BRENDA - Enzyme Database

Purification and biochemical characterization of a recombinant Anopheles gambiae tryptophan 2,3-dioxygenase expressed in Escherichia coli

Paglino, A.; Lombardo, F.; Arca, B.; Rizzi, M.; Rossi, F.; Insect Biochem. Mol. Biol. 38, 871-876 (2008)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.13.11.52
expression of Ag-HisTDO in Escherichia coli
Anopheles gambiae
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.13.11.52
3-hydroxykynurenine
0.1 mM, 89.8% inhibition
Anopheles gambiae
1.13.11.52
5-hydroxyindole acetic acid
1 mM, 61.5% inhibition
Anopheles gambiae
1.13.11.52
5-hydroxytryptamine
1 mM, 82.3% inhibition
Anopheles gambiae
1.13.11.52
5-hydroxytryptophan
1 mM, 78.6% inhibition
Anopheles gambiae
1.13.11.52
Kynurenic acid
0.1 mM, 57.2% inhibition. 1 mM, 99.4% inhibition
Anopheles gambiae
1.13.11.52
L-kynurenine
0.1 mM, 17.7% inhibition. 1 mM, 93.6% inhibition
Anopheles gambiae
1.13.11.52
NADH
0.1 mM, 51.3% inhibition
Anopheles gambiae
1.13.11.52
xanthurenic acid
0.1 mM, 50.6% inhibition. 1 mM, 96.2% inhibition
Anopheles gambiae
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.13.11.52
0.6
-
L-tryptophan
-
Anopheles gambiae
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.13.11.52
47900
-
x * 47900, SDS-PAGE
Anopheles gambiae
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.13.11.52
L-tryptophan + O2
Anopheles gambiae
tryptophan 2,3-dioxygenase is the only enzyme able to initiate L-tryptophan degradation through the kynurenine pathway
N-formyl-L-kynurenine
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.13.11.52
Anopheles gambiae
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.13.11.52
-
Anopheles gambiae
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.13.11.52
1.43
-
-
Anopheles gambiae
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.13.11.52
L-tryptophan + O2
-
687095
Anopheles gambiae
N-formyl-L-kynurenine
-
-
-
?
1.13.11.52
L-tryptophan + O2
tryptophan 2,3-dioxygenase is the only enzyme able to initiate L-tryptophan degradation through the kynurenine pathway
687095
Anopheles gambiae
N-formyl-L-kynurenine
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.13.11.52
?
x * 47900, SDS-PAGE
Anopheles gambiae
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.13.11.52
2.03
-
L-tryptophan
-
Anopheles gambiae
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.13.11.52
8
-
-
Anopheles gambiae
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.13.11.52
6.5
9
pH 6.5: about 50% of maximal activity, pH 9.0: about 70% of maximal activity
Anopheles gambiae
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.13.11.52
heme
Ag-HisTDO is purified in its heme-ferric form. The prosthetic group can be fully converted into the heme-ferrous form by treatment with sodium dithionite
Anopheles gambiae
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.13.11.52
expression of Ag-HisTDO in Escherichia coli
Anopheles gambiae
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.13.11.52
heme
Ag-HisTDO is purified in its heme-ferric form. The prosthetic group can be fully converted into the heme-ferrous form by treatment with sodium dithionite
Anopheles gambiae
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.13.11.52
3-hydroxykynurenine
0.1 mM, 89.8% inhibition
Anopheles gambiae
1.13.11.52
5-hydroxyindole acetic acid
1 mM, 61.5% inhibition
Anopheles gambiae
1.13.11.52
5-hydroxytryptamine
1 mM, 82.3% inhibition
Anopheles gambiae
1.13.11.52
5-hydroxytryptophan
1 mM, 78.6% inhibition
Anopheles gambiae
1.13.11.52
Kynurenic acid
0.1 mM, 57.2% inhibition. 1 mM, 99.4% inhibition
Anopheles gambiae
1.13.11.52
L-kynurenine
0.1 mM, 17.7% inhibition. 1 mM, 93.6% inhibition
Anopheles gambiae
1.13.11.52
NADH
0.1 mM, 51.3% inhibition
Anopheles gambiae
1.13.11.52
xanthurenic acid
0.1 mM, 50.6% inhibition. 1 mM, 96.2% inhibition
Anopheles gambiae
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.13.11.52
0.6
-
L-tryptophan
-
Anopheles gambiae
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.13.11.52
47900
-
x * 47900, SDS-PAGE
Anopheles gambiae
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.13.11.52
L-tryptophan + O2
Anopheles gambiae
tryptophan 2,3-dioxygenase is the only enzyme able to initiate L-tryptophan degradation through the kynurenine pathway
N-formyl-L-kynurenine
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.13.11.52
-
Anopheles gambiae
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.13.11.52
1.43
-
-
Anopheles gambiae
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.13.11.52
L-tryptophan + O2
-
687095
Anopheles gambiae
N-formyl-L-kynurenine
-
-
-
?
1.13.11.52
L-tryptophan + O2
tryptophan 2,3-dioxygenase is the only enzyme able to initiate L-tryptophan degradation through the kynurenine pathway
687095
Anopheles gambiae
N-formyl-L-kynurenine
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.13.11.52
?
x * 47900, SDS-PAGE
Anopheles gambiae
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.13.11.52
2.03
-
L-tryptophan
-
Anopheles gambiae
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.13.11.52
8
-
-
Anopheles gambiae
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.13.11.52
6.5
9
pH 6.5: about 50% of maximal activity, pH 9.0: about 70% of maximal activity
Anopheles gambiae