EC Number | Application | Comment | Organism |
---|---|---|---|
1.1.1.27 | synthesis | the enzyme might be useful in the production of phenyllactate | Lactobacillus sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.27 | 0.32 | - |
pyruvate | pH 6.5, 30°C, purified enzyme | Lactobacillus sp. | |
1.1.1.27 | 1.76 | - |
phenylpyruvate | pH 6.5, 30°C, purified enzyme | Lactobacillus sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.27 | 39000 | - |
2 * 39000, SDS-PAGE | Lactobacillus sp. |
1.1.1.27 | 78000 | - |
gel filtration | Lactobacillus sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.27 | (S)-lactate + NAD+ | Lactobacillus sp. | - |
pyruvate + NADH + H+ | - |
r | |
1.1.1.27 | (S)-lactate + NAD+ | Lactobacillus sp. SK007 | - |
pyruvate + NADH + H+ | - |
r | |
1.1.1.27 | phenylpyruvate + NADH | Lactobacillus sp. | - |
phenyllactate + NAD+ | - |
r | |
1.1.1.27 | phenylpyruvate + NADH | Lactobacillus sp. SK007 | - |
phenyllactate + NAD+ | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.27 | Lactobacillus sp. | - |
- |
- |
1.1.1.27 | Lactobacillus sp. SK007 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.27 | native enzyme 34.3fold to about 98% purity by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration | Lactobacillus sp. |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.27 | 203.3 | - |
purified native enzyme, substrate pyruvate | Lactobacillus sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.27 | (S)-lactate + NAD+ | - |
Lactobacillus sp. | pyruvate + NADH + H+ | - |
r | |
1.1.1.27 | (S)-lactate + NAD+ | - |
Lactobacillus sp. SK007 | pyruvate + NADH + H+ | - |
r | |
1.1.1.27 | phenylpyruvate + NADH | - |
Lactobacillus sp. | phenyllactate + NAD+ | - |
r | |
1.1.1.27 | phenylpyruvate + NADH | - |
Lactobacillus sp. SK007 | phenyllactate + NAD+ | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.27 | dimer | 2 * 39000, SDS-PAGE | Lactobacillus sp. |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.27 | lactate dehydrogenase | - |
Lactobacillus sp. |
1.1.1.27 | LDH | - |
Lactobacillus sp. |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.27 | 40 | - |
with substrate phenylpyruvate | Lactobacillus sp. |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.27 | 20 | 70 | 90% of maximal activity at 50°C and 50% at 60°C | Lactobacillus sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.27 | 6 | - |
with substrate phenylpyruvate | Lactobacillus sp. |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.27 | 3 | 9 | activity range, the enzyme is inactive below pH 3.0 and above pH 9.0 | Lactobacillus sp. |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.27 | 4 | 8 | rather stable from pH 4.0 to pH 8.0, but rapid decline in activity below pH 4.0 and above pH 8.0 | Lactobacillus sp. |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.27 | NAD+ | - |
Lactobacillus sp. | |
1.1.1.27 | NADH | - |
Lactobacillus sp. |