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Literature summary extracted from

  • Limauro, D.; Pedone, E.; Galdi, I.; Bartolucci, S.
    Peroxiredoxins as cellular guardians in Sulfolobus solfataricus: characterization of Bcp1, Bcp3 and Bcp4 (2008), FEBS J., 275, 2067-2077.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.11.1.24 expressed in Escherichia coli Saccharolobus solfataricus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.1.24 16980
-
 Bcp3, calculated from amino acid sequence Saccharolobus solfataricus
1.11.1.24 17460
-
 Bcp1, calculated from amino acid sequence Saccharolobus solfataricus
1.11.1.24 17460
-
 Bcp4, calculated from amino acid sequence Saccharolobus solfataricus
1.11.1.24 18000
-
 Bcp1, SDS-PAGE Saccharolobus solfataricus
1.11.1.24 18040
-
 Bcp3, MALDI-TOF mass spectrometry Saccharolobus solfataricus
1.11.1.24 18530
-
 Bcp1, MALDI-TOF mass spectrometry Saccharolobus solfataricus
1.11.1.24 18530
-
 Bcp4, MALDI-TOF mass spectrometry Saccharolobus solfataricus

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
1.11.1.24 urea Bcp4 retains 50% of its activity after a 30 min incubation in 6 M urea Saccharolobus solfataricus

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.24 Saccharolobus solfataricus Q97VL0
-
-
1.11.1.24 Saccharolobus solfataricus Q97WG5
-
-
1.11.1.24 Saccharolobus solfataricus Q97WP9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.24 HisTrap HP column chromatography, Resource S column chromatography, and Resource Q column chromatography Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.24 H2O2 + reduced thioredoxin H2O2 is the preferred substrate compared to tert-butyl hydroperoxide Saccharolobus solfataricus H2O + oxidized thioredoxin
-
 ?
1.11.1.24 tert-butyl hydroperoxide + reduced thioredoxin H2O2 is the preferred substrate compared to tert-butyl hydroperoxide Saccharolobus solfataricus tert-butanol + oxidized thioredoxin + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.11.1.24 dimer Bcp1, gel filtration Saccharolobus solfataricus
1.11.1.24 dimer Bcp3, gel filtration Saccharolobus solfataricus
1.11.1.24 monomer Bcp4, gel filtration Saccharolobus solfataricus

Synonyms

EC Number Synonyms Comment Organism
1.11.1.24 bacterioferritin comigratory protein
-
 Saccharolobus solfataricus
1.11.1.24 Bcp1 the isozyme is classified as a 2-Cys peroxiredoxin Saccharolobus solfataricus
1.11.1.24 Bcp3 the isozyme is classified as a 1-Cys peroxiredoxin Saccharolobus solfataricus
1.11.1.24 Bcp4 the isozyme is classified as a 2-Cys peroxiredoxin Saccharolobus solfataricus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.11.1.24 85
-
-
 Saccharolobus solfataricus
1.11.1.24 85
-
 Bcp1 and Bcp3 Saccharolobus solfataricus
1.11.1.24 95 100
-
 Saccharolobus solfataricus
1.11.1.24 95 100 Bcp4 Saccharolobus solfataricus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.11.1.24 80 100 Bcp1 shows no loss of activity after 2 h incubation at 80°C, displays a half-life of 2 h at 95°C but after a 60 min incubation at 100°C its relative activity drops to 20% of the starting value Saccharolobus solfataricus
1.11.1.24 80 100 Bcp3 shows no loss of activity after 2 h incubation at 80°C, after 2 h at 95°C relative activity drops to 60% Saccharolobus solfataricus
1.11.1.24 80 100 Bcp4 shows no loss of activity after 2 h incubation at 80°C, after 2 h at 95°C relative activity drops to 92% Saccharolobus solfataricus

Cofactor

EC Number Cofactor Comment Organism Structure
1.11.1.24 thioredoxin
-
 Saccharolobus solfataricus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.11.1.24 Saccharolobus solfataricus Bcp4, calculated from amino acid sequence
-
 7.65
1.11.1.24 Saccharolobus solfataricus Bcp1, calculated from amino acid sequence
-
 7.73
1.11.1.24 Saccharolobus solfataricus Bcp3, calculated from amino acid sequence
-
 8.84