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Literature summary extracted from

  • Gouzi, H.; Benmansour, A.
    Partial purification and characterization of polyphenol oxidase extracted from Agaricus bisporus (J.E.Lange) Imbach (2007), Int. J. Chem. React. Eng., 5, A76.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.18.1 benzoic acid effects of inhibitors on mushroom PPO are determined by using pyrogallol as substrate Agaricus bisporus
1.14.18.1 L-cysteine effects of inhibitors on mushroom PPO are determined by using pyrogallol as substrate Agaricus bisporus
1.14.18.1 Sodium azide effects of inhibitors on mushroom PPO are determined by using pyrogallol as substrate Agaricus bisporus
1.14.18.1 Sodium fluoride effects of inhibitors on mushroom PPO are determined by using pyrogallol as substrate Agaricus bisporus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.18.1 0.4
-
 pyrocatechol Lineweaver-Burk plot of pyrocatechol oxidation by the mushroom PPO Agaricus bisporus
1.14.18.1 1.4
-
 pyrogallol Lineweaver-Burk plot of pyrogallol oxidation by the mushroom PPO Agaricus bisporus

Organism

EC Number Organism UniProt Comment Textmining
1.14.18.1 Agaricus bisporus
-
 Agaricus bisporus (J.E.Lange) Imbach
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.18.1 a 2fold purification in monophenolase and diphenolase activities is achieved by ammonium sulfate fractionation Agaricus bisporus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.18.1 additional information
-
 the monophenolase activity is 3.35 EU/ml, and the diphenolase activity is 189.3 EU/ml. The specific acitivity after purification is 0.71 EU/mg for cresolase and 40.1 EU/mg for catecholase Agaricus bisporus

Storage Stability

EC Number Storage Stability Organism
1.14.18.1 PPO is relatively stable at -15°C for 44 days. Agaricus bisporus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.18.1 L-tyrosine + O2 + AH2
-
 Agaricus bisporus L-dopa + H2O + A
-
 ?
1.14.18.1 additional information the enzyme shows low activity using mono- and triphenols as substrates but much greater activity with the diphenolic substrate Agaricus bisporus ?
-
 ?
1.14.18.1 phenol + O2 + AH2
-
 Agaricus bisporus catechol + H2O + A
-
 ?
1.14.18.1 phloroglucinol + 1/2 O2
-
 Agaricus bisporus ?
-
 ?
1.14.18.1 pyrocatechol + 1/2 O2
-
 Agaricus bisporus 1,2-benzoquinone + H2O
-
 ?
1.14.18.1 pyrogallol + 1/2 O2
-
 Agaricus bisporus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.18.1 monophenol, o-diphenol:oxygen oxidoreductase
-
 Agaricus bisporus
1.14.18.1 polyphenol oxidase
-
 Agaricus bisporus
1.14.18.1 PPO
-
 Agaricus bisporus
1.14.18.1 tyrosinase a real tyrosinase because it shows both diphenolase and monophenolase activities Agaricus bisporus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.14.18.1 25 70 determination of the temperature stability of mushroom PPO Agaricus bisporus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.14.18.1 35
-
 the enzyme is not very heat stable, and its activity decreases when incubated at the temperatures higher than 35°C Agaricus bisporus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.18.1 5.3
-
 PPO activity shows two pH optima, at 5.3 and 7.0 at 25°C when pyrogallol is used as the substrate Agaricus bisporus
1.14.18.1 7
-
 PPO activity shows two pH optima, at 5.3 and 7.0 at 25°C when pyrogallol is used as the substrate Agaricus bisporus