EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.4.1.7 | D338N | site-directed mutagenesis of a fructose-binding residue, the mutant shows 7000fold reduced activity compared to the wild-type enzyme due to disruption of steps where fructose departs or attacks | Leuconostoc mesenteroides |
2.4.1.7 | R137A | site-directed mutagenesis of a phosphate-binding residue, the mutant shows 60fold reduced activity compared to the wild-type enzyme due to disruption of steps where fructose departs or attacks | Leuconostoc mesenteroides |
2.4.1.7 | Y340A | site-directed mutagenesis of a phosphate-binding residue, the mutant shows 2500fold reduced activity compared to the wild-type enzyme due to disruption of steps where fructose departs or attacks | Leuconostoc mesenteroides |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.1.7 | additional information | - |
additional information | kinetics of recombinant wild-type and mutant enzymes, overview | Leuconostoc mesenteroides |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.7 | sucrose + phosphate | Leuconostoc mesenteroides | - |
D-fructose + alpha-D-glucose 1-phosphate | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.1.7 | Leuconostoc mesenteroides | - |
- |
- |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
2.4.1.7 | sucrose + phosphate = D-fructose + alpha-D-glucose 1-phosphate | reaction mechanism | Leuconostoc mesenteroides |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.4.1.7 | additional information | - |
- |
Leuconostoc mesenteroides |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.7 | alpha-D-glucose 1-phosphate + arsenate | - |
Leuconostoc mesenteroides | ? | - |
? | |
2.4.1.7 | sucrose + phosphate | - |
Leuconostoc mesenteroides | D-fructose + alpha-D-glucose 1-phosphate | - |
r | |
2.4.1.7 | sucrose + phosphate | catalytic mechanisms of wild-type and mutant enzymes, overview | Leuconostoc mesenteroides | D-fructose + alpha-D-glucose 1-phosphate | - |
r |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.4.1.7 | SPase | - |
Leuconostoc mesenteroides |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.1.7 | 30 | - |
assay at | Leuconostoc mesenteroides |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.1.7 | 72 | - |
D-fructose | pH 7.0, 30°C, synthesis, wild-type enzyme | Leuconostoc mesenteroides | |
2.4.1.7 | 91 | - |
alpha-D-glucose 1-phosphate | pH 7.0, 30°C, synthesis, wild-type enzyme | Leuconostoc mesenteroides | |
2.4.1.7 | 170 | - |
arsenate | pH 7.0, 30°C, arsenolysis, wild-type enzyme | Leuconostoc mesenteroides | |
2.4.1.7 | 170 | - |
sucrose | pH 7.0, 30°C, phosphorolysis, wild-type enzyme | Leuconostoc mesenteroides | |
2.4.1.7 | 200 | - |
phosphate | pH 7.0, 30°C, phosphorolysis, wild-type enzyme | Leuconostoc mesenteroides | |
2.4.1.7 | 260 | - |
alpha-D-glucose 1-phosphate | pH 7.0, 30°C, arsenolysis, wild-type enzyme | Leuconostoc mesenteroides |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.4.1.7 | 7 | - |
assay at | Leuconostoc mesenteroides |