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Literature summary extracted from

  • Lee, M.H.; Yang, S.J.; Kim, J.W.; Lee, H.S.; Kim, J.W.; Park, K.H.
    Characterization of a thermostable cyclodextrin glucanotransferase from Pyrococcus furiosus DSM3638 (2007), Extremophiles, 11, 537-541.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.19 gene pfcgt, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression in Escherichia coli Pyrococcus furiosus

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.19 Pyrococcus furiosus Q3HUR2 DSM3638, gene pfcgt
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.19 additional information reversible cyclization reaction with alpha-1,4-glucans, e.g. starch, the major final product of PFCGT cyclization is beta-cyclodextrin, and thus the enzyme is a beta-CGTase Pyrococcus furiosus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.19 PFCGT
-
 Pyrococcus furiosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.19 95
-
 purified enzyme Pyrococcus furiosus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.19 60
-
 purified enzyme, 20 min during the purification process, 1.5fold increased enzymatic activity Pyrococcus furiosus
2.4.1.19 85
-
 purified enzyme, half-life is 142 min Pyrococcus furiosus
2.4.1.19 90
-
 purified enzyme, half-life is 85 min Pyrococcus furiosus
2.4.1.19 95
-
 purified enzyme, half-life is 46 min Pyrococcus furiosus
2.4.1.19 100
-
 purified enzyme, 20 min, 97% remaining activity Pyrococcus furiosus
2.4.1.19 105
-
 purified enzyme, 20 min, 60% remaining activity Pyrococcus furiosus
2.4.1.19 110
-
 purified enzyme, 20 min, 45% remaining activity Pyrococcus furiosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.19 5
-
 purified enzyme Pyrococcus furiosus