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Literature summary extracted from
- Identification of the molecular basis for the functional difference between flavonoid 3'-hydroxylase and flavonoid 3',5'-hydroxylase (2007), FEBS Lett., 581, 3429-3434.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.14.14.81 | expression of wild-type enzyme and chimeric mutants in yeast strain INVSc 1 microsomes | Osteospermum hybrid cultivar |
| 1.14.14.82 | expression of wild-type enzyme and chimeric mutants in yeast strain INVSc 1 microsomes | Gerbera hybrid cultivar |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 1.14.14.81 | F484Y/S487T | site-directed mutagenesis, altered substrate specificity compared to the wild-type enzyme | Osteospermum hybrid cultivar |
| 1.14.14.81 | F48Y | site-directed mutagenesis, altered substrate specificity compared to the wild-type enzyme | Osteospermum hybrid cultivar |
| 1.14.14.81 | additional information | generation of chimeric constructs consisting of cDNA fragments of Osteospermum hybrida F3'5'H and Gerbera hybrida F3'H, EC 1.14.13.21, overview | Osteospermum hybrid cultivar |
| 1.14.14.81 | S487A | site-directed mutagenesis, altered substrate specificity compared to the wild-type enzyme | Osteospermum hybrid cultivar |
| 1.14.14.81 | S487Y | site-directed mutagenesis, altered substrate specificity compared to the wild-type enzyme | Osteospermum hybrid cultivar |
| 1.14.14.82 | additional information | generation of chimeric constructs consisting of cDNA fragments of Gerbera hybrida F3'H and Osteospermum hybrida F3'5'H, EC 1.14.13.88, overview | Gerbera hybrid cultivar |
| 1.14.14.82 | T484F | site-directed mutagenesis, altered substrate specificity compared to the wild-type enzyme | Gerbera hybrid cultivar |
| 1.14.14.82 | T487A | site-directed mutagenesis, altered substrate specificity compared to the wild-type enzyme | Gerbera hybrid cultivar |
| 1.14.14.82 | T487S | site-directed mutagenesis, a conservative Thr to Ser exchange at position 487 conferred additional 5'-hydroxylation activity to recombinant Gerbera hybrida F3'H | Gerbera hybrid cultivar |
| 1.14.14.82 | Y484F/T487S | site-directed mutagenesis, altered substrate specificity compared to the wild-type enzyme | Gerbera hybrid cultivar |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.14.81 | Fe2+ | a cytochrome P450 enzyme | Osteospermum hybrid cultivar |  |
| 1.14.14.82 | Fe2+ | a cytochrome P450 enzyme | Gerbera hybrid cultivar | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.14.81 | apigenin + [reduced NADPH-hemoprotein reductase] + O2 | Osteospermum hybrid cultivar | - |
? | - |
? | |
| 1.14.14.81 | dihydrokaempferol + 2 [reduced NADPH-hemoprotein reductase] + 2 O2 | Osteospermum hybrid cultivar | - |
dihydromyricetin + 2 [oxidized NADPH-hemoprotein reductase] + 2 H2O | - |
? | |
| 1.14.14.81 | additional information | Osteospermum hybrid cultivar | substrate specificity of F3'H and the F3'5'H, flavonoid 3',5'-hydroxylase, EC 1.14.13.88, evolutionary derived from F3'H, is determined near the N-terminal end and the functional difference between these two enzymes near the C-terminal end, relatively few amino acids exchanges are required for the evolutionary extension of 3'- to 3',5'-hydroxylation activity, overview | ? | - |
? | |
| 1.14.14.81 | naringenin + 2 [reduced NADPH-hemoprotein reductase] + 2 O2 | Osteospermum hybrid cultivar | - |
(2S)-5,7,3',4',5'-pentahydroxyflavanone + 2 [oxidized NADPH-hemoprotein reductase] + 2 H2O | - |
? | |
| 1.14.14.82 | dihydrokaempferol + [reduced NADPH-hemoprotein reductase] + O2 | Gerbera hybrid cultivar | - |
dihydroquercetin + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.14.82 | additional information | Gerbera hybrid cultivar | substrate specificity of F3'H and the F3'5'H, flavonoid 3',5'-hydroxylase, EC 1.14.13.88, evolutionary derived from F3'H, is determined near the N-terminal end and the functional difference between these two enzymes near the C-terminal end, relatively few amino acids exchanges are required for the evolutionary extension of 3'- to 3',5'-hydroxylation activity, overview | ? | - |
? | |
| 1.14.14.82 | naringenin + [reduced NADPH-hemoprotein reductase] + O2 | Gerbera hybrid cultivar | - |
eriodyctiol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.14.81 | Osteospermum hybrid cultivar | Q304Q4 | - |
- |
| 1.14.14.82 | Gerbera hybrid cultivar | Q38L00 | - |
- |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.81 | additional information | - |
activities of recombinant chimeric mutant enzymes, overview | Osteospermum hybrid cultivar |
| 1.14.14.81 | 0.0000026 | - |
recombinant wild-type F3'5'H | Osteospermum hybrid cultivar |
| 1.14.14.82 | additional information | - |
activities of recombinant chimeric mutant enzymes, overview | Gerbera hybrid cultivar |
| 1.14.14.82 | 0.000003 | - |
recombinant wild-type F3'H | Gerbera hybrid cultivar |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.14.81 | apigenin + [reduced NADPH-hemoprotein reductase] + O2 | - |
Osteospermum hybrid cultivar | ? | - |
? | |
| 1.14.14.81 | dihydrokaempferol + 2 [reduced NADPH-hemoprotein reductase] + 2 O2 | - |
Osteospermum hybrid cultivar | dihydromyricetin + 2 [oxidized NADPH-hemoprotein reductase] + 2 H2O | - |
? | |
| 1.14.14.81 | dihydrokaempferol + 2 [reduced NADPH-hemoprotein reductase] + 2 O2 | preferred substrate of the wild-type enzyme | Osteospermum hybrid cultivar | dihydromyricetin + 2 [oxidized NADPH-hemoprotein reductase] + 2 H2O | - |
? | |
| 1.14.14.81 | additional information | substrate specificity of F3'H and the F3'5'H, flavonoid 3',5'-hydroxylase, EC 1.14.13.88, evolutionary derived from F3'H, is determined near the N-terminal end and the functional difference between these two enzymes near the C-terminal end, relatively few amino acids exchanges are required for the evolutionary extension of 3'- to 3',5'-hydroxylation activity, overview | Osteospermum hybrid cultivar | ? | - |
? | |
| 1.14.14.81 | naringenin + 2 [reduced NADPH-hemoprotein reductase] + 2 O2 | - |
Osteospermum hybrid cultivar | (2S)-5,7,3',4',5'-pentahydroxyflavanone + 2 [oxidized NADPH-hemoprotein reductase] + 2 H2O | - |
? | |
| 1.14.14.82 | dihydrokaempferol + [reduced NADPH-hemoprotein reductase] + O2 | - |
Gerbera hybrid cultivar | dihydroquercetin + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.14.82 | additional information | substrate specificity of F3'H and the F3'5'H, flavonoid 3',5'-hydroxylase, EC 1.14.13.88, evolutionary derived from F3'H, is determined near the N-terminal end and the functional difference between these two enzymes near the C-terminal end, relatively few amino acids exchanges are required for the evolutionary extension of 3'- to 3',5'-hydroxylation activity, overview | Gerbera hybrid cultivar | ? | - |
? | |
| 1.14.14.82 | naringenin + [reduced NADPH-hemoprotein reductase] + O2 | - |
Gerbera hybrid cultivar | eriodyctiol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.14.82 | naringenin + [reduced NADPH-hemoprotein reductase] + O2 | preferred substrate of the wild-type enzyme | Gerbera hybrid cultivar | eriodyctiol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.14.81 | F3'5'H | - |
Osteospermum hybrid cultivar |
| 1.14.14.81 | flavonoid 3',5'-hydroxylase | - |
Osteospermum hybrid cultivar |
| 1.14.14.81 | More | the enzyme belongs to the subfamilies CYP75A of the superfamily of cytochrome P450-dependent monooxygenases | Osteospermum hybrid cultivar |
| 1.14.14.82 | F3'H | - |
Gerbera hybrid cultivar |
| 1.14.14.82 | flavonoid 3'-hydroxylase | - |
Gerbera hybrid cultivar |
| 1.14.14.82 | More | the enzyme belongs to the subfamilies CYP75B of the superfamily of cytochrome P450-dependent monooxygenases | Gerbera hybrid cultivar |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.81 | 30 | - |
assay at | Osteospermum hybrid cultivar |
| 1.14.14.82 | 30 | - |
assay at | Gerbera hybrid cultivar |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.81 | 7.5 | - |
assay at | Osteospermum hybrid cultivar |
| 1.14.14.82 | 7.5 | - |
assay at | Gerbera hybrid cultivar |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.14.81 | cytochrome P450 | a cytochrome P450 enzyme | Osteospermum hybrid cultivar | |
| 1.14.14.82 | cytochrome | a cytochrome P450 enzyme | Gerbera hybrid cultivar |
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