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Literature summary extracted from

  • Loumaye, E.; Andersen, A.C.; Clippe, A.; Degand, H.; Dubuisson, M.; Zal, F.; Morsomme, P.; Rees, J.F.; Knoops, B.
    Cloning and characterization of Arenicola marina peroxiredoxin 6, an annelid two-cysteine peroxiredoxin highly homologous to mammalian one-cysteine peroxiredoxins (2008), Free Radic. Biol. Med., 45, 482-493.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.11.1.24 expressed in Escherichia coli Arenicola marina

Protein Variants

EC Number Protein Variants Comment Organism
1.11.1.24 C183S strongly reduced activity Arenicola marina
1.11.1.24 C45S inactive Arenicola marina

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.1.24 22000
-
 1 * 22000, SDS-PAGE, under atmospheric oxidations the protein appears as a small oxidized monomer and as a major oxidized dimer Arenicola marina
1.11.1.24 25000
-
 1 * 25000, SDS-PAGE, after reduction with 10 mM dithiothreitol the protein appears as a reduced monomer Arenicola marina
1.11.1.24 50000
-
 2 * 50000, SDS-PAGE, under atmospheric oxidations the protein appears as a small oxidized monomer and as a major oxidized dimer Arenicola marina

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.24 Arenicola marina Q1AN22
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.24 Ni2+ column chromatography Arenicola marina

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.11.1.24 body wall
-
 Arenicola marina
-
1.11.1.24 gill
-
 Arenicola marina
-
1.11.1.24 additional information no activity is detected in muscles, coelomic fluid, and other internal structures Arenicola marina
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.24 cumene hydroperoxide + reduced thioredoxin
-
 Arenicola marina 2-phenylpropan-2-ol + oxidized thioredoxin + H2O
-
 ?
1.11.1.24 tert-butyl hydroperoxide + reduced dithiothreitol
-
 Arenicola marina tert-butanol + oxidized dithiothreitol
-
 ?

Subunits

EC Number Subunits Comment Organism
1.11.1.24 homodimer 2 * 50000, SDS-PAGE, under atmospheric oxidations the protein appears as a small oxidized monomer and as a major oxidized dimer Arenicola marina
1.11.1.24 monomer 1 * 22000, SDS-PAGE, under atmospheric oxidations the protein appears as a small oxidized monomer and as a major oxidized dimer Arenicola marina
1.11.1.24 monomer 1 * 25000, SDS-PAGE, after reduction with 10 mM dithiothreitol the protein appears as a reduced monomer Arenicola marina

Synonyms

EC Number Synonyms Comment Organism
1.11.1.24 peroxiredoxin 6
-
 Arenicola marina
1.11.1.24 Prdx6 classified as a 2-Cys PRDX Arenicola marina

Cofactor

EC Number Cofactor Comment Organism Structure
1.11.1.24 thioredoxin
-
 Arenicola marina