BRENDA - Enzyme Database show

Caenorhabditis elegans expresses a functional ArsA

Tseng, Y.Y.; Yu, C.W.; Liao, V.H.; FEBS J. 274, 2566-2572 (2007)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
7.3.2.7
expression of the enzyme as maltose-binding-protein fusion protein in Escherichia coli
Caenorhabditis elegans
Engineering
EC Number
Amino acid exchange
Commentary
Organism
7.3.2.7
additional information
asna-1-mutant nematodes are more sensitive to As(III) and Sb(III) toxicity than are wild-type animals
Caenorhabditis elegans
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
7.3.2.7
0.00004
-
Sb3+
-
Caenorhabditis elegans
7.3.2.7
0.021
-
ATP
pH 7.4, 37°C, wild-type enzyme in absence of Sb3+
Caenorhabditis elegans
7.3.2.7
0.026
-
ATP
pH 7.4, 37°C, wild-type enzyme in presence of Sb3+
Caenorhabditis elegans
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
7.3.2.7
As3+
activates the ATPase activity of the ASNA-1 protein 2.0fold, Km is 3.54 mM at ATP saturation
Caenorhabditis elegans
7.3.2.7
Mg2+
required
Caenorhabditis elegans
7.3.2.7
Sb3+
activates the ATPase activity of the ASNA-1 protein 4.6fold, Km is 0.04 mM at ATP saturation
Caenorhabditis elegans
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
7.3.2.7
ATP + H2O + arsenite/in
Caenorhabditis elegans
ASNA-1 , encoding a functional ArsA ATPase, is critical for As(III) and Sb(III) tolerance in the intact organism, the enzyme does not provide resistance to other metals, e.g. Cu2+ or Pb2+, overview
ADP + phosphate + arsenite/out
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
7.3.2.7
Caenorhabditis elegans
P30632
gene asna-1
-
Purification (Commentary)
EC Number
Commentary
Organism
7.3.2.7
recombinant maltose-binding-protein fusion protein from Escherichia coli by amylose affinity chromatography
Caenorhabditis elegans
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
7.3.2.7
28.87
-
purified recombinant MBP-fusion enzyme stimulated by 0.5 mM Sb3+
Caenorhabditis elegans
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
7.3.2.7
ATP + H2O + arsenite/in
-
686653
Caenorhabditis elegans
ADP + phosphate + arsenite/out
-
-
-
?
7.3.2.7
ATP + H2O + arsenite/in
ASNA-1 , encoding a functional ArsA ATPase, is critical for As(III) and Sb(III) tolerance in the intact organism, the enzyme does not provide resistance to other metals, e.g. Cu2+ or Pb2+, overview
686653
Caenorhabditis elegans
ADP + phosphate + arsenite/out
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
7.3.2.7
37
-
assay at
Caenorhabditis elegans
Temperature Range [°C]
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
7.3.2.7
25
37
-
Caenorhabditis elegans
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.3.2.7
7.4
-
assay at
Caenorhabditis elegans
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
7.3.2.7
expression of the enzyme as maltose-binding-protein fusion protein in Escherichia coli
Caenorhabditis elegans
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
7.3.2.7
additional information
asna-1-mutant nematodes are more sensitive to As(III) and Sb(III) toxicity than are wild-type animals
Caenorhabditis elegans
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
7.3.2.7
0.00004
-
Sb3+
-
Caenorhabditis elegans
7.3.2.7
0.021
-
ATP
pH 7.4, 37°C, wild-type enzyme in absence of Sb3+
Caenorhabditis elegans
7.3.2.7
0.026
-
ATP
pH 7.4, 37°C, wild-type enzyme in presence of Sb3+
Caenorhabditis elegans
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
7.3.2.7
As3+
activates the ATPase activity of the ASNA-1 protein 2.0fold, Km is 3.54 mM at ATP saturation
Caenorhabditis elegans
7.3.2.7
Mg2+
required
Caenorhabditis elegans
7.3.2.7
Sb3+
activates the ATPase activity of the ASNA-1 protein 4.6fold, Km is 0.04 mM at ATP saturation
Caenorhabditis elegans
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
7.3.2.7
ATP + H2O + arsenite/in
Caenorhabditis elegans
ASNA-1 , encoding a functional ArsA ATPase, is critical for As(III) and Sb(III) tolerance in the intact organism, the enzyme does not provide resistance to other metals, e.g. Cu2+ or Pb2+, overview
ADP + phosphate + arsenite/out
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
7.3.2.7
recombinant maltose-binding-protein fusion protein from Escherichia coli by amylose affinity chromatography
Caenorhabditis elegans
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
7.3.2.7
28.87
-
purified recombinant MBP-fusion enzyme stimulated by 0.5 mM Sb3+
Caenorhabditis elegans
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
7.3.2.7
ATP + H2O + arsenite/in
-
686653
Caenorhabditis elegans
ADP + phosphate + arsenite/out
-
-
-
?
7.3.2.7
ATP + H2O + arsenite/in
ASNA-1 , encoding a functional ArsA ATPase, is critical for As(III) and Sb(III) tolerance in the intact organism, the enzyme does not provide resistance to other metals, e.g. Cu2+ or Pb2+, overview
686653
Caenorhabditis elegans
ADP + phosphate + arsenite/out
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
7.3.2.7
37
-
assay at
Caenorhabditis elegans
Temperature Range [°C] (protein specific)
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
7.3.2.7
25
37
-
Caenorhabditis elegans
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.3.2.7
7.4
-
assay at
Caenorhabditis elegans