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Literature summary extracted from

  • Lemaire, M.; Momparler, L.F.; Raynal, N.J.; Bernstein, M.L.; Momparler, R.L.
    Inhibition of cytidine deaminase by zebularine enhances the antineoplastic action of 5-aza-2-deoxycytidine (2009), Cancer Chemother. Pharmacol., 63, 411-416.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.4.5 functional expression of the human enzyme in murine fibroblast cell lines 3T3 NIH and 3T3-CD3-V5 cells, and in the murine lymphocytic leukemia cell line L1210 Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.4.5 zebularine inhibits the enzyme, but also DNA methylation, competitive versus 5-aza-2'-deoxycytidine, zebularine affects the antineoplastic action of 5-AZA-CdR on 3T3 cells and V5 cells transduced with CR deaminase gene, overview Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.4.5 0.0477
-
 5-aza-2'-deoxycytidine pH 7.4, 37°C Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.4.5 5-aza-2'-deoxycytidine + H2O Homo sapiens 5-AZA-CdR shows an antineoplastic action on 3T3 cells and V5 cells transduced with CR deaminase gene, pharmakokinetic analysis in mice, overview 5-aza-2'-deoxyuridine + NH3
-
 ?
3.5.4.5 cytidine + H2O Homo sapiens cytidine deaminase is a key enzyme in the catabolism of cytosine nucleoside analogues, since their deamination results in a loss of their pharmacological activity uridine + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.4.5 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.4.5 recombinant enzyme partially from 3T3 NIH and 3T3-CD3-V5 cells by ammonium sulfate fractionation Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.4.5 5-aza-2'-deoxycytidine + H2O
-
 Homo sapiens 5-aza-2'-deoxyuridine + NH3
-
 ?
3.5.4.5 5-aza-2'-deoxycytidine + H2O 5-AZA-CdR shows an antineoplastic action on 3T3 cells and V5 cells transduced with CR deaminase gene, pharmakokinetic analysis in mice, overview Homo sapiens 5-aza-2'-deoxyuridine + NH3
-
 ?
3.5.4.5 cytidine + H2O
-
 Homo sapiens uridine + NH3
-
 ?
3.5.4.5 cytidine + H2O cytidine deaminase is a key enzyme in the catabolism of cytosine nucleoside analogues, since their deamination results in a loss of their pharmacological activity Homo sapiens uridine + NH3
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.4.5 CR deaminase
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.4.5 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.4.5 7.4
-
 assay at Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.5.4.5 0.00095
-
 zebularine pH 7.4, 37°C Homo sapiens