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Literature summary extracted from

  • Li, X.; Jiang, B.; Pan, B.; Mu, W.; Zhang, T.
    Purification and partial characterization of Lactobacillus species SK007 lactate dehydrogenase (LDH) catalyzing phenylpyruvic acid (PPA) conversion into phenyllactic acid (PLA) (2008), J. Agric. Food Chem., 56, 2392-2399.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.1.1.27 synthesis the enzyme might be useful in the production of phenyllactate Lactobacillus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.27 0.32
-
pyruvate pH 6.5, 30°C, purified enzyme Lactobacillus sp.
1.1.1.27 1.76
-
phenylpyruvate pH 6.5, 30°C, purified enzyme Lactobacillus sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.27 39000
-
2 * 39000, SDS-PAGE Lactobacillus sp.
1.1.1.27 78000
-
gel filtration Lactobacillus sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.27 (S)-lactate + NAD+ Lactobacillus sp.
-
pyruvate + NADH + H+
-
r
1.1.1.27 (S)-lactate + NAD+ Lactobacillus sp. SK007
-
pyruvate + NADH + H+
-
r
1.1.1.27 phenylpyruvate + NADH Lactobacillus sp.
-
phenyllactate + NAD+
-
r
1.1.1.27 phenylpyruvate + NADH Lactobacillus sp. SK007
-
phenyllactate + NAD+
-
r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.27 Lactobacillus sp.
-
-
-
1.1.1.27 Lactobacillus sp. SK007
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.27 native enzyme 34.3fold to about 98% purity by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration Lactobacillus sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.27 203.3
-
purified native enzyme, substrate pyruvate Lactobacillus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.27 (S)-lactate + NAD+
-
Lactobacillus sp. pyruvate + NADH + H+
-
r
1.1.1.27 (S)-lactate + NAD+
-
Lactobacillus sp. SK007 pyruvate + NADH + H+
-
r
1.1.1.27 phenylpyruvate + NADH
-
Lactobacillus sp. phenyllactate + NAD+
-
r
1.1.1.27 phenylpyruvate + NADH
-
Lactobacillus sp. SK007 phenyllactate + NAD+
-
r

Subunits

EC Number Subunits Comment Organism
1.1.1.27 dimer 2 * 39000, SDS-PAGE Lactobacillus sp.

Synonyms

EC Number Synonyms Comment Organism
1.1.1.27 lactate dehydrogenase
-
Lactobacillus sp.
1.1.1.27 LDH
-
Lactobacillus sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.27 40
-
with substrate phenylpyruvate Lactobacillus sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.27 20 70 90% of maximal activity at 50°C and 50% at 60°C Lactobacillus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.27 6
-
with substrate phenylpyruvate Lactobacillus sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.27 3 9 activity range, the enzyme is inactive below pH 3.0 and above pH 9.0 Lactobacillus sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.27 4 8 rather stable from pH 4.0 to pH 8.0, but rapid decline in activity below pH 4.0 and above pH 8.0 Lactobacillus sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.27 NAD+
-
Lactobacillus sp.
1.1.1.27 NADH
-
Lactobacillus sp.