Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Tanka-Salamon, A.; Tenekedjiev, K.; Machovich, R.; Kolev, K.
    Suppressed catalytic efficiency of plasmin in the presence of long-chain fatty acids. Identification of kinetic parameters from continuous enzymatic assay with Monte Carlo stimulation (2008), FEBS J., 275, 1274-1282.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.21.7 arachidonate
-
 synthetic construct
3.4.21.7 oleate
-
 synthetic construct
3.4.21.7 stearate
-
 synthetic construct

Protein Variants

EC Number Protein Variants Comment Organism
3.4.21.7 additional information truncated plasmin variants. Miniplasmin (des-kringle 1-4 plasmin) contains the kringle 5 and the catalytic domain of plasmin, whereas microplasmin (des-kringle 1-5 plasmin) is composed of the catalytic domain only. Miniplasmin (des-kringle 1-4 plasmin) is as sensitive to fatty acids as plasmin, whereas the activity of microplasmin (des-kringle 1-5 plasmin) is not affected synthetic construct

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.7 arachidonate
-
 synthetic construct
3.4.21.7 oleate
-
 synthetic construct
3.4.21.7 stearate
-
 synthetic construct

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.7 0.00589
-
 D-norleucyl-hexahydrotyrosyl-lysine-p-nitroanilide in the absence of fatty acids synthetic construct
3.4.21.7 0.00817
-
 D-norleucyl-hexahydrotyrosyl-lysine-p-nitroanilide in the presence of 0.065 mM stearate synthetic construct
3.4.21.7 0.01133
-
 D-norleucyl-hexahydrotyrosyl-lysine-p-nitroanilide in the presence of 0.115 mM stearate synthetic construct
3.4.21.7 0.01258
-
 D-norleucyl-hexahydrotyrosyl-lysine-p-nitroanilide in the presence of 0.01 mM oleate synthetic construct
3.4.21.7 0.02009
-
 D-norleucyl-hexahydrotyrosyl-lysine-p-nitroanilide in the presence of 0.025 mM oleate synthetic construct
3.4.21.7 0.02337
-
 D-norleucyl-hexahydrotyrosyl-lysine-p-nitroanilide in the presence of 0.175 mM stearate synthetic construct
3.4.21.7 0.02371
-
 D-norleucyl-hexahydrotyrosyl-lysine-p-nitroanilide in the presence of 0.01 mM arachidonate synthetic construct
3.4.21.7 0.02749
-
 D-norleucyl-hexahydrotyrosyl-lysine-p-nitroanilide in the presence of 0.045 mM oleate synthetic construct
3.4.21.7 0.04265
-
 D-norleucyl-hexahydrotyrosyl-lysine-p-nitroanilide in the presence of 0.025 mM arachidonate synthetic construct
3.4.21.7 0.05751
-
 D-norleucyl-hexahydrotyrosyl-lysine-p-nitroanilide in the presence of 0.045 mM arachidonate synthetic construct
3.4.21.7 0.05985
-
 D-norleucyl-hexahydrotyrosyl-lysine-p-nitroanilide in the presence of 0.065 mM arachidonate synthetic construct
3.4.21.7 0.07296
-
 D-norleucyl-hexahydrotyrosyl-lysine-p-nitroanilide in the presence of 0.23 mM stearate synthetic construct
3.4.21.7 0.1311
-
 D-norleucyl-hexahydrotyrosyl-lysine-p-nitroanilide in the presence of 0.065 mM oleate synthetic construct

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.7 synthetic construct
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.7 D-norleucyl-hexahydrotyrosyl-lysine-p-nitroanilide + H2O
-
 synthetic construct ?
-
 ?

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.21.7 0.0002
-
 stearate in the presence of 0.23 mM stearate synthetic construct
3.4.21.7 0.0007
-
 stearate in the presence of 0.175 mM stearate synthetic construct
3.4.21.7 0.0012
-
 stearate in the presence of 0.115 mM stearate synthetic construct
3.4.21.7 0.0025
-
 stearate in the presence of 0.065 mM stearate synthetic construct