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Literature summary extracted from

  • Verma, N.; Kumar, K.; Kaur, G.; Anand, S.
    L-asparaginase: a promising chemotherapeutic agent (2007), Crit. Rev. Biotechnol., 27, 45-62.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.1.1 Lipid activates Tetrahymena pyriformis

Application

EC Number Application Comment Organism
3.5.1.1 analysis direct measurement of L-asparagine in human plasma samples through the use of Escherichia coli Lasparaginase in the soluble form is a major clinical application of this system Escherichia coli

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.1
-
 Lupinus angustifolius
3.5.1.1
-
 Lupinus arboreus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.1 Fe2+
-
 Rhodotorula mucilaginosa
3.5.1.1 Pb2+
-
 Rhodotorula mucilaginosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.1 0.7
-
 L-asparagine
-
 Mycolicibacterium phlei
3.5.1.1 2.8
-
 L-asparagine
-
 Erwinia sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.1.1 extracellular
-
 Cyberlindnera jadinii
-
-
3.5.1.1 extracellular
-
 Rhodotorula toruloides
-
-
3.5.1.1 intracellular
-
 Enterobacter cloacae 5622
-
3.5.1.1 microsome
-
 Tetrahymena pyriformis
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.1 Mg2+ activates Rhodotorula mucilaginosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.1 33000
-
 x * 33000, SDS-PAGE Thermus thermophilus
3.5.1.1 120000
-
-
 Sphagnum fallax

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.1 additional information Escherichia coli L-asparaginase from Escherichia coli is more immuno-depressive and immunotoxic than that from Erwinia carotovora ?
-
 ?
3.5.1.1 additional information Pectobacterium carotovorum L-asparaginase from Escherichia coli is more immuno-depressive and immunotoxic than that from Erwinia carotovora ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.1 Aliivibrio fischeri
-
-
-
3.5.1.1 Aspergillus niger
-
-
-
3.5.1.1 Aspergillus tamarii
-
-
-
3.5.1.1 Aspergillus terreus
-
-
-
3.5.1.1 Cyberlindnera jadinii
-
-
-
3.5.1.1 Enterobacter cloacae
-
-
-
3.5.1.1 Erwinia aroidea
-
 NRR LB-138
-
3.5.1.1 Erwinia aroidea NRR LB-138
-
 NRR LB-138
-
3.5.1.1 Erwinia sp.
-
-
-
3.5.1.1 Escherichia coli
-
 strain W and strain K-12
-
3.5.1.1 Lupinus angustifolius
-
-
-
3.5.1.1 Lupinus arboreus
-
-
-
3.5.1.1 Mycolicibacterium phlei
-
-
-
3.5.1.1 Nocardia asteroides
-
-
-
3.5.1.1 Pectobacterium carotovorum
-
-
-
3.5.1.1 Photobacterium leiognathi
-
-
-
3.5.1.1 Photobacterium phosphoreum
-
-
-
3.5.1.1 Pseudomonas aeruginosa
-
-
-
3.5.1.1 Pseudomonas aeruginosa 50071
-
-
-
3.5.1.1 Pseudomonas fluorescens
-
-
-
3.5.1.1 Pseudomonas putida
-
-
-
3.5.1.1 Rhodotorula mucilaginosa
-
-
-
3.5.1.1 Rhodotorula toruloides
-
-
-
3.5.1.1 Saccharomyces cerevisiae
-
-
-
3.5.1.1 Saccharomyces cerevisiae P38986
-
-
3.5.1.1 Serratia marcescens
-
-
-
3.5.1.1 Sphagnum fallax
-
-
-
3.5.1.1 Staphylococcus sp.
-
-
-
3.5.1.1 Tetrahymena pyriformis
-
-
-
3.5.1.1 Thermus thermophilus
-
-
-
3.5.1.1 Vibrio harveyi
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.1
-
 Cyberlindnera jadinii
3.5.1.1
-
 Thermus thermophilus
3.5.1.1
-
 Pseudomonas aeruginosa
3.5.1.1
-
 Mycolicibacterium phlei
3.5.1.1
-
 Sphagnum fallax

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.1 culture condition carbon sources such as sucrose, maltose, galactose, lactose, mannitol and mannose inhibit enzyme production. Exogenous cAMP in presence of carbon sources stimulates L-asparaginase enzyme production Staphylococcus sp.
-
3.5.1.1 culture condition high L-asparaginase activity is found in cells cultured on L-fructose, D-galactose, sucrose or maltose, and in cells cultured on L-asparagine as the sole nitrogen source Enterobacter cloacae
-
3.5.1.1 culture condition L-asparaginase-I is constitutive Saccharomyces cerevisiae
-
3.5.1.1 culture condition L-asparaginase-II is secreted in response to N starvation Saccharomyces cerevisiae
-
3.5.1.1 culture condition sabourand dextrose broth yields maximum growth and maximum L-asparaginase production Nocardia asteroides
-
3.5.1.1 flower bud
-
 Lupinus arboreus
-
3.5.1.1 leaf
-
 Lupinus arboreus
-
3.5.1.1 additional information a pH of 7.9, casein hydrolysate (3.11%) and corn-steep liquor (3.68%) are the most significant factors improving the enzyme production process Pseudomonas aeruginosa
-
3.5.1.1 additional information enzyme activity is highest during the exponential phase of growth Rhodotorula mucilaginosa
-
3.5.1.1 additional information highest L-asparaginase production in 2% proline medium, the lowest L-asparaginase production levels is found in the presence of glutamine and urea as nitrogen sources Aspergillus tamarii
-
3.5.1.1 additional information production of high levels of L-asparaginase in solid state fermentation, using agro-wastes from three leguminous crops (bran of Cajanus cajan, Phaseolus mungo, and Glycine max) Aspergillus niger
-
3.5.1.1 additional information the lowest L-asparaginase production levels is found in the presence of glutamine and urea as nitrogen sources Aspergillus terreus
-
3.5.1.1 additional information the synthesis of L-asparaginase in Escherichia coli W and Escherichia coli K-12 is almost completely suppressed if glucose is added at a concentration of 0.5% to the growth medium. Organic acids and amino acids such as L-leucine and L-methionine enhance production of L-asparaginase. n-Dodecane at 6% increases cell concentration by 12.7% and production of L-asparaginase by 21% to give 60.8 IU/ml in the fermentation medium Escherichia coli
-
3.5.1.1 root tip
-
 Lupinus arboreus
-
3.5.1.1 seed developing Lupinus arboreus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.1 L-asparagine + H2O
-
 Mycolicibacterium phlei L-aspartate + NH3
-
 ?
3.5.1.1 L-asparagine + H2O
-
 Erwinia sp. L-aspartate + NH3
-
 ?
3.5.1.1 additional information L-asparaginase from Escherichia coli is more immuno-depressive and immunotoxic than that from Erwinia carotovora Escherichia coli ?
-
 ?
3.5.1.1 additional information L-asparaginase from Escherichia coli is more immuno-depressive and immunotoxic than that from Erwinia carotovora Pectobacterium carotovorum ?
-
 ?
3.5.1.1 additional information does not hydrolyse L-glutamine Erwinia sp. ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.1 ? x * 33000, SDS-PAGE Thermus thermophilus
3.5.1.1 hexamer
-
 Erwinia sp.
3.5.1.1 homodimer
-
 Rhodotorula toruloides

Synonyms

EC Number Synonyms Comment Organism
3.5.1.1 L-asparaginase-I
-
 Saccharomyces cerevisiae
3.5.1.1 L-asparaginase-II
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.1 30 32
-
 Staphylococcus sp.
3.5.1.1 37
-
-
 Rhodotorula toruloides

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.5.1.1 77
-
 the enzyme follows linear kinetics, even at 77°C Erwinia sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.1 50
-
 stable for 10 min Cyberlindnera jadinii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.1 6
-
-
 Cyberlindnera jadinii
3.5.1.1 6.4
-
-
 Rhodotorula toruloides
3.5.1.1 8.2
-
-
 Sphagnum fallax
3.5.1.1 8.6
-
-
 Thermus thermophilus
3.5.1.1 8.6 8.8
-
 Staphylococcus sp.
3.5.1.1 9.2
-
-
 Erwinia sp.