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Literature summary extracted from

  • Guan, F.; Zhao, D.; Pan, M.; Jiang, W.; Li, J.
    Analysis of active sites for N2 and H+ reduction on FeMo-cofactor of nitrogenase (2007), Chin. Sci. Bull., 52, 2088-2094.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.19.6.1 gene nifD, expression of wild-type and mutant enzymes Azotobacter vinelandii

Protein Variants

EC Number Protein Variants Comment Organism
1.19.6.1 alphaH195Q site-directed mutagenesis, the mutant shows an altered electron transfer substrate specificity compared to the wild-type enzyme,overview Azotobacter vinelandii
1.19.6.1 alphaQ191K site-directed mutagenesis, the mutant shows reduced electron transfer activity compared to the wild-type enzyme, it reduces only H+, not N2, overview Azotobacter vinelandii
1.19.6.1 additional information construction of Qalpha191K, Halpha195Q, nifValpha, Qalpha191K/nifValpha and Halpha195Q/nifValpha mutants and determination of their substrate specificity, the mutant strains all show reduced electron transfer, overview Azotobacter vinelandii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.19.6.1 Fe2+ as part of the Fe protein in the MoFe protein, [8Fe-7S] clusters Azotobacter vinelandii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.19.6.1 6 reduced flavodoxin + N2 + 6 H2O + 6 ATP Azotobacter vinelandii
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6 oxidized flavodoxin + 2 NH3 + 6 H+ + 6 ADP + 6 phosphate
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Organism

EC Number Organism UniProt Comment Textmining
1.19.6.1 Azotobacter vinelandii
-
-
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Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.19.6.1 additional information
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H+ and N2 reduction activities of wild-type and mutant Azotobacter vinelandii strains under 100% N2, overview Azotobacter vinelandii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.19.6.1 6 reduced flavodoxin + N2 + 6 H2O + 6 ATP
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Azotobacter vinelandii 6 oxidized flavodoxin + 2 NH3 + 6 H+ + 6 ADP + 6 phosphate
-
?
1.19.6.1 6 reduced flavodoxin + N2 + 6 H2O + 6 ATP analysis of active sites for N2 and H+ reduction on FeMo-cofactor of nitrogenase Azotobacter vinelandii 6 oxidized flavodoxin + 2 NH3 + 6 H+ + 6 ADP + 6 phosphate
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Cofactor

EC Number Cofactor Comment Organism Structure
1.19.6.1 ATP
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Azotobacter vinelandii
1.19.6.1 flavodoxin
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Azotobacter vinelandii
1.19.6.1 MoFe protein two parallel electron channels may exist between the [8Fe-7S] cluster and FeMo-cofactor Azotobacter vinelandii