BRENDA - Enzyme Database

Malonate decarboxylase of Malonomonas rubra, a novel type of biotin-containing acetyl enzyme

Hilbi, H.; Dehning, I.; Schink, B.; Dimroth, P.; Eur. J. Biochem. 207, 117-123 (1992)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
7.2.4.4
hydroxylamine
complete inhibition
Malonomonas rubra
7.2.4.4
thiocyanate
complete inhibition
Malonomonas rubra
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
7.2.4.4
Malonomonas rubra
-
-
-
Reaction
EC Number
Reaction
Commentary
Organism
7.2.4.4
malonate + H+ + Na+[side 1] = acetate + CO2 + Na+[side 2]
reaction does not involve intermediate formation of malonyl-CoA but proceeds directly with free malonate. Catalytic mechanism involves exchange of the enzyme-bound acetyl residues by malonyl residues and subsequent decarboxylation releasing CO2 and regenerating the acetyl-enzyme. Biotin is involved in catalysis
Malonomonas rubra
Renatured (Commentary)
EC Number
Commentary
Organism
7.2.4.4
enzyme inhibited by thiocyanate or hydroxylamine, 50-65% of the original decarboxylase activity is restored by incubation of the extract with ATP in the presence of acetate, and the extent of reactivation increases after incubation with dithioerythritol. Reactivation is also obtained by chemical acetylation with acetic anhydride
Malonomonas rubra
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
7.2.4.4
2.67
-
pH 7.5, 30░C
Malonomonas rubra
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
7.2.4.4
malonate + H+
-
686488
Malonomonas rubra
acetate + CO2
-
-
-
?
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
7.2.4.4
hydroxylamine
complete inhibition
Malonomonas rubra
7.2.4.4
thiocyanate
complete inhibition
Malonomonas rubra
Renatured (Commentary) (protein specific)
EC Number
Commentary
Organism
7.2.4.4
enzyme inhibited by thiocyanate or hydroxylamine, 50-65% of the original decarboxylase activity is restored by incubation of the extract with ATP in the presence of acetate, and the extent of reactivation increases after incubation with dithioerythritol. Reactivation is also obtained by chemical acetylation with acetic anhydride
Malonomonas rubra
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
7.2.4.4
2.67
-
pH 7.5, 30░C
Malonomonas rubra
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
7.2.4.4
malonate + H+
-
686488
Malonomonas rubra
acetate + CO2
-
-
-
?