Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Scholz, J.; Toska, K.; Luborzewski, A.; Maass, A.; Schuenemann, V.; Haavik, J.; Moser, A.
    Endogenous tetrahydroisoquinolines associated with Parkinsons disease mimic the feedback inhibition of tyrosine hydroxylase by catecholamines (2008), FEBS J., 275, 2109-2121.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.14.16.2 catecholamine binding improves tyrosine hydroxylase's resistance to proteolysis Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.16.2 Catecholamines e.g. dopamine, feedback inhibition, phosphorylation by protein kinase A at Ser40, the most prominent of these regulatory sites, increases the dissociation rate of bound catecholamine inhibitors Homo sapiens
1.14.16.2 dopamine binds to the iron ion at the active site Homo sapiens
1.14.16.2 additional information no inhibition by nonhydroxylated N-methyl-1,2,3,4-tetrahydroisoquinoline Homo sapiens
1.14.16.2 N-methyl-norsalsolinol noncompetitive with respect to L-tyrosine, N-methyl-norsalsolinol and related tetrahydroisoquinolines accumulate in the nigrostriatal system of the human brain and are increased in the cerebrospinal fluid of patients with Parkinson’s disease Homo sapiens
1.14.16.2 N-methyl-salsolinol
-
 Homo sapiens
1.14.16.2 norsalsolinol N-methyl-norsalsolinol and related tetrahydroisoquinolines accumulate in the nigrostriatal system of the human brain and are increased in the cerebrospinal fluid of patients with Parkinson’s disease Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.16.2 Fe2+ localized at the active site, the ferric is the inactive state, modelling of catecholamine and 6,7-dihydroxylated tetrahydroisoquinoline inhibitor docking to the active site Fe2+, overview Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.16.2 L-tyrosine + tetrahydrobiopterin + O2 Homo sapiens key enzyme in dopamine synthesis, negative catecholamine end-product regulation, overview 3,4-dihydroxy-L-phenylalanine + 4a-hydroxytetrahydrobiopterin + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.16.2 Homo sapiens
-
 isozymes TH1 and TH4
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.14.16.2 phosphoprotein enzyme activation by reversible phosphorylation, phosphorylation by protein kinase A at Ser40, the most prominent of these regulatory sites, increases the dissociation rate of bound catecholamine feedback inhibitors Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.16.2 brain
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.16.2 additional information
-
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.16.2 L-tyrosine + tetrahydrobiopterin + O2
-
 Homo sapiens 3,4-dihydroxy-L-phenylalanine + 4a-hydroxytetrahydrobiopterin + H2O
-
 ?
1.14.16.2 L-tyrosine + tetrahydrobiopterin + O2 key enzyme in dopamine synthesis, negative catecholamine end-product regulation, overview Homo sapiens 3,4-dihydroxy-L-phenylalanine + 4a-hydroxytetrahydrobiopterin + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.16.2 tyrosine hydroxylase
-
 Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.14.16.2 37
-
 5 h, stable, catecholamine, N-methyl-norsalsolinol, or norsalsolinol binding increases the thermal stability of tyrosine hydroxylase Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.16.2 tetrahydrobiopterin
-
 Homo sapiens

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.14.16.2 0.0002
-
-
 Homo sapiens dopamine
1.14.16.2 0.0003
-
-
 Homo sapiens N-methyl-norsalsolinol
1.14.16.2 0.004
-
-
 Homo sapiens N-methyl-salsolinol
1.14.16.2 0.01
-
-
 Homo sapiens norsalsolinol