BRENDA - Enzyme Database

Three polyphenol oxidases from red clover (Trifolium pratense) differ in enzymatic activities and activation properties

Schmitz, G.E.; Sullivan, M.L.; Hatfield, R.D.; J. Agric. Food Chem. 56, 272-280 (2008)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.14.18.1
the cDNAs encoding three red clover PPOs are expressed individually in alfalfa (Medicago sativa L.); the cDNAs encoding three red clover PPOs are expressed individually in alfalfa (Medicago sativa L.); the cDNAs encoding three Trifolium pratensePPOs are expressed individually in alfalfa (Medicago sativa L.)
Trifolium pratense
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.14.18.1
additional information
-
several minor bands (50, 37, and 35 kDa) are observed in PPO2-expressed in alfalfa
Trifolium pratense
1.14.18.1
44000
47000
two protein bands (approximately 44-47 kDa) appear in transgenic alfalfa expressing PPO3
Trifolium pratense
1.14.18.1
45000
-
by 1-2 days of incubation, an additional protein band of approximately 45 kDa becomes visible in extracts of red clover and transgenic alfalfa expressing PPO1 or PPO2
Trifolium pratense
1.14.18.1
65000
-
PPOs expressed in transgenic alfalfa and Trifolium pratense; PPOs expressed in transgenic alfalfa and Trifolium pratense
Trifolium pratense
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.14.18.1
Trifolium pratense
Q84YH9
PPO3, polyphenol oxidase (EC 1.10.3.1) (EC 1.14.18.1)
-
1.14.18.1
Trifolium pratense
Q84YI0
PPO2, polyphenol oxidase (EC 1.10.3.1) (EC 1.14.18.1)
-
1.14.18.1
Trifolium pratense
Q84YI1
PPO1
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.14.18.1
0.0204
-
values shown are normalized to the specific activity (in nmol/s mg) of each PPO for caffeic acid (LtPPO2: 0.34)
Trifolium pratense
1.14.18.1
0.09
-
values shown are normalized to the specific activity of each PPO for caffeic acid (LtPPO3: 1.5 nmol/s mg)
Trifolium pratense
1.14.18.1
0.216
-
values shown are normalized to the specific activity of each PPO for caffeic acid (LtPPO1: 3.6 nmol/s mg)
Trifolium pratense
1.14.18.1
0.348
-
values shown are normalized to the specific activity (in nmol/s mg) of each PPO for caffeic acid (AcPPO2: 5.8)
Trifolium pratense
1.14.18.1
4.08
-
values shown are normalized to the specific activity (in nmol/s mg) of each PPO for caffeic acid (AcPPO3, 68 nmol/s mg)
Trifolium pratense
1.14.18.1
5.94
-
values shown are normalized to the specific activity (in nmol/s mg) of each PPO for caffeic acid (AcPPO1: 99 nmol/s mg)
Trifolium pratense
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.18.1
(+)-catechin + 1/2 O2
-
687250
Trifolium pratense
?
-
-
-
?
1.14.18.1
(-)-epicatechin + 1/2 O2
-
687250
Trifolium pratense
?
-
-
-
?
1.14.18.1
caffeic acid + 1/2 O2
-
687250
Trifolium pratense
caffeoyl quinone + H2O
-
-
-
?
1.14.18.1
catechol + 1/2 O2
-
687250
Trifolium pratense
1,2-benzoquinone + H2O
-
-
-
?
1.14.18.1
chlorogenic acid + 1/2 O2
-
687250
Trifolium pratense
chlorogenoquinone + H2O
-
-
-
?
1.14.18.1
dopamine + 1/2 O2
-
687250
Trifolium pratense
dopaminequinone + H2O
-
-
-
?
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.14.18.1
additional information
-
PPO1 appears to be more stable at high temperatures than PPO2 or PPO3; PPO1 appears to be more stable at high temperatures than PPO2 or PPO3; PPO1 appears to be more stable at high temperatures than PPO2 or PPO3
Trifolium pratense
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.14.18.1
5.1
-
for PPO3, activation of the PPOs shifts the pH optima to 7
Trifolium pratense
1.14.18.1
5.5
-
for PPO1, activation of the PPOs shifts the pH optima to 7
Trifolium pratense
1.14.18.1
6.9
-
for PPO2, activation of the PPOs shifts the pH optima to 7
Trifolium pratense
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.18.1
the cDNAs encoding three red clover PPOs are expressed individually in alfalfa (Medicago sativa L.)
Trifolium pratense
1.14.18.1
the cDNAs encoding three Trifolium pratensePPOs are expressed individually in alfalfa (Medicago sativa L.)
Trifolium pratense
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.14.18.1
additional information
-
several minor bands (50, 37, and 35 kDa) are observed in PPO2-expressed in alfalfa
Trifolium pratense
1.14.18.1
44000
47000
two protein bands (approximately 44-47 kDa) appear in transgenic alfalfa expressing PPO3
Trifolium pratense
1.14.18.1
45000
-
by 1-2 days of incubation, an additional protein band of approximately 45 kDa becomes visible in extracts of red clover and transgenic alfalfa expressing PPO1 or PPO2
Trifolium pratense
1.14.18.1
65000
-
PPOs expressed in transgenic alfalfa and Trifolium pratense
Trifolium pratense
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.14.18.1
0.0204
-
values shown are normalized to the specific activity (in nmol/s mg) of each PPO for caffeic acid (LtPPO2: 0.34)
Trifolium pratense
1.14.18.1
0.09
-
values shown are normalized to the specific activity of each PPO for caffeic acid (LtPPO3: 1.5 nmol/s mg)
Trifolium pratense
1.14.18.1
0.216
-
values shown are normalized to the specific activity of each PPO for caffeic acid (LtPPO1: 3.6 nmol/s mg)
Trifolium pratense
1.14.18.1
0.348
-
values shown are normalized to the specific activity (in nmol/s mg) of each PPO for caffeic acid (AcPPO2: 5.8)
Trifolium pratense
1.14.18.1
4.08
-
values shown are normalized to the specific activity (in nmol/s mg) of each PPO for caffeic acid (AcPPO3, 68 nmol/s mg)
Trifolium pratense
1.14.18.1
5.94
-
values shown are normalized to the specific activity (in nmol/s mg) of each PPO for caffeic acid (AcPPO1: 99 nmol/s mg)
Trifolium pratense
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.18.1
(+)-catechin + 1/2 O2
-
687250
Trifolium pratense
?
-
-
-
?
1.14.18.1
(-)-epicatechin + 1/2 O2
-
687250
Trifolium pratense
?
-
-
-
?
1.14.18.1
caffeic acid + 1/2 O2
-
687250
Trifolium pratense
caffeoyl quinone + H2O
-
-
-
?
1.14.18.1
catechol + 1/2 O2
-
687250
Trifolium pratense
1,2-benzoquinone + H2O
-
-
-
?
1.14.18.1
chlorogenic acid + 1/2 O2
-
687250
Trifolium pratense
chlorogenoquinone + H2O
-
-
-
?
1.14.18.1
dopamine + 1/2 O2
-
687250
Trifolium pratense
dopaminequinone + H2O
-
-
-
?
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.14.18.1
additional information
-
PPO1 appears to be more stable at high temperatures than PPO2 or PPO3
Trifolium pratense
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.14.18.1
5.1
-
for PPO3, activation of the PPOs shifts the pH optima to 7
Trifolium pratense
1.14.18.1
5.5
-
for PPO1, activation of the PPOs shifts the pH optima to 7
Trifolium pratense
1.14.18.1
6.9
-
for PPO2, activation of the PPOs shifts the pH optima to 7
Trifolium pratense