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Literature summary extracted from

  • Zhang, W.; Jones, V.C.; Scherman, M.S.; Mahapatra, S.; Crick, D.; Bhamidi, S.; Xin, Y.; McNeil, M.R.; Ma, Y.
    Expression, essentiality, and a microtiter plate assay for mycobacterial GlmU, the bifunctional glucosamine-1-phosphate acetyltransferase and N-acetylglucosamine-1-phosphate uridyltransferase (2008), Int. J. Biochem. Cell Biol., 40, 2560-2571.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.3.1.4 molecular biology a microtiter plate based assay to detect the GlmU activity is developed: the assay relies on the enzymes MurA and MurB to convert UDP-GlcNAc into UDP-MurNAc with the concomitant reduction of NADPH. When all enzymes and substrates are present NADPH is oxidized with a concomitant decrease in OD340 Mycobacterium tuberculosis
2.3.1.157 medicine GlmU is a potential antituberculosis drug target, a microtiter plate assay for GlmU activity as read out is developed Mycobacterium tuberculosis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.4 expressed in Escherichia coli Mycobacterium tuberculosis
2.3.1.157 expression in Escherichia coli, expression in Mycobacterium smegmatis ZW-2, a GlmU knoukout strain, activity of GlmU is required for growth of Mycobacterium smegmatis, a gradually reduced enzyme activity in a temperature shift experiment shows that the Mycobacterium smegmatis cells became non-viable and their morphology changes from a normal rod shape to stubby-rounded morphology Mycobacterium tuberculosis

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.4 additional information generation of a GlmU knockout strain discloses that GlmU is required for growth of Mycobacterium smegmatis as the bacteria does not grow in the absence of active GlmU enzyme Mycolicibacterium smegmatis
2.3.1.4 additional information in a temperature shift experiment (from 30°C to 42°C) the amount of functional GlmU enzyme is gradually reduced the Mycobacterium smegmatis cells become non-viable and their morphology change from a normal rod shape to stubby-rounded morphology and in some cases they lyse Mycolicibacterium smegmatis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.1.4 Mg2+
-
 Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.4 54100
-
 SDS-PAGE Mycobacterium tuberculosis
2.3.1.157 54100
-
 SDS-PAGE, Western blot Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.4 Mycobacterium tuberculosis
-
-
-
2.3.1.4 Mycolicibacterium smegmatis
-
-
-
2.3.1.157 Mycobacterium tuberculosis P9WMN3
-
-
2.3.1.157 Mycobacterium tuberculosis H37Rv P9WMN3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.4 using a HIS-Select HF affinity gel column Mycobacterium tuberculosis
2.3.1.157 the recombinant protein by Ni2+ affinity chromatography Mycobacterium tuberculosis

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.157 acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + N-acetyl-alpha-D-glucosamine 1-phosphate also N-acetyl-alpha-D-glucosamine 1-phosphate + UTP = UDP-N-acetyl-D-glucosamine + diphosphate Mycobacterium tuberculosis
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.4 acetyl-CoA + D-glucosamine 6-phosphate
-
 Mycolicibacterium smegmatis CoA + N-acetyl-D-glucosamine 6-phosphate
-
 ?
2.3.1.4 acetyl-CoA + D-glucosamine 6-phosphate
-
 Mycobacterium tuberculosis CoA + N-acetyl-D-glucosamine 6-phosphate
-
 ?
2.3.1.157 acetyl-CoA + alpha-D-glucosamine 1-phosphate
-
 Mycobacterium tuberculosis CoA + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 ?
2.3.1.157 acetyl-CoA + alpha-D-glucosamine 1-phosphate
-
 Mycobacterium tuberculosis H37Rv CoA + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 ?
2.3.1.157 UDP-N-acetyl-d-glucosamine + diphosphate
-
 Mycobacterium tuberculosis N-acetyl-alpha-D-glucosamine 1-phosphate + UTP
-
 r
2.3.1.157 UDP-N-acetyl-d-glucosamine + diphosphate
-
 Mycobacterium tuberculosis H37Rv N-acetyl-alpha-D-glucosamine 1-phosphate + UTP
-
 r

Synonyms

EC Number Synonyms Comment Organism
2.3.1.4 GlmU GlmU protein exhibits both glucosamine-1-phosphate acetyltransferase and N-acetylglucosamine-1-phosphate uridylyltransferase activities Mycolicibacterium smegmatis
2.3.1.4 GlmU GlmU protein exhibits both glucosamine-1-phosphate acetyltransferase and N-acetylglucosamine-1-phosphate uridylyltransferase activities Mycobacterium tuberculosis
2.3.1.4 glucosamine-6-phosphate N-acetyltransferase
-
 Mycolicibacterium smegmatis
2.3.1.4 glucosamine-6-phosphate N-acetyltransferase
-
 Mycobacterium tuberculosis
2.3.1.157 GlmU
-
 Mycobacterium tuberculosis
2.3.1.157 glucosamine-1-phosphate acetyltransferase bifuncional enzyme, also N-acetylglucosamine-1-phosphate uridyltransferase activity Mycobacterium tuberculosis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.4 additional information
-
 assay carried out at room temperature Mycobacterium tuberculosis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.4 8
-
 assay at Mycobacterium tuberculosis