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Literature summary extracted from

  • Zameitat, E.; Pierik, A.J.; Zocher, K.; Loeffler, M.
    Dihydroorotate dehydrogenase from Saccharomyces cerevisiae: spectroscopic investigations with the recombinant enzyme throw light on catalytic properties and metabolism of fumarate analogues (2007), FEMS Yeast Res., 7, 897-904.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.98.1 expressed in Escherichia coli strain BL21-RP Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.98.1 L-orotate
-
 Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.98.1 0.016
-
 L-dihydroorotate in 50mM Tris/HCl, 150 mM KCl, 0.1% (v/v) Triton X-100, pH 8, at 30°C Saccharomyces cerevisiae
1.3.98.1 0.115
-
 2,6-dichloroindophenol in 50mM Tris/HCl, 150 mM KCl, 0.1% (v/v) Triton X-100, pH 8, at 30°C Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.98.1 33900
-
 2 * 33900, theoretical molecular mass Saccharomyces cerevisiae
1.3.98.1 67000
-
 SDS-PAGE Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
1.3.98.1 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.98.1 Ni2+-NTA agarose chromatography, Q-Sepharose column chromatography, and Superdex 200 gel filtration Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.98.1 0.06
-
 using crotonate as electron acceptor Saccharomyces cerevisiae
1.3.98.1 0.94
-
 using mesaconate as electron acceptor Saccharomyces cerevisiae
1.3.98.1 1.81
-
 using O2 as electron acceptor Saccharomyces cerevisiae
1.3.98.1 5.1
-
 using fumarate as electron acceptor Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.98.1 L-dihydroorotate + 2,6-dichlorophenolindophenol
-
 Saccharomyces cerevisiae orotate + reduced 2,6-dichlorophenolindophenol
-
 ?
1.3.98.1 L-dihydroorotate + crotonate
-
 Saccharomyces cerevisiae orotate + butanoate
-
 ?
1.3.98.1 L-dihydroorotate + fumarate fumarate is the most effective electron acceptor Saccharomyces cerevisiae orotate + succinate
-
 ?
1.3.98.1 L-dihydroorotate + mesaconate
-
 Saccharomyces cerevisiae orotate + 2-methylbutanedioate
-
 ?
1.3.98.1 L-dihydroorotate + O2
-
 Saccharomyces cerevisiae orotate + H2O2
-
 ?
1.3.98.1 additional information maleate, glutaconate, cis-aconitate, trans-aconitate, and cinnamate do not function as electron acceptor Saccharomyces cerevisiae ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.98.1 homodimer 2 * 33900, theoretical molecular mass Saccharomyces cerevisiae

Synonyms

EC Number Synonyms Comment Organism
1.3.98.1 DHODH
-
 Saccharomyces cerevisiae
1.3.98.1 DHODH-1A
-
 Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.98.1 flavin contains 0.5 mol flavin per mol protein Saccharomyces cerevisiae

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.3.98.1 0.0077
-
 in 50mM Tris/HCl, 150 mM KCl, 0.1% (v/v) Triton X-100, pH 8, at 30°C Saccharomyces cerevisiae L-orotate