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Literature summary extracted from

  • Lai, L.S.; Chang, P.C.; Chang, C.T.
    Isolation and characterization of superoxide dismutase from wheat seedlings (2008), J. Agric. Food Chem., 56, 8121-8129.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.15.1.1 diethyl dicarbonate at 2.5 mM Triticum aestivum
1.15.1.1 HgCl2 1 mM, inhibition of isozyme SODI Triticum aestivum
1.15.1.1 additional information isoyzme SODI is insensitive to H2O2 and KCN Triticum aestivum
1.15.1.1 N-ethyl-5-phenylisoxazolium 3'-sulfonate i.e. Woodward’s reagent, Kat 50 mM Triticum aestivum
1.15.1.1 SDS 1 mM, inhibition of isozyme SODI Triticum aestivum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.15.1.1 Mn2+ isozyme SODI is a Mn-SOD Triticum aestivum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.15.1.1 23000
-
 2 * 23000, isozyme SODI, SDS-PAGE Triticum aestivum
1.15.1.1 46000
-
 isozyme SODI, gel filtration Triticum aestivum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.15.1.1 O2.- + H+ Triticum aestivum
-
 O2 + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.15.1.1 Triticum aestivum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.15.1.1 native isozymes SODI and SODII 154fold and 98fold, respectively, from seedlings by heat treatment at 40°C for 15 min, ammonium sulfate fractionation, anionic exchange chromatography, and gel filtration Triticum aestivum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.15.1.1 seedling
-
 Triticum aestivum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.15.1.1 113.8
-
 purified isozyme SODII Triticum aestivum
1.15.1.1 179
-
 purified isozyme SODI Triticum aestivum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.15.1.1 additional information enzyme activity determination by NBT reduction Triticum aestivum ?
-
 ?
1.15.1.1 O2.- + H+
-
 Triticum aestivum O2 + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.15.1.1 dimer 2 * 23000, isozyme SODI, SDS-PAGE Triticum aestivum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.15.1.1 37
-
 assay at Triticum aestivum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.15.1.1 5 80 temprature profile, overview Triticum aestivum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.15.1.1 5 50 purified isozyme SODI, stable Triticum aestivum
1.15.1.1 50 70 purified isozyme SODI, inactivation, thermal inactivation of wheat seedling MnSOD follows first-order reaction kinetics, and the temperature dependence of rate constants is in agreement with the Arrhenius equation Triticum aestivum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.15.1.1 8
-
 isozyme SODI Triticum aestivum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.15.1.1 7 9 purified isozyme SODI, stable Triticum aestivum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.15.1.1 Triticum aestivum isozyme SODI, isoelectric focusing
-
 4