BRENDA - Enzyme Database

Involvement of deoxycytidylate deaminase in the response to Sn1-type methylation DNA damage in budding yeast

Liskay, R.M.; Wheeler, L.J.; Mathews, C.K.; Erdeniz, N.; Curr. Biol. 17, R755-R757 (2007)

Data extracted from this reference:

Engineering
EC Number
Amino acid exchange
Commentary
Organism
3.5.4.12
additional information
construction of a dcd1DELTA enzyme-deficient strain, the deletion strain and the dcd1-S178F mutant strain are crossed with a rad52DELTA mgt1DELTA strain and haploid, double and triple mutant strains are generated, rad52DELTA mgt1DELTA dcd1-S178F and rad52DELTA mgt1DELTA dcd1DELTA strains show an increased level of MNNG resistance, while the only dcd1-deficient strain does not, overview, DCD1 deficiency in yeast results in elevation of dCTP pools and a slight decrease in dTTP pools and reduced the frequency of spontaneous and MNNGinduced G/C to A/T mutations, phenotypes, overview
Saccharomyces cerevisiae
3.5.4.12
S178F
site-directed mutagenesis, the mutation is responsible for MNNG resistance in the drm1-1 strain,
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.4.12
dCMP + H2O
Saccharomyces cerevisiae
-
dUMP + NH3
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.4.12
Saccharomyces cerevisiae
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.4.12
dCMP + H2O
-
686248
Saccharomyces cerevisiae
dUMP + NH3
-
-
-
?
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
3.5.4.12
additional information
construction of a dcd1DELTA enzyme-deficient strain, the deletion strain and the dcd1-S178F mutant strain are crossed with a rad52DELTA mgt1DELTA strain and haploid, double and triple mutant strains are generated, rad52DELTA mgt1DELTA dcd1-S178F and rad52DELTA mgt1DELTA dcd1DELTA strains show an increased level of MNNG resistance, while the only dcd1-deficient strain does not, overview, DCD1 deficiency in yeast results in elevation of dCTP pools and a slight decrease in dTTP pools and reduced the frequency of spontaneous and MNNGinduced G/C to A/T mutations, phenotypes, overview
Saccharomyces cerevisiae
3.5.4.12
S178F
site-directed mutagenesis, the mutation is responsible for MNNG resistance in the drm1-1 strain,
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.4.12
dCMP + H2O
Saccharomyces cerevisiae
-
dUMP + NH3
-
-
?
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.4.12
dCMP + H2O
-
686248
Saccharomyces cerevisiae
dUMP + NH3
-
-
-
?