BRENDA - Enzyme Database

Functional evaluation of the genes involved in malonate decarboxylation by Acinetobacter calcoaceticus

Koo, J.H.; Kim, Y.S.; Eur. J. Biochem. 266, 683-690 (1999)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.3.1.187
expression in Escherichia coli
Acinetobacter calcoaceticus
4.1.1.87
expression in Escherichia coli
Acinetobacter calcoaceticus
4.1.1.88
expression in Escherichia coli
Acinetobacter calcoaceticus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.187
0.01
-
methylmalonyl-CoA
-
Acinetobacter calcoaceticus
2.3.1.187
0.05
-
malonyl-CoA
-
Acinetobacter calcoaceticus
4.1.1.87
1.2
-
malonyl-CoA
-
Acinetobacter calcoaceticus
4.1.1.88
0.11
-
malonate
apo-enzyme in presence of acetyl-CoA
Acinetobacter calcoaceticus
4.1.1.88
0.16
-
malonate
subunit beta in presence of acetyl-CoA; subunits beta/gamma in presence of acetyl-CoA
Acinetobacter calcoaceticus
4.1.1.88
3.23
-
malonate
apo-enzyme in presence of acetyl-CoA
Acinetobacter calcoaceticus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.1.1.87
33000
-
2 * 33000, SDS-PAGE, isolated beta subunit of malonate decarboxylase complex
Acinetobacter calcoaceticus
4.1.1.87
66000
-
PAGE
Acinetobacter calcoaceticus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.3.1.187
Acinetobacter calcoaceticus
-
MdcH
-
2.3.1.187
Acinetobacter calcoaceticus MdcH
-
MdcH
-
4.1.1.87
Acinetobacter calcoaceticus
O54416
beta subunit of malonate decarboxylase complex
-
4.1.1.88
Acinetobacter calcoaceticus
-
-
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
2.3.1.187
18.3
-
-
Acinetobacter calcoaceticus
4.1.1.87
0.19
-
isolated beta subunit of malonate decarboxylase complex
Acinetobacter calcoaceticus
4.1.1.88
additional information
-
the alpha subunit shows CoA-transferase activity with specific activity of 39.03 U/mg
Acinetobacter calcoaceticus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.187
malonyl-CoA + dephospho-CoA
-
686494
Acinetobacter calcoaceticus
CoA + malonyl-dephospho-CoA
-
-
-
?
2.3.1.187
malonyl-CoA + dephospho-CoA
-
686494
Acinetobacter calcoaceticus MdcH
CoA + malonyl-dephospho-CoA
-
-
-
?
2.3.1.187
methylmalonyl-CoA + dephospho-CoA
-
686494
Acinetobacter calcoaceticus
CoA + methylmalonyl-dephospho-CoA
-
-
-
?
2.3.1.187
methylmalonyl-CoA + dephospho-CoA
-
686494
Acinetobacter calcoaceticus MdcH
CoA + methylmalonyl-dephospho-CoA
-
-
-
?
4.1.1.87
malonyl-CoA + H+
-
686494
Acinetobacter calcoaceticus
acetyl-CoA + CO2
-
-
-
?
4.1.1.88
malonate + H+
-
686494
Acinetobacter calcoaceticus
acetate + CO2
-
-
-
?
4.1.1.88
malonate + H+
-
686494
Acinetobacter calcoaceticus MdcH
acetate + CO2
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
4.1.1.87
dimer
2 * 33000, SDS-PAGE, isolated beta subunit of malonate decarboxylase complex
Acinetobacter calcoaceticus
4.1.1.87
More
mdcD gene product is the beta subunit of malonate decarboxylase carrying the malonyl-CoA decarboxylase activity
Acinetobacter calcoaceticus
4.1.1.88
More
enzyme complex is formed by subunits alpha, beta, gamma, delta. The mdcA gene product, the alpha subunit, is malonate/acetyl-CoA transferase and the mdcD gene product, the beta subunit, is malonyl-CoA decarboxylase. The mdcE gene product, the gamma subunit, may play a role in subunit interaction to form a stable complex or as a codecarboxylase. The mdcC gene product, the delta subunit, is an acyl-carrier protein
Acinetobacter calcoaceticus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
4.1.1.88
additional information
the prosthetic group of the acyl carrier protein is carboxymethylated 2'-(5''-phosphoribosyl)-3'-dephospho-CoA
Acinetobacter calcoaceticus
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.3.1.187
expression in Escherichia coli
Acinetobacter calcoaceticus
4.1.1.87
expression in Escherichia coli
Acinetobacter calcoaceticus
4.1.1.88
expression in Escherichia coli
Acinetobacter calcoaceticus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
4.1.1.88
additional information
the prosthetic group of the acyl carrier protein is carboxymethylated 2'-(5''-phosphoribosyl)-3'-dephospho-CoA
Acinetobacter calcoaceticus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.187
0.01
-
methylmalonyl-CoA
-
Acinetobacter calcoaceticus
2.3.1.187
0.05
-
malonyl-CoA
-
Acinetobacter calcoaceticus
4.1.1.87
1.2
-
malonyl-CoA
-
Acinetobacter calcoaceticus
4.1.1.88
0.11
-
malonate
apo-enzyme in presence of acetyl-CoA
Acinetobacter calcoaceticus
4.1.1.88
0.16
-
malonate
subunit beta in presence of acetyl-CoA; subunits beta/gamma in presence of acetyl-CoA
Acinetobacter calcoaceticus
4.1.1.88
3.23
-
malonate
apo-enzyme in presence of acetyl-CoA
Acinetobacter calcoaceticus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.1.1.87
33000
-
2 * 33000, SDS-PAGE, isolated beta subunit of malonate decarboxylase complex
Acinetobacter calcoaceticus
4.1.1.87
66000
-
PAGE
Acinetobacter calcoaceticus
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
2.3.1.187
18.3
-
-
Acinetobacter calcoaceticus
4.1.1.87
0.19
-
isolated beta subunit of malonate decarboxylase complex
Acinetobacter calcoaceticus
4.1.1.88
additional information
-
the alpha subunit shows CoA-transferase activity with specific activity of 39.03 U/mg
Acinetobacter calcoaceticus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.187
malonyl-CoA + dephospho-CoA
-
686494
Acinetobacter calcoaceticus
CoA + malonyl-dephospho-CoA
-
-
-
?
2.3.1.187
malonyl-CoA + dephospho-CoA
-
686494
Acinetobacter calcoaceticus MdcH
CoA + malonyl-dephospho-CoA
-
-
-
?
2.3.1.187
methylmalonyl-CoA + dephospho-CoA
-
686494
Acinetobacter calcoaceticus
CoA + methylmalonyl-dephospho-CoA
-
-
-
?
2.3.1.187
methylmalonyl-CoA + dephospho-CoA
-
686494
Acinetobacter calcoaceticus MdcH
CoA + methylmalonyl-dephospho-CoA
-
-
-
?
4.1.1.87
malonyl-CoA + H+
-
686494
Acinetobacter calcoaceticus
acetyl-CoA + CO2
-
-
-
?
4.1.1.88
malonate + H+
-
686494
Acinetobacter calcoaceticus
acetate + CO2
-
-
-
?
4.1.1.88
malonate + H+
-
686494
Acinetobacter calcoaceticus MdcH
acetate + CO2
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.1.1.87
dimer
2 * 33000, SDS-PAGE, isolated beta subunit of malonate decarboxylase complex
Acinetobacter calcoaceticus
4.1.1.87
More
mdcD gene product is the beta subunit of malonate decarboxylase carrying the malonyl-CoA decarboxylase activity
Acinetobacter calcoaceticus
4.1.1.88
More
enzyme complex is formed by subunits alpha, beta, gamma, delta. The mdcA gene product, the alpha subunit, is malonate/acetyl-CoA transferase and the mdcD gene product, the beta subunit, is malonyl-CoA decarboxylase. The mdcE gene product, the gamma subunit, may play a role in subunit interaction to form a stable complex or as a codecarboxylase. The mdcC gene product, the delta subunit, is an acyl-carrier protein
Acinetobacter calcoaceticus