BRENDA - Enzyme Database show

Biochemical studies on inositol. V. Purification and properties of the enzyme that cleaves inositol to D-glucuronic acid

Charalampous, F.C.; J. Biol. Chem. 234, 220-227 (1959)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
1.13.99.1
cysteine
best activation system: 1 mM Fe(II) and 2 mM cysteine
Rattus norvegicus
1.13.99.1
quinolinate
1 mM Fe(II) + 4 mM quinolinate activates to 70% of the Fe(II)/cysteine system, Fe(II) alone causes very little activation, quinolinate gives considerable activation in absence of Fe(II), activation by Fe(II) and quinolinate is very temperature dependent
Rattus norvegicus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.13.99.1
2-Thenoyltrifluoroacetone
slight
Rattus norvegicus
1.13.99.1
8-hydroxyquinoline
-
Rattus norvegicus
1.13.99.1
ADP
slight
Rattus norvegicus
1.13.99.1
Ag+
-
Rattus norvegicus
1.13.99.1
AMP
-
Rattus norvegicus
1.13.99.1
arsenite
-
Rattus norvegicus
1.13.99.1
ATP
-
Rattus norvegicus
1.13.99.1
azide
-
Rattus norvegicus
1.13.99.1
CTP
-
Rattus norvegicus
1.13.99.1
Cu2+
-
Rattus norvegicus
1.13.99.1
cyanide
-
Rattus norvegicus
1.13.99.1
FAD
-
Rattus norvegicus
1.13.99.1
Furoylthiofluoroacetone
slight
Rattus norvegicus
1.13.99.1
GTP
-
Rattus norvegicus
1.13.99.1
Hg2+
-
Rattus norvegicus
1.13.99.1
hydroxylamine
-
Rattus norvegicus
1.13.99.1
iodoacetate
-
Rattus norvegicus
1.13.99.1
menadione
-
Rattus norvegicus
1.13.99.1
NAD+
-
Rattus norvegicus
1.13.99.1
NADP+
-
Rattus norvegicus
1.13.99.1
o-phenanthroline
slight
Rattus norvegicus
1.13.99.1
p-chloromercuribenzoate
-
Rattus norvegicus
1.13.99.1
Phenylmercuric nitrate
-
Rattus norvegicus
1.13.99.1
Quinacrine hydrochloride
slight
Rattus norvegicus
1.13.99.1
riboflavin phosphate
-
Rattus norvegicus
1.13.99.1
tetrahydrofolic acid
-
Rattus norvegicus
1.13.99.1
Uridine diphosphoglucose
-
Rattus norvegicus
1.13.99.1
UTP
-
Rattus norvegicus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.13.99.1
22.1
-
Inositol
-
Rattus norvegicus
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.13.99.1
Fe2+
1 mM Fe(II) + 4 mM quinolinate activate to 70% of the Fe(II)/cysteine system, Fe(II) alone causes very little activation, quinolinate gives considerable activation in absence of Fe(II), activation by Fe(II) and quinolinate is very temperature dependent; 1 mM Fe(II) and 2 mM cysteine; best activation system
Rattus norvegicus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.13.99.1
Rattus norvegicus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.13.99.1
-
Rattus norvegicus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.13.99.1
kidney
-
Rattus norvegicus
-
Storage Stability
EC Number
Storage Stability
Organism
1.13.99.1
-20°C, extensive loss of activity after 1 or 2 days
Rattus norvegicus
1.13.99.1
0°C, 12 h, extensive loss of activity
Rattus norvegicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.13.99.1
myo-inositol + O2
highly specific for myo-inositol
6866
Rattus norvegicus
D-glucuronate + H2O
-
-
-
-
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.13.99.1
35
-
assay at
Rattus norvegicus
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.13.99.1
7.22
-
Inositol
-
Rattus norvegicus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.13.99.1
6.8
7.2
-
Rattus norvegicus
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.13.99.1
6.5
7.4
sharp decrease of activity below pH 6.5 and above pH 7.4
Rattus norvegicus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.13.99.1
flavin
5. 6 mMol per mol of enzyme
Rattus norvegicus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
1.13.99.1
cysteine
best activation system: 1 mM Fe(II) and 2 mM cysteine
Rattus norvegicus
1.13.99.1
quinolinate
1 mM Fe(II) + 4 mM quinolinate activates to 70% of the Fe(II)/cysteine system, Fe(II) alone causes very little activation, quinolinate gives considerable activation in absence of Fe(II), activation by Fe(II) and quinolinate is very temperature dependent
Rattus norvegicus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.13.99.1
flavin
5. 6 mMol per mol of enzyme
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.13.99.1
2-Thenoyltrifluoroacetone
slight
Rattus norvegicus
1.13.99.1
8-hydroxyquinoline
-
Rattus norvegicus
1.13.99.1
ADP
slight
Rattus norvegicus
1.13.99.1
Ag+
-
Rattus norvegicus
1.13.99.1
AMP
-
Rattus norvegicus
1.13.99.1
arsenite
-
Rattus norvegicus
1.13.99.1
ATP
-
Rattus norvegicus
1.13.99.1
azide
-
Rattus norvegicus
1.13.99.1
CTP
-
Rattus norvegicus
1.13.99.1
Cu2+
-
Rattus norvegicus
1.13.99.1
cyanide
-
Rattus norvegicus
1.13.99.1
FAD
-
Rattus norvegicus
1.13.99.1
Furoylthiofluoroacetone
slight
Rattus norvegicus
1.13.99.1
GTP
-
Rattus norvegicus
1.13.99.1
Hg2+
-
Rattus norvegicus
1.13.99.1
hydroxylamine
-
Rattus norvegicus
1.13.99.1
iodoacetate
-
Rattus norvegicus
1.13.99.1
menadione
-
Rattus norvegicus
1.13.99.1
NAD+
-
Rattus norvegicus
1.13.99.1
NADP+
-
Rattus norvegicus
1.13.99.1
o-phenanthroline
slight
Rattus norvegicus
1.13.99.1
p-chloromercuribenzoate
-
Rattus norvegicus
1.13.99.1
Phenylmercuric nitrate
-
Rattus norvegicus
1.13.99.1
Quinacrine hydrochloride
slight
Rattus norvegicus
1.13.99.1
riboflavin phosphate
-
Rattus norvegicus
1.13.99.1
tetrahydrofolic acid
-
Rattus norvegicus
1.13.99.1
Uridine diphosphoglucose
-
Rattus norvegicus
1.13.99.1
UTP
-
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.13.99.1
22.1
-
Inositol
-
Rattus norvegicus
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.13.99.1
Fe2+
1 mM Fe(II) + 4 mM quinolinate activate to 70% of the Fe(II)/cysteine system, Fe(II) alone causes very little activation, quinolinate gives considerable activation in absence of Fe(II), activation by Fe(II) and quinolinate is very temperature dependent; 1 mM Fe(II) and 2 mM cysteine; best activation system
Rattus norvegicus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.13.99.1
-
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.13.99.1
kidney
-
Rattus norvegicus
-
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
1.13.99.1
-20°C, extensive loss of activity after 1 or 2 days
Rattus norvegicus
1.13.99.1
0°C, 12 h, extensive loss of activity
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.13.99.1
myo-inositol + O2
highly specific for myo-inositol
6866
Rattus norvegicus
D-glucuronate + H2O
-
-
-
-
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.13.99.1
35
-
assay at
Rattus norvegicus
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.13.99.1
7.22
-
Inositol
-
Rattus norvegicus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.13.99.1
6.8
7.2
-
Rattus norvegicus
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.13.99.1
6.5
7.4
sharp decrease of activity below pH 6.5 and above pH 7.4
Rattus norvegicus