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Literature summary extracted from

  • Gawlik-Dziki, U.; Szymanowska, U.; Baraniak, B.
    Characterization of polyphenol oxidase from broccoli (Brassica oleracea var. botrytis italica) florets (2007), Food Chem., 105, 1047-1053.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.18.1 ascorbic acid noncompetitive inhibition Brassica oleracea
1.14.18.1 Citric acid noncompetitive inhibition Brassica oleracea
1.14.18.1 EDTA sodium salt of ethylenediaminetetraacetic acid, uncompetitive inhibition Brassica oleracea
1.14.18.1 sodium sulphate most effective inhibitor, noncompetitive inhibition Brassica oleracea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.18.1 12.34
-
 catechol polyphenol oxidase shows the best activity toward catechol and 4-methyl catechol Brassica oleracea
1.14.18.1 21
-
 4-methyl catechol polyphenol oxidase shows the best activity toward catechol and 4-methyl catechol Brassica oleracea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.18.1 51300
-
 SDS-PAGE Brassica oleracea
1.14.18.1 57000
-
 gel filtration Brassica oleracea

Organism

EC Number Organism UniProt Comment Textmining
1.14.18.1 Brassica oleracea
-
 var. botrytis italica
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.18.1 extracted and purified through (NH4)2SO4 precipitation, ion-exchange and gel filtration chromatography Brassica oleracea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.18.1 floret
-
 Brassica oleracea
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.18.1 additional information
-
 purification of PPO from broccoli florets. Specific activity of crude 800, ammonium sulfate precipitation 3058, DEAE 20425, G-50 31430, G-100 37953 U/min/mg protein Brassica oleracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.18.1 4-methylcatechol + 1/2 O2
-
 Brassica oleracea 4-methyl-1,2-benzoquinone + H2O
-
 ?
1.14.18.1 caffeic acid + 1/2 O2
-
 Brassica oleracea caffeoyl quinone + H2O
-
 ?
1.14.18.1 catechol + 1/2 O2
-
 Brassica oleracea 1,2-benzoquinone + H2O
-
 ?
1.14.18.1 chlorogenic acid + 1/2 O2
-
 Brassica oleracea chlorogenoquinone + H2O
-
 ?
1.14.18.1 ferulic acid + O2 + AH2
-
 Brassica oleracea (2E)-3-(3,4-dihydroxy-5-methoxyphenyl)prop-2-enoic acid + H2O + A
-
 ?
1.14.18.1 additional information enzyme catalyzes two distinct reactions: the o-hydroxylation of monophenols to o-diphenols (acts like cresolase (E.C. 1.14.18.1.)) and the oxidation of o-diphenols to o-quinones (acts like catecholase (E.C. 1.10.3.2.)). No activity towards monophenols (tyrosine) and low activity - towards trihydroxyphenol-phloroglucinol Brassica oleracea ?
-
 ?
1.14.18.1 phloroglucinol + 1/2 O2
-
 Brassica oleracea ?
-
 ?
1.14.18.1 vanillin + O2 + AH2
-
 Brassica oleracea 3,4-dihydroxy-5-methoxybenzaldehyde + H2O + A
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.18.1 polyphenol oxidase acts like cresolase and catecholase Brassica oleracea

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.14.18.1 5.7
-
 the optimum pH for broccoli PPO is 5.7 with catechol and 4-methylcatechol as substrates Brassica oleracea

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.14.18.1 0.0317
-
 Sodium sulfate 4-methyl catechol as a substrate Brassica oleracea
1.14.18.1 0.056
-
 ascorbic acid catechol as a substrate Brassica oleracea
1.14.18.1 0.068
-
 EDTA catechol as a substrate Brassica oleracea
1.14.18.1 0.074
-
 Citric acid 4-methyl catechol as a substrate Brassica oleracea
1.14.18.1 0.082
-
 Citric acid catechol as a substrate Brassica oleracea
1.14.18.1 0.082
-
 Sodium sulfate catechol as a substrate Brassica oleracea
1.14.18.1 0.088
-
 ascorbic acid 4-methyl catechol as a substrate Brassica oleracea
1.14.18.1 0.188
-
 EDTA 4-methyl catechol as a substrate Brassica oleracea