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Literature summary extracted from

  • Koivistoinen, O.M.; Hilditch, S.; Voutilainen, S.P.; Boer, H.; Penttilae, M.; Richard, P.
    Identification in the yeast Pichia stipitis of the first L-rhamnose-1-dehydrogenase gene (2008), FEBS J., 275, 2482-2488.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.173 additional information enzyme transcription in Pichia stipitis is induced during growth on L-rhamnose but not on other carbon sources Scheffersomyces stipitis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.173 gene RHA1, DNA and amino acid sequence determination and analysis, expression in Saccharomyces cereviaie Scheffersomyces stipitis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.173 additional information D-glucose suppresses the enzyme Scheffersomyces stipitis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.173 additional information
-
 additional information substrate specificity, overview Scheffersomyces stipitis
1.1.1.173 1.5
-
 L-rhamnose pH 8.0, 30°C, recombinant enzyme Scheffersomyces stipitis
1.1.1.173 5
-
 L-Lyxose pH 8.0, 30°C, recombinant enzyme Scheffersomyces stipitis
1.1.1.173 25
-
 L-Mannose pH 8.0, 30°C, recombinant enzyme Scheffersomyces stipitis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.173 30000
-
 x * 30000, recombinant enzyme, SDS-PAGE Scheffersomyces stipitis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.173 L-rhamnose + NAD+ Scheffersomyces stipitis the enzyme is involved in the rhamnose metabolism, overview L-rhamno-1,4-lactone + NADH
-
 r
1.1.1.173 L-rhamnose + NAD+ Scheffersomyces stipitis NRC 5568 the enzyme is involved in the rhamnose metabolism, overview L-rhamno-1,4-lactone + NADH
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.173 Scheffersomyces stipitis
-
 gene RHA1
-
1.1.1.173 Scheffersomyces stipitis NRC 5568
-
 gene RHA1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.173 recombinant enzyme in analytical amounts from Saccharomyces cerevisiae by anion exchange chromatography, and native and SDS-PAGE, tagging of the enzyme strongly affects its activity, overview Scheffersomyces stipitis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.173 0.6
-
 purified recombinant enzyme Scheffersomyces stipitis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.173 L-lyxose + NAD+
-
 Scheffersomyces stipitis ? + NADH
-
 ?
1.1.1.173 L-lyxose + NAD+
-
 Scheffersomyces stipitis NRC 5568 ? + NADH
-
 ?
1.1.1.173 L-mannose + NAD+
-
 Scheffersomyces stipitis ? + NADH
-
 ?
1.1.1.173 L-mannose + NAD+
-
 Scheffersomyces stipitis NRC 5568 ? + NADH
-
 ?
1.1.1.173 L-rhamnose + NAD+ the enzyme is involved in the rhamnose metabolism, overview Scheffersomyces stipitis L-rhamno-1,4-lactone + NADH
-
 r
1.1.1.173 L-rhamnose + NAD+ the heterologous protein shows the highest activity and affinity with L-rhamnose and a lower activity and affinity with L-mannose and L-lyxose Scheffersomyces stipitis L-rhamno-1,4-lactone + NADH
-
 r
1.1.1.173 L-rhamnose + NAD+ the enzyme is involved in the rhamnose metabolism, overview Scheffersomyces stipitis NRC 5568 L-rhamno-1,4-lactone + NADH
-
 r
1.1.1.173 L-rhamnose + NAD+ the heterologous protein shows the highest activity and affinity with L-rhamnose and a lower activity and affinity with L-mannose and L-lyxose Scheffersomyces stipitis NRC 5568 L-rhamno-1,4-lactone + NADH
-
 r
1.1.1.173 additional information substrate specificity, overview Scheffersomyces stipitis ?
-
 ?
1.1.1.173 additional information substrate specificity, overview Scheffersomyces stipitis NRC 5568 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.173 ? x * 30000, recombinant enzyme, SDS-PAGE Scheffersomyces stipitis

Synonyms

EC Number Synonyms Comment Organism
1.1.1.173 L-rhamnose-1-dehydrogenase
-
 Scheffersomyces stipitis
1.1.1.173 NAD-utilizing L-rhamnose 1-dehydrogenase
-
 Scheffersomyces stipitis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.173 30
-
 assay at Scheffersomyces stipitis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.173 8
-
-
 Scheffersomyces stipitis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.173 additional information no activity with NADPH Scheffersomyces stipitis
1.1.1.173 NAD+ strictly dependent on Scheffersomyces stipitis
1.1.1.173 NADH strictly dependent on Scheffersomyces stipitis