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Literature summary extracted from
- Biochemical characterization of the coexisting tyrosinase and laccase in the soil bacterium Pseudomonas putida F6 (2007), Enzyme Microb. Technol., 40, 1435-1441.
No PubMed abstract available
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 1.14.18.1 | monophenolase and diphenolase activities of tyrosinase are relatively stable across a broad range of pH values with maximum stability at pH 5.0 and 4.0. | Pseudomonas putida |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.10.3.2 | 0.11 | - |
syringaldazine | enzyme has a higher affinity for syringaldazine than for L-dopa | Pseudomonas putida |  |
| 1.10.3.2 | 0.84 | - |
L-Dopa | enzyme has a higher affinity for syringaldazine than for L-dopa | Pseudomonas putida | |
| 1.14.18.1 | 0.23 | - |
L-tyrosine | - |
Pseudomonas putida | |
| 1.14.18.1 | 0.33 | - |
L-Dopa | - |
Pseudomonas putida | |
| 1.14.18.1 | 1.12 | - |
alpha-methyl-DL-tyrosine | - |
Pseudomonas putida | |
| 1.14.18.1 | 3.07 | - |
alpha-methyl-L-tyrosine | - |
Pseudomonas putida | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.10.3.2 | cytoplasm | - |
Pseudomonas putida | 5737 | - |
| 1.14.18.1 | cytoplasm | - |
Pseudomonas putida | 5737 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.10.3.2 | copper | - |
Pseudomonas putida | |
| 1.14.18.1 | copper | - |
Pseudomonas putida | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.10.3.2 | 59000 | - |
indicated by gel filtration chromatography | Pseudomonas putida |
| 1.14.18.1 | 36000 | - |
determination by gel filtration chromatography | Pseudomonas putida |
| 1.14.18.1 | 39000 | - |
determination by SDS-PAGE | Pseudomonas putida |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.10.3.2 | Pseudomonas putida | - |
soil bacterium. The coexistence of this tyrosinase with a laccase in Pseudomonas putida F6 is reported and both activities characterised | - |
| 1.10.3.2 | Pseudomonas putida F6 | - |
soil bacterium. The coexistence of this tyrosinase with a laccase in Pseudomonas putida F6 is reported and both activities characterised | - |
| 1.14.18.1 | Pseudomonas putida | - |
soil bacterium. The coexistence of this tyrosinase with a laccase in Pseudomonas putida F6 is reported and both activities characterised | - |
| 1.14.18.1 | Pseudomonas putida F6 | - |
soil bacterium. The coexistence of this tyrosinase with a laccase in Pseudomonas putida F6 is reported and both activities characterised | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.10.3.2 | combination of anion exchange chromatography, gel filtration and gel slicing | Pseudomonas putida |
| 1.14.18.1 | combination of anion exchange chromatography, gel filtration and gel slicing | Pseudomonas putida |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.10.3.2 | 0.011 | - |
CE, purification of Pseudomonas putida F6 laccase-type activity | Pseudomonas putida |
| 1.10.3.2 | 0.061 | - |
DEAE, purification of Pseudomonas putida F6 laccase-type activity | Pseudomonas putida |
| 1.10.3.2 | 0.573 | - |
Resource Q, purification of Pseudomonas putida F6 laccase-type activity | Pseudomonas putida |
| 1.10.3.2 | 4.57 | - |
gel filtration, purification of Pseudomonas putida F6 laccase-type activity | Pseudomonas putida |
| 1.14.18.1 | 0.02912 | - |
CE, purification, monophenolase activity | Pseudomonas putida |
| 1.14.18.1 | 0.05512 | - |
CE, purification, diphenolase activity | Pseudomonas putida |
| 1.14.18.1 | 0.375 | - |
DEAE-sepharose, purification of Pseudomonas putida F6 tyrosinase, monophenolase activity | Pseudomonas putida |
| 1.14.18.1 | 0.8125 | - |
DEAE-sepharose, purification, diphenolase activity | Pseudomonas putida |
| 1.14.18.1 | 2.143 | - |
mono Q, specific activity (units/mg) purification, monophenolase activity | Pseudomonas putida |
| 1.14.18.1 | 2.964 | - |
gel filtration, purification, monophenolase activity | Pseudomonas putida |
| 1.14.18.1 | 3.058 | - |
gel slice, purification, monophenolase activity | Pseudomonas putida |
| 1.14.18.1 | 4.725 | - |
mono Q, purification, diphenolase activity | Pseudomonas putida |
| 1.14.18.1 | 4.818 | - |
gel filtration, purification, diphenolase activity | Pseudomonas putida |
| 1.14.18.1 | 5.463 | - |
gel slice, purification, diphenolase activity | Pseudomonas putida |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.10.3.2 | L-DOPA + O2 | - |
Pseudomonas putida | ? + H2O | - |
? | |
| 1.10.3.2 | L-DOPA + O2 | - |
Pseudomonas putida F6 | ? + H2O | - |
? | |
| 1.10.3.2 | syringaldazine + O2 | - |
Pseudomonas putida | ? | - |
? | |
| 1.10.3.2 | syringaldazine + O2 | - |
Pseudomonas putida F6 | ? | - |
? | |
| 1.14.18.1 | alpha-methyl-DL-tyrosine + O2 + AH2 | - |
Pseudomonas putida | N-methyl-DL-dopa + H2O + A | - |
? | |
| 1.14.18.1 | alpha-methyl-DL-tyrosine + O2 + AH2 | - |
Pseudomonas putida F6 | N-methyl-DL-dopa + H2O + A | - |
? | |
| 1.14.18.1 | alpha-methyl-L-tyrosine + O2 + AH2 | - |
Pseudomonas putida | N-methyl-L-dopa + H2O + A | - |
? | |
| 1.14.18.1 | L-dopa + 1/2 O2 | 1.5fold higher affinity for L-tyrosine compared to L-dopa | Pseudomonas putida | L-dopaquinone + H2O | - |
? | |
| 1.14.18.1 | L-dopa + 1/2 O2 | 1.5fold higher affinity for L-tyrosine compared to L-dopa | Pseudomonas putida F6 | L-dopaquinone + H2O | - |
? | |
| 1.14.18.1 | L-tyrosine + O2 + AH2 | 1.5fold higher affinity for L-tyrosine compared to L-dopa | Pseudomonas putida | L-dopa + H2O + A | - |
? | |
| 1.14.18.1 | L-tyrosine + O2 + AH2 | 1.5fold higher affinity for L-tyrosine compared to L-dopa | Pseudomonas putida F6 | L-dopa + H2O + A | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.14.18.1 | monomer | 1 * 36000-39000 SDS-PAGE | Pseudomonas putida |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.10.3.2 | laccase | - |
Pseudomonas putida |
| 1.14.18.1 | tyrosinase | - |
Pseudomonas putida |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.10.3.2 | 30 | - |
assay at | Pseudomonas putida |
| 1.14.18.1 | 30 | - |
assay at | Pseudomonas putida |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.10.3.2 | 30 | - |
stable over a broad range of temperatures and are most stable at 30°C | Pseudomonas putida |
| 1.14.18.1 | 30 | - |
stable over a broad range of temperatures and are most stable at 30°C | Pseudomonas putida |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.10.3.2 | 7 | - |
assay at, most stable at pH 7.0 | Pseudomonas putida |
| 1.14.18.1 | 7 | - |
assay at | Pseudomonas putida |
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