - |
? | |
1.1.9.1 | 1-hexanol + ferricyanide | ADH IIB, 70% of the activity with 1-butanol. ADH IIG, 46% of the activity with 1,2-propanediol | Pseudomonas putida | hexanal + ferrocyanide | - |
? | |
1.1.9.1 | 1-octanol + ferricyanide | ADH IIB, 44% of the activity with 1-butanol. ADH IIG, 24% of the activity with 1,2-propanediol | Pseudomonas putida | octanal + ferrocyanide | - |
? | |
1.1.9.1 | 1-pentanol + ferricyanide | ADH IIB, 100% of the activity with 1-butanol. ADH IIG, 40% of the activity with 1,2-propanediol | Pseudomonas putida | pentanal + ferrocyanide | - |
? | |
1.1.9.1 | 1-propanol + ferricyanide | ADH IIB, 70% of the activity with 1-butanol. ADH IIG, 41% of the activity with 1,2-propanediol | Pseudomonas putida | propanal + ferrocyanide | - |
? | |
1.1.9.1 | 2,3-butanediol + ferricyanide | ADH IIB, 12% of the activity with 1-butanol. ADH IIG, 71% of the activity with 1,2-propanediol | Pseudomonas putida | 3-hydroxybutan-2-one + ferrocyanide | - |
? | |
1.1.9.1 | 2,4-pentanediol + ferricyanide | ADH IIB, 45% of the activity with 1-butanol. ADH IIG, 0% of the activity with 1,2-propanediol | Pseudomonas putida | 4-hydroxypentan-2-one + ferrocyanide | - |
? | |
1.1.9.1 | 2-butanol + ferricyanide | ADH IIB, 90% of the activity with 1-butanol. ADH IIG, 15% of the activity with 1,2-propanediol | Pseudomonas putida | butanone + ferrocyanide | - |
? | |
1.1.9.1 | 2-hexanol + ferricyanide | ADH IIB, 99% of the activity with 1-butanol. ADH IIG, 33% of the activity with 1,2-propanediol | Pseudomonas putida | hexan-2-one + ferrocyanide | - |
? | |
1.1.9.1 | 2-pentanol + ferricyanide | ADH IIB, 120% of the activity with 1-butanol. ADH IIG, 19% of the activity with 1,2-propanediol | Pseudomonas putida | pentan-2-one + ferrocyanide | - |
? | |
1.1.9.1 | 2-propanol + ferricyanide | ADH IIB, 52% of the activity with 1-butanol. ADH IIG, 0% of the activity with 1,2-propanediol | Pseudomonas putida | acetone + ferrocyanide | - |
? | |
1.1.9.1 | 3-pentanol + ferricyanide | ADH IIB, 43% of the activity with 1-butanol. ADH IIG, 15% of the activity with 1,2-propanediol | Pseudomonas putida | pentan-3-one + ferrocyanide | - |
? | |
1.1.9.1 | allyl alcohol + ferricyanide | ADH IIB, 160% of the activity with 1-butanol. ADH IIG, 46% of the activity with 1,2-propanediol | Pseudomonas putida | prop-2-enal + ferrocyanide | - |
? | |
1.1.9.1 | butanal + ferrocyanide | ADH IIB, 32% of the activity with 1-butanol. ADH IIG, 13% of the activity with 1,2-propanediol | Pseudomonas putida | 1-butanol + ferricyanide | - |
? | |
1.1.9.1 | ethanal + ferrocyanide | ADH IIB, 78% of the activity with 1-butanol. ADH IIG, 16% of the activity with 1,2-propanediol | Pseudomonas putida | ethanol + ferricyanide | - |
? | |
1.1.9.1 | ethanol + ferricyanide | ADH IIB, 78% of the activity with 1-butanol. ADH IIG, 22% of the activity with 1,2-propanediol | Pseudomonas putida | ethanal + ferrocyanide | - |
? | |
1.1.9.1 | glycerol + ferrocyanide | ADH IIB, 0% of the activity with 1-butanol. ADH IIG, 63% of the activity with 1,2-propanediol | Pseudomonas putida | 2,3-dihydroxypropanal + ferricyanide | - |
? | |
1.1.9.1 | iso-amyl alcohol + ferricyanide | ADH IIB, 100% of the activity with 1-butanol. ADH IIG, 93% of the activity with 1,2-propanediol | Pseudomonas putida | 3-methylbutanol + ferrocyanide | - |
? | |
1.1.9.1 | propanal + ferrocyanide | ADH IIB, 125% of the activity with 1-butanol. ADH IIG, 58% of the activity with 1,2-propanediol | Pseudomonas putida | 1-propanol + ferricyanide | - |
? | |
1.2.5.2 | acetaldehyde + 2 ferricyanide + H2O | - |
Pseudomonas putida | acetate + 2 ferrocyanide + 2 H+ | - |
? | |
1.2.5.2 | acetaldehyde + 2 ferricyanide + H2O | - |
Pseudomonas putida HK5 | acetate + 2 ferrocyanide + 2 H+ | - |
? | |
1.2.5.2 | acetaldehyde + 2,6-dichlorophenol indophenol | in presence of phenazine methosulfate | Pseudomonas putida | acetate + reduced 2,6-dichlorophenol indophenol | - |
? | |
1.2.5.2 | acetaldehyde + 2,6-dichlorophenol indophenol | with phenazine methosulfonate | Pseudomonas putida | acetate + reduced 2,6-dichlorophenol indophenol | - |
? | |
1.2.5.2 | acetaldehyde + 2,6-dichlorophenol indophenol | in presence of phenazine methosulfate | Pseudomonas putida HK5 | acetate + reduced 2,6-dichlorophenol indophenol | - |
? | |
1.2.5.2 | acetaldehyde + 2,6-dichlorophenol indophenol | with phenazine methosulfonate | Pseudomonas putida HK5 | acetate + reduced 2,6-dichlorophenol indophenol | - |
? | |
1.2.5.2 | butanal + 2 ferricyanide + H2O | - |
Pseudomonas putida | butanoate + 2 ferrocyanide + 2 H+ | - |
? | |
1.2.5.2 | butanal + 2 ferricyanide + H2O | - |
Pseudomonas putida HK5 | butanoate + 2 ferrocyanide + 2 H+ | - |
? | |
1.2.5.2 | butyraldehyde + 2,6-dichlorophenol indophenol | in presence of phenazine methosulfate | Pseudomonas putida | butanoate + reduced 2,6-dichlorophenol indophenol | - |
? | |
1.2.5.2 | butyraldehyde + 2,6-dichlorophenol indophenol | with phenazine methosulfonate | Pseudomonas putida | butanoate + reduced 2,6-dichlorophenol indophenol | - |
? | |
1.2.5.2 | butyraldehyde + 2,6-dichlorophenol indophenol | in presence of phenazine methosulfate | Pseudomonas putida HK5 | butanoate + reduced 2,6-dichlorophenol indophenol | - |
? | |
1.2.5.2 | butyraldehyde + 2,6-dichlorophenol indophenol | with phenazine methosulfonate | Pseudomonas putida HK5 | butanoate + reduced 2,6-dichlorophenol indophenol | - |
? | |
1.2.5.2 | additional information | three distinct quinoprotein ADHs (ADHs, ADH IIB and ADH IIG) are synthesized by the bacterium under different growth conditions. ADH I is formed most abundantly in the cells grown on ethanol | Pseudomonas putida | ? | - |
? | |
1.2.5.2 | additional information | three distinct quinoprotein ADHs (ADHs, ADH IIB and ADH IIG) are synthesized by the bacterium under different growth conditions. ADH IIB is induced by n-butanol, 1,3-butandiol, and alcohols of medium chain length | Pseudomonas putida | ? | - |
? | |
1.2.5.2 | additional information | three distinct quinoprotein ADHs (ADHs, ADH IIB and ADH IIG) are synthesized by the bacterium under different growth conditions. Induction of ADH IIG is restricted to 1,2-propanediol or glycerol | Pseudomonas putida | ? | - |
? | |
1.2.5.2 | additional information | ADH I shows no activity with NAD(P), potassium ferricyanide, or molecular oxygen as electron acceptors | Pseudomonas putida | ? | - |
? | |
1.2.5.2 | additional information | isozyme ADH IIG shows no activity with O2 | Pseudomonas putida | ? | - |
? | |
1.2.5.2 | additional information | three distinct quinoprotein ADHs (ADHs, ADH IIB and ADH IIG) are synthesized by the bacterium under different growth conditions. ADH I is formed most abundantly in the cells grown on ethanol | Pseudomonas putida HK5 | ? | - |
? | |
1.2.5.2 | additional information | ADH I shows no activity with NAD(P), potassium ferricyanide, or molecular oxygen as electron acceptors | Pseudomonas putida HK5 | ? | - |
? | |
1.2.5.2 | additional information | three distinct quinoprotein ADHs (ADHs, ADH IIB and ADH IIG) are synthesized by the bacterium under different growth conditions. Induction of ADH IIG is restricted to 1,2-propanediol or glycerol | Pseudomonas putida HK5 | ? | - |
? | |
1.2.5.2 | additional information | isozyme ADH IIG shows no activity with O2 | Pseudomonas putida HK5 | ? | - |
? | |
1.2.5.2 | additional information | three distinct quinoprotein ADHs (ADHs, ADH IIB and ADH IIG) are synthesized by the bacterium under different growth conditions. ADH IIB is induced by n-butanol, 1,3-butandiol, and alcohols of medium chain length | Pseudomonas putida HK5 | ? | - |
? | |
1.2.5.2 | propanal + 2 ferricyanide + H2O | - |
Pseudomonas putida | propanoate + 2 ferrocyanide + 2 H+ | - |
? | |
1.2.5.2 | propanal + 2 ferricyanide + H2O | - |
Pseudomonas putida HK5 | propanoate + 2 ferrocyanide + 2 H+ | - |
? | |
1.2.5.2 | propionaldehyde + 2,6-dichlorophenol indophenol | in presence of phenazine methosulfate | Pseudomonas putida | propanoate + reduced 2,6-dichlorophenol indophenol | - |
? | |
1.2.5.2 | propionaldehyde + 2,6-dichlorophenol indophenol | with phenazine methosulfonate | Pseudomonas putida | propanoate + reduced 2,6-dichlorophenol indophenol | - |
? | |
1.2.5.2 | propionaldehyde + 2,6-dichlorophenol indophenol | in presence of phenazine methosulfate | Pseudomonas putida HK5 | propanoate + reduced 2,6-dichlorophenol indophenol | - |
? | |
1.2.5.2 | propionaldehyde + 2,6-dichlorophenol indophenol | with phenazine methosulfonate | Pseudomonas putida HK5 | propanoate + reduced 2,6-dichlorophenol indophenol | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.9.1 | monomer | ADH IIB, 1 * 69000, SDS-PAGE. ADH IIG, 1 * 72000, SDS-PAGE | Pseudomonas putida |
1.2.5.2 | dimer | 2 * 64000, ADH I, SDS-PAGE | Pseudomonas putida |
1.2.5.2 | monomer | 1 * 69000, ADH IIB | Pseudomonas putida |
1.2.5.2 | monomer | 1 * 72000, ADH IIG | Pseudomonas putida |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.5.5 | ADH I | - |
- |
1.1.5.5 | ADH IIB | - |
- |
1.1.5.5 | ADH IIG | - |
- |
1.1.5.5 | quinohemoprotein dehydrogenase | - |
- |
1.1.9.1 | ADH IIB | - |
Pseudomonas putida |
1.1.9.1 | ADH IIG | - |
Pseudomonas putida |
1.2.5.2 | ADH I | - |
Pseudomonas putida |
1.2.5.2 | ADH IIB | - |
Pseudomonas putida |
1.2.5.2 | ADH IIG | - |
Pseudomonas putida |
1.2.5.2 | quinohemoprotein dehydrogenase | - |
Pseudomonas putida |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.2.5.2 | 8 | - |
about, ADH IIB | Pseudomonas putida |
1.2.5.2 | 8 | - |
about, ADH IIG | Pseudomonas putida |
1.2.5.2 | 9 | - |
about, ADH I | Pseudomonas putida |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.9.1 | heme c | both isoforms ADH IIB and ADH IIG | Pseudomonas putida | |
1.1.9.1 | pyrroloquinoline quinone | both isoforms ADH IIB and ADH IIG | Pseudomonas putida | |
1.2.5.2 | heme c | isozyme ADH IIB is a quinohemoprotein dehydrogenase, determination of heme content, overview | Pseudomonas putida | |
1.2.5.2 | heme c | isozymes ADH IIB and ADH IIG are quinohemoprotein dehydrogenases, while ADH I is a non-heme quinoprotein, determination of heme contents, overview | Pseudomonas putida | |
1.2.5.2 | additional information | ADH I shows no activity with NAD(P), potassium ferricyanide, or molecular oxygen as electron acceptors | Pseudomonas putida | |
1.2.5.2 | pyrroloquinoline quinone | PQQ, a prosthetic group, ADH I is a non-heme quinoprotein containing 2.2 mol of PQQ per mol of enzyme | Pseudomonas putida | |
1.2.5.2 | pyrroloquinoline quinone | PQQ, a prosthetic group, isozyme ADH IIB is a quinohemoprotein dehydrogenase. ADH IIB contains 1.4 mol of PQQ per mol of enzyme on the basis of a molecular weight of 70000 Da | Pseudomonas putida | |
1.2.5.2 | pyrroloquinoline quinone | PQQ, a prosthetic group, isozyme ADH IIG is a quinohemoprotein dehydrogenase. ADH IIG contains 1.2 mol of PQQ per mol of enzyme on the basis of a molecular weight of 70000 Da | Pseudomonas putida |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
1.2.5.2 | Pseudomonas putida | ADH IIG, isoelectric focusing | - |
5 |
1.2.5.2 | Pseudomonas putida | ADH IIB, isoelectric focusing | - |
6.5 |
1.2.5.2 | Pseudomonas putida | ADH I, isoelectric focusing | - |
7.6 |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.1.9.1 | metabolism | ADH IIB is formed in the cells grown on alcohols of medium chain length and also on 1,3-butanediol. Induction of ADH IIG is restricted to 1,2-propanediol or glycerol, of which the former alcohol is more effective | Pseudomonas putida |