Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Ferrara, M.A.; Severino, N.M.; Mansure, J.J.; Martins, A.S.; Oliveira, E.M.; Siani, A.C.; Pereira, N.; Torres, F.A.; Bon, E.P.
    Asparaginase production by a recombinant Pichia pastoris strain harbouring Saccharomyces cerevisiae ASP3 gene (2006), Enzyme Microb. Technol., 39, 1457-1463.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.1 expressed in Pichia pastoris under the control of the AOX1 gene promoter. High cell density cultures performed with Pichia pastoris harbouring the ASP3 gene using a 2 l instrumented bioreactor, where biomass concentration reached 107 g/l, results in a dramatic increase in volumetric yield (85600 U/l) and global volumetric productivity (1083 U/l) Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.1.1 periplasmic space
-
 Saccharomyces cerevisiae 42597
-

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.1 Saccharomyces cerevisiae
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.1 L-asparagine + H2O
-
 Saccharomyces cerevisiae L-aspartate + NH3
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.1.1 asparaginase II
-
 Saccharomyces cerevisiae