EC Number | Cloned (Comment) | Organism |
---|---|---|
7.3.2.7 | expression of the enzyme as maltose-binding-protein fusion protein in Escherichia coli | Caenorhabditis elegans |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
7.3.2.7 | additional information | asna-1-mutant nematodes are more sensitive to As(III) and Sb(III) toxicity than are wild-type animals | Caenorhabditis elegans |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
7.3.2.7 | 0.00004 | - |
Sb3+ | - |
Caenorhabditis elegans | |
7.3.2.7 | 0.021 | - |
ATP | pH 7.4, 37°C, wild-type enzyme in absence of Sb3+ | Caenorhabditis elegans | |
7.3.2.7 | 0.026 | - |
ATP | pH 7.4, 37°C, wild-type enzyme in presence of Sb3+ | Caenorhabditis elegans |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
7.3.2.7 | As3+ | activates the ATPase activity of the ASNA-1 protein 2.0fold, Km is 3.54 mM at ATP saturation | Caenorhabditis elegans | |
7.3.2.7 | Mg2+ | required | Caenorhabditis elegans | |
7.3.2.7 | Sb3+ | activates the ATPase activity of the ASNA-1 protein 4.6fold, Km is 0.04 mM at ATP saturation | Caenorhabditis elegans |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
7.3.2.7 | ATP + H2O + arsenite/in | Caenorhabditis elegans | ASNA-1 , encoding a functional ArsA ATPase, is critical for As(III) and Sb(III) tolerance in the intact organism, the enzyme does not provide resistance to other metals, e.g. Cu2+ or Pb2+, overview | ADP + phosphate + arsenite/out | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
7.3.2.7 | Caenorhabditis elegans | P30632 | gene asna-1 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
7.3.2.7 | recombinant maltose-binding-protein fusion protein from Escherichia coli by amylose affinity chromatography | Caenorhabditis elegans |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
7.3.2.7 | 28.87 | - |
purified recombinant MBP-fusion enzyme stimulated by 0.5 mM Sb3+ | Caenorhabditis elegans |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
7.3.2.7 | ATP + H2O + arsenite/in | - |
Caenorhabditis elegans | ADP + phosphate + arsenite/out | - |
? | |
7.3.2.7 | ATP + H2O + arsenite/in | ASNA-1 , encoding a functional ArsA ATPase, is critical for As(III) and Sb(III) tolerance in the intact organism, the enzyme does not provide resistance to other metals, e.g. Cu2+ or Pb2+, overview | Caenorhabditis elegans | ADP + phosphate + arsenite/out | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
7.3.2.7 | ArsA ATPase | - |
Caenorhabditis elegans |
7.3.2.7 | ASNA-1 | - |
Caenorhabditis elegans |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
7.3.2.7 | 37 | - |
assay at | Caenorhabditis elegans |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
7.3.2.7 | 25 | 37 | - |
Caenorhabditis elegans |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
7.3.2.7 | 7.4 | - |
assay at | Caenorhabditis elegans |