EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.4.1.5 | 0.012 | - |
L-glutamine | in 100 mM glycine-KCl-KOH buffer (pH 10.4) | Proteus sp. | |
1.4.1.5 | 0.025 | - |
L-isoleucine | in 100 mM glycine-KCl-KOH buffer (pH 10.4) | Citrobacter sp. | |
1.4.1.5 | 0.034 | - |
L-methionine | in 100 mM glycine-KCl-KOH buffer (pH 10.4) | Proteus sp. | |
1.4.1.5 | 0.044 | - |
L-valine | in 100 mM glycine-KCl-KOH buffer (pH 10.4) | Pseudomonas sp. |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.4.1.5 | Citrobacter sp. | - |
- |
- |
1.4.1.5 | Proteus sp. | - |
- |
- |
1.4.1.5 | Pseudomonas sp. | - |
- |
- |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.4.1.5 | 0.47 | - |
using L-valine, L-cysteine, L-serine, or L-glutamine as substrate, in 100 mM glycine-KCl-KOH buffer (pH 10.4) | Pseudomonas sp. |
1.4.1.5 | 0.7 | - |
using L-glutamine as substrate, in 100 mM glycine-KCl-KOH buffer (pH 10.4) | Citrobacter sp. |
1.4.1.5 | 0.73 | - |
using L-cysteine, L-serine, or L-glutamine as substrate, in 100 mM glycine-KCl-KOH buffer (pH 10.4) | Proteus sp. |
1.4.1.5 | 1.2 | - |
using L-methionine as substrate, in 100 mM glycine-KCl-KOH buffer (pH 10.4) | Proteus sp. |
1.4.1.5 | 1.59 | - |
using L-isoleucine as substrate, in 100 mM glycine-KCl-KOH buffer (pH 10.4) | Citrobacter sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.4.1.5 | L-cysteine + H2O + NAD+ | - |
Pseudomonas sp. | 3-mercapto-2-oxopropanoate + NH3 + NADH | - |
? | |
1.4.1.5 | L-cysteine + H2O + NAD+ | - |
Citrobacter sp. | 3-mercapto-2-oxopropanoate + NH3 + NADH | - |
? | |
1.4.1.5 | L-cysteine + H2O + NAD+ | - |
Proteus sp. | 3-mercapto-2-oxopropanoate + NH3 + NADH | - |
? | |
1.4.1.5 | L-glutamine + H2O + NAD+ | - |
Pseudomonas sp. | 2-oxoglutaramate + NH3 + NADH | - |
? | |
1.4.1.5 | L-glutamine + H2O + NAD+ | - |
Citrobacter sp. | 2-oxoglutaramate + NH3 + NADH | - |
? | |
1.4.1.5 | L-glutamine + H2O + NAD+ | - |
Proteus sp. | 2-oxoglutaramate + NH3 + NADH | - |
? | |
1.4.1.5 | L-isoleucine + H2O + NAD+ | best substrate | Citrobacter sp. | 3-methyl-2-oxopentanoate + NH3 + NADH | - |
? | |
1.4.1.5 | L-methionine + H2O + NAD+ | - |
Proteus sp. | (4-methylsulfanyl)-2-oxobutanoate + NH3 + NADH | - |
? | |
1.4.1.5 | L-serine + H2O + NAD+ | - |
Pseudomonas sp. | 3-hydroxy-2-oxopropanoate + NH3 + NADH | - |
? | |
1.4.1.5 | L-serine + H2O + NAD+ | - |
Citrobacter sp. | 3-hydroxy-2-oxopropanoate + NH3 + NADH | - |
? | |
1.4.1.5 | L-serine + H2O + NAD+ | - |
Proteus sp. | 3-hydroxy-2-oxopropanoate + NH3 + NADH | - |
? | |
1.4.1.5 | L-valine + H2O + NAD+ | highest activity | Pseudomonas sp. | 3-methyl-2-oxobutanoate + NH3 + NADH | - |
? | |
1.4.1.5 | additional information | no amino acid dehydrogenase activity for L-asparagine | Proteus sp. | ? | - |
? | |
1.4.1.5 | additional information | no amino acid dehydrogenase activity for L-isoleucine amino acid analogs including L-leucine, L-valine, L-alanine or L-methionine | Citrobacter sp. | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.4.1.5 | NAD+-dependent amino acid dehydrogenase | - |
Pseudomonas sp. |
1.4.1.5 | NAD+-dependent amino acid dehydrogenase | - |
Citrobacter sp. |
1.4.1.5 | NAD+-dependent amino acid dehydrogenase | - |
Proteus sp. |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.4.1.5 | 1.15 | - |
L-glutamine | in 100 mM glycine-KCl-KOH buffer (pH 10.4) | Proteus sp. | |
1.4.1.5 | 2.11 | - |
L-methionine | in 100 mM glycine-KCl-KOH buffer (pH 10.4) | Proteus sp. | |
1.4.1.5 | 2.84 | - |
L-isoleucine | in 100 mM glycine-KCl-KOH buffer (pH 10.4) | Citrobacter sp. | |
1.4.1.5 | 4.95 | - |
L-valine | in 100 mM glycine-KCl-KOH buffer (pH 10.4) | Pseudomonas sp. |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.4.1.5 | NAD+ | dependent | Pseudomonas sp. | |
1.4.1.5 | NAD+ | dependent | Citrobacter sp. | |
1.4.1.5 | NAD+ | dependent | Proteus sp. |