Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Charalabous, P.; Risk, J.M.; Jenkins, R.; Birss, A.J.; Hart, C.A.; Smalley, J.W.
    Characterization of a bifunctional catalase-peroxidase of Burkholderia cenocepacia (2007), FEMS Immunol. Med. Microbiol., 50, 37-44.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.11.1.6 expression in Escherichia coli Burkholderia cenocepacia
1.11.1.21 expressed in Escherichia coli BL21 Star One Shot cells Burkholderia cenocepacia

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.11.1.6 additional information not inhibited by exposure of cells to 3-amino-1,2,4-triazole Burkholderia cenocepacia
1.11.1.21 additional information the peroxidase activity of the enzyme against 3,3',5,5'-tetramethylbenzidine, 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid), and o-dianisidine is unaffected by the inhibitors isonicotinic acid and niacinamide (all 20 mM), the enzyme is not inhibited by the specific monofunctional catalase inhibitor 3-amino-1,2,4,-triazole Burkholderia cenocepacia

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.1.6 80000
-
 x * 97000, SDS-PAGE of mature enzyme, x * 80000, SDS-PAGE of recombinant protein Burkholderia cenocepacia
1.11.1.6 97000
-
 x * 97000, SDS-PAGE of mature enzyme, x * 80000, SDS-PAGE of recombinant protein Burkholderia cenocepacia
1.11.1.21 80000
-
 x * 80000, recombinant enzyme, SDS-PAGE Burkholderia cenocepacia
1.11.1.21 80500
-
 x * 80500, calculated from amino acid sequence Burkholderia cenocepacia
1.11.1.21 97000
-
 x * 97000, mature catalase-peroxidase, SDS-PAGE Burkholderia cenocepacia

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.6 Burkholderia cenocepacia Q4F6N6 bifunctional catalase-peroxidase KatG
-
1.11.1.21 Burkholderia cenocepacia
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.21 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O2
-
 Burkholderia cenocepacia oxidized 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O
-
 ?
1.11.1.21 3,3',5,5'-tetramethylbenzidine + H2O2
-
 Burkholderia cenocepacia oxidized 3,3',5,5'-tetramethylbenzidine + H2O
-
 ?
1.11.1.21 H2O2
-
 Burkholderia cenocepacia O2 + H2O
-
 ?
1.11.1.21 additional information guaicol (methoxyphenol) and 2-chloronaphthol are not peroxidized by the enzyme Burkholderia cenocepacia ?
-
 ?
1.11.1.21 o-dianisidine + H2O2
-
 Burkholderia cenocepacia oxidized o-dianisidine + H2O
-
 ?
1.11.1.21 pyrogallol + H2O2
-
 Burkholderia cenocepacia ? + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.11.1.6 ? x * 97000, SDS-PAGE of mature enzyme, x * 80000, SDS-PAGE of recombinant protein Burkholderia cenocepacia
1.11.1.21 ? x * 80000, recombinant enzyme, SDS-PAGE Burkholderia cenocepacia
1.11.1.21 ? x * 80500, calculated from amino acid sequence Burkholderia cenocepacia
1.11.1.21 ? x * 97000, mature catalase-peroxidase, SDS-PAGE Burkholderia cenocepacia

Synonyms

EC Number Synonyms Comment Organism
1.11.1.21 KatG
-
 Burkholderia cenocepacia

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.1.6 5.5 6 both catalase and peroxidase activity Burkholderia cenocepacia
1.11.1.21 5.5
-
 pH optimum for peroxidase activity Burkholderia cenocepacia
1.11.1.21 6
-
 pH optimum for catalase activity Burkholderia cenocepacia

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.11.1.6 additional information
-
 no catalytic activity at pH 9.0 Burkholderia cenocepacia
1.11.1.6 5.5 8.5
-
 Burkholderia cenocepacia
1.11.1.21 5.5 8.5 pH range for both catalase and peroxidase activities, little or no activity is seen at pH 9.0 Burkholderia cenocepacia

Cofactor

EC Number Cofactor Comment Organism Structure
1.11.1.21 heme
-
 Burkholderia cenocepacia