Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Fang, J.; Deng, D.; Nakamura, H.; Akuta, T.; Qin, H.; Iyer, A.K.; Greish, K.; Maeda, H.
    Oxystress inducing antitumor therapeutics via tumor-targeted delivery of PEG-conjugated D-amino acid oxidase (2008), Int. J. Cancer, 122, 1135-1144.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.4.3.3 medicine polyethylene glycol-conjugated D-amino acid oxidase exhibits potent antitumor activity by generating toxic reactive oxygen species, namely oxidation therapy, subsequently shows remarkable antitumor effect on murine sarcoma 180 solid tumor Sus scrofa

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.3.3 expressed in Escherichia coli strain BL21 (DE3) Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.3.3 39000
-
 2 * 39000, native D-amino acid oxidase, SDS-PAGE Sus scrofa
1.4.3.3 65000
-
 2 * 65000, recombinant polyethylene glycol-conjugated D-amino acid oxidase, SDS-PAGE Sus scrofa
1.4.3.3 113000
-
 recombinant polyethylene glycol-conjugated D-amino acid oxidase, gel filtration Sus scrofa

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
1.4.3.3 Triton X-114 less than 10% decrease of enzyme activity after Triton X-114 treatment, sonication, lyophilization or repeated freezing-thawing Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.3 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.3.3 HiTrap DEAE column chromatography Sus scrofa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.3.3 4.6
-
 recombinant polyethylene glycol-conjugated D-amino acid oxidase, at 25°C in 0.1 M Tris-HCl buffer (pH 8.2) Sus scrofa
1.4.3.3 5.3
-
 native enzyme, at 25°C in 0.1 M Tris-HCl buffer (pH 8.2) Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.3 cephalosporin C + H2O + O2
-
 Sus scrofa 7-(5-oxoadipoamido)cephalosporanic acid + NH3 + H2O2
-
 ?
1.4.3.3 D-alanine + H2O + O2
-
 Sus scrofa pyruvate + NH3 + H2O2
-
 ?
1.4.3.3 D-methionine + H2O + O2
-
 Sus scrofa 4-methylthio-2-oxobutanoic acid + NH3 + H2O2
-
 ?
1.4.3.3 D-proline + H2O + O2
-
 Sus scrofa 2-oxopentanoic acid + NH3 + H2O2
-
 ?
1.4.3.3 glycine + 2 H2O + O2
-
 Sus scrofa 2 formic acid + NH3 + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.3.3 homodimer 2 * 39000, native D-amino acid oxidase, SDS-PAGE Sus scrofa
1.4.3.3 homodimer 2 * 65000, recombinant polyethylene glycol-conjugated D-amino acid oxidase, SDS-PAGE Sus scrofa

Synonyms

EC Number Synonyms Comment Organism
1.4.3.3 D-amino acid oxidase
-
 Sus scrofa
1.4.3.3 PEG-DAO
-
 Sus scrofa

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.3.3 FAD
-
 Sus scrofa