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Literature summary extracted from

  • Tseng, Y.Y.; Yu, C.W.; Liao, V.H.
    Caenorhabditis elegans expresses a functional ArsA (2007), FEBS J., 274, 2566-2572.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
7.3.2.7 expression of the enzyme as maltose-binding-protein fusion protein in Escherichia coli Caenorhabditis elegans

Protein Variants

EC Number Protein Variants Comment Organism
7.3.2.7 additional information asna-1-mutant nematodes are more sensitive to As(III) and Sb(III) toxicity than are wild-type animals Caenorhabditis elegans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
7.3.2.7 0.00004
-
Sb3+
-
Caenorhabditis elegans
7.3.2.7 0.021
-
ATP pH 7.4, 37°C, wild-type enzyme in absence of Sb3+ Caenorhabditis elegans
7.3.2.7 0.026
-
ATP pH 7.4, 37°C, wild-type enzyme in presence of Sb3+ Caenorhabditis elegans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
7.3.2.7 As3+ activates the ATPase activity of the ASNA-1 protein 2.0fold, Km is 3.54 mM at ATP saturation Caenorhabditis elegans
7.3.2.7 Mg2+ required Caenorhabditis elegans
7.3.2.7 Sb3+ activates the ATPase activity of the ASNA-1 protein 4.6fold, Km is 0.04 mM at ATP saturation Caenorhabditis elegans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7.3.2.7 ATP + H2O + arsenite/in Caenorhabditis elegans ASNA-1 , encoding a functional ArsA ATPase, is critical for As(III) and Sb(III) tolerance in the intact organism, the enzyme does not provide resistance to other metals, e.g. Cu2+ or Pb2+, overview ADP + phosphate + arsenite/out
-
?

Organism

EC Number Organism UniProt Comment Textmining
7.3.2.7 Caenorhabditis elegans P30632 gene asna-1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
7.3.2.7 recombinant maltose-binding-protein fusion protein from Escherichia coli by amylose affinity chromatography Caenorhabditis elegans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.3.2.7 28.87
-
purified recombinant MBP-fusion enzyme stimulated by 0.5 mM Sb3+ Caenorhabditis elegans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.3.2.7 ATP + H2O + arsenite/in
-
Caenorhabditis elegans ADP + phosphate + arsenite/out
-
?
7.3.2.7 ATP + H2O + arsenite/in ASNA-1 , encoding a functional ArsA ATPase, is critical for As(III) and Sb(III) tolerance in the intact organism, the enzyme does not provide resistance to other metals, e.g. Cu2+ or Pb2+, overview Caenorhabditis elegans ADP + phosphate + arsenite/out
-
?

Synonyms

EC Number Synonyms Comment Organism
7.3.2.7 ArsA ATPase
-
Caenorhabditis elegans
7.3.2.7 ASNA-1
-
Caenorhabditis elegans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
7.3.2.7 37
-
assay at Caenorhabditis elegans

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
7.3.2.7 25 37
-
Caenorhabditis elegans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
7.3.2.7 7.4
-
assay at Caenorhabditis elegans