BRENDA - Enzyme Database show

Construction of Lactobacillus plantarum strain with enhanced L-lysine yield

Cahyanto, M.N.; Kawasaki, H.; Nagashio, M.; Fujiyama, K.; Seki, T.; J. Appl. Microbiol. 102, 674-679 (2007)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.2.1.11
co-expression with L-lysine-insensitive aspartokinase, dihydrodipicolinate synthase, and dihydrodipicolinate reductase in the parent strain and in an AEC-resistant mutant strain of Lactobacillus plantarum from a shuttle vector, leading to increased L-lysine production, but absolutely requiring overexpression of aspartokinase or dihydrodipicolinate synthase, overview
Lactobacillus plantarum
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.2.1.11
L-aspartate 4-semialdehyde + phosphate + NADP+
Lactobacillus plantarum
-
L-4-aspartyl phosphate + NADPH + H+
-
-
?
1.2.1.11
L-aspartate 4-semialdehyde + phosphate + NADP+
Lactobacillus plantarum IAM 12477
-
L-4-aspartyl phosphate + NADPH + H+
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.2.1.11
Lactobacillus plantarum
-
-
-
1.2.1.11
Lactobacillus plantarum IAM 12477
-
-
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
1.2.1.11
additional information
-
lysine production activity in recombinant strains, overview
Lactobacillus plantarum
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.1.11
L-aspartate 4-semialdehyde + phosphate + NADP+
-
687340
Lactobacillus plantarum
L-4-aspartyl phosphate + NADPH + H+
-
-
-
?
1.2.1.11
L-aspartate 4-semialdehyde + phosphate + NADP+
-
687340
Lactobacillus plantarum IAM 12477
L-4-aspartyl phosphate + NADPH + H+
-
-
-
?
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.2.1.11
NADP+
-
Lactobacillus plantarum
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.2.1.11
co-expression with L-lysine-insensitive aspartokinase, dihydrodipicolinate synthase, and dihydrodipicolinate reductase in the parent strain and in an AEC-resistant mutant strain of Lactobacillus plantarum from a shuttle vector, leading to increased L-lysine production, but absolutely requiring overexpression of aspartokinase or dihydrodipicolinate synthase, overview
Lactobacillus plantarum
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.2.1.11
NADP+
-
Lactobacillus plantarum
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.2.1.11
L-aspartate 4-semialdehyde + phosphate + NADP+
Lactobacillus plantarum
-
L-4-aspartyl phosphate + NADPH + H+
-
-
?
1.2.1.11
L-aspartate 4-semialdehyde + phosphate + NADP+
Lactobacillus plantarum IAM 12477
-
L-4-aspartyl phosphate + NADPH + H+
-
-
?
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
1.2.1.11
additional information
-
lysine production activity in recombinant strains, overview
Lactobacillus plantarum
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.1.11
L-aspartate 4-semialdehyde + phosphate + NADP+
-
687340
Lactobacillus plantarum
L-4-aspartyl phosphate + NADPH + H+
-
-
-
?
1.2.1.11
L-aspartate 4-semialdehyde + phosphate + NADP+
-
687340
Lactobacillus plantarum IAM 12477
L-4-aspartyl phosphate + NADPH + H+
-
-
-
?