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Literature summary extracted from

  • Lisov, A.V.; Zavarzina, A.G.; Zavarzin, A.A.; Leontievsky, A.A.
    Laccases produced by lichens of the order Peltigerales (2007), FEMS Microbiol. Lett., 275, 46-52.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.10.3.2 1,10-phenanthroline 10 mM, complete loss of activity Peltigera aphthosa
1.10.3.2 1,10-phenanthroline 10 mM, complete loss of activity Solorina crocea
1.10.3.2 NaN3 1 mM, complete loss of activity Peltigera aphthosa
1.10.3.2 NaN3 1 mM, complete loss of activity Solorina crocea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.10.3.2 76000
-
 2 * 76000, SDS-PAGE Solorina crocea
1.10.3.2 97000
-
 2 * 97000, SDS-PAGE Peltigera aphthosa
1.10.3.2 165000
-
 gel filtration Peltigera aphthosa
1.10.3.2 175000
-
 gel filtration Solorina crocea

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.2 Peltigera aphthosa
-
-
-
1.10.3.2 Solorina crocea
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.3.2
-
 Solorina crocea
1.10.3.2
-
 Peltigera aphthosa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.10.3.2 thallus
-
 Solorina crocea
-
1.10.3.2 thallus
-
 Peltigera aphthosa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.2 2,2-azino-bis-(3-ethylbenzthiazolin-6-sulfonate) + O2
-
 Solorina crocea ?
-
 ?
1.10.3.2 2,2-azino-bis-(3-ethylbenzthiazolin-6-sulfonate) + O2 56% of the activity with 2,6-dimethoxyphenol Peltigera aphthosa ?
-
 ?
1.10.3.2 2,6-dimethoxyphenol + O2
-
 Peltigera aphthosa ?
-
 ?
1.10.3.2 2,6-dimethoxyphenol + O2 24% of the activity with 2,2-azino-bis-(3-ethylbenzthiazolin-6-sulfonate) Solorina crocea ?
-
 ?
1.10.3.2 2,6-dimethylphenol + O2 1% of the activity with 2,2-azino-bis-(3-ethylbenzthiazolin-6-sulfonate) Solorina crocea ?
-
 ?
1.10.3.2 2,6-dimethylphenol + O2 6% of the activity with 2,6-dimethoxyphenol Peltigera aphthosa ?
-
 ?
1.10.3.2 guaiacol + O2 16% of the activity with 2,6-dimethoxyphenol Peltigera aphthosa ?
-
 ?
1.10.3.2 p-phenylenediamine + O2 46% of the activity with 2,6-dimethoxyphenol Peltigera aphthosa ?
-
 ?
1.10.3.2 p-phenylenediamine + O2 7.5% of the activity with 2,2-azino-bis-(3-ethylbenzthiazolin-6-sulfonate) Solorina crocea ?
-
 ?
1.10.3.2 pyrogallol + O2 19% of the activity with 2,2-azino-bis-(3-ethylbenzthiazolin-6-sulfonate) Solorina crocea ?
-
 ?
1.10.3.2 pyrogallol + O2 57% of the activity with 2,6-dimethoxyphenol Peltigera aphthosa ?
-
 ?
1.10.3.2 syringaldazine + O2 1% of the activity with 2,2-azino-bis-(3-ethylbenzthiazolin-6-sulfonate) Solorina crocea ?
-
 ?
1.10.3.2 syringaldazine + O2 3% of the activity with 2,6-dimethoxyphenol Peltigera aphthosa ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.10.3.2 dimer 2 * 76000, SDS-PAGE Solorina crocea
1.10.3.2 dimer 2 * 97000, SDS-PAGE Peltigera aphthosa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.10.3.2 35
-
 1 h, about 50% loss of activity Solorina crocea
1.10.3.2 35
-
 1 h, about 20% loss of activity Peltigera aphthosa
1.10.3.2 50
-
 10 min, about 80% loss of activity Solorina crocea
1.10.3.2 50
-
 1 h, about 65% loss of activity Peltigera aphthosa
1.10.3.2 60
-
 10 min, about 85% loss of activity Peltigera aphthosa
1.10.3.2 60
-
 10 min, about 85% loss of activity. 40 min, complete inactivation Solorina crocea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.2 2.7
-
 substrate: 2,6-dimethoxyphenol Peltigera aphthosa
1.10.3.2 3.6
-
 substrate: 2,2-azino-bis-(3-ethylbenzthiazolin-6-sulfonate) Peltigera aphthosa
1.10.3.2 4.2
-
 substrate: 2,2-azino-bis-(3-ethylbenzthiazolin-6-sulfonate) or 2,6-dimethoxyphenol Solorina crocea

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.10.3.2 Peltigera aphthosa isoelectric focusing, pH-range: 2.5-5.0
-
 4.2
1.10.3.2 Solorina crocea isoelectric focusing, pH-range: 2.5-5.0
-
 4.8