EC Number | Cloned (Comment) | Organism |
---|---|---|
2.5.1.132 | expressed in Escherichia coli BL21-AI cells | Homo sapiens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.5.1.57 | additional information | by replacing the antifreeze-like domain of the human NeuNAc-9-P synthase with the mouse antifreeze-like domain, the chimeric protein retains almost half of the ability of the human enzyme for 2-keto-3-deoxy-D-glycero-D-galacto-nonulosonic acid-9-phosphate synthesis while the N-acetylneuraminate 9-phosphate production is reduced to less than 10%, the chimeric enzyme is less resistant to high temperatures compared to the wild type enzyme | Homo sapiens |
2.5.1.132 | additional information | replacement of the antifreeze-like domain of human N-acetylneuraminic acid phosphate synthase with the mouse antifreeze-like domain impacts both N-acetylneuraminic acid 9-phosphate synthase and 2-keto-3-deoxy-D-glycero-D-galacto-nonulosonic acid 9-phosphate synthase activities | Homo sapiens |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.5.1.57 | Mg2+ | 12.5 mM Mg2+ decrease the ability for N-acetylneuraminate 9-phosphate synthesis by almost half, while the 2-keto-3-deoxy-D-glycero-D-galacto-nonulosonic acid 9-phosphate synthetic activity drops by 20% | Homo sapiens |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.5.1.57 | Mn2+ | activity is favored by the presence of Mn2+ ions at a concentration of 12.5 mM | Homo sapiens |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.5.1.57 | 41000 | - |
2 * 41000, gel filtration | Homo sapiens |
2.5.1.57 | 43000 | - |
2 * 43000, SDS-PAGE | Homo sapiens |
2.5.1.57 | 82000 | - |
gel filtration | Homo sapiens |
2.5.1.57 | 86000 | - |
SDS-PAGE | Homo sapiens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.132 | phosphoenolpyruvate + D-mannose 6-phosphate + H2O | Homo sapiens | - |
3-deoxy-D-glycero-D-galacto-nononate 9-phosphate + phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.5.1.57 | Homo sapiens | Q9NR45 | - |
- |
2.5.1.132 | Homo sapiens | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.5.1.132 | glutathione agarose column chromatography | Homo sapiens |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.5.1.57 | 0.22 | - |
in the presence of 12.5 mM MnCl2, 8.3 mM phosphoenolpyruvate and 12.5 mM D-mannose 6-phosphate | Homo sapiens |
2.5.1.57 | 11.7 | - |
in the presence of 12.5 mM MnCl2, 8.3 mM phosphoenolpyruvate and 2.5 mM N-acyl-D-mannosamine 6-phosphate | Homo sapiens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.57 | D-mannose 6-phosphate + phosphoenolpyruvate + H2O | - |
Homo sapiens | 2-keto-3-deoxy-D-glycero-D-galacto-nonulosonic acid 9-phosphate + phosphate | - |
? | |
2.5.1.57 | N-acyl-D-mannosamine 6-phosphate + phosphoenolpyruvate + H2O | - |
Homo sapiens | N-acylneuraminate 9-phosphate + phosphate | - |
? | |
2.5.1.132 | phosphoenolpyruvate + D-mannose 6-phosphate + H2O | - |
Homo sapiens | 3-deoxy-D-glycero-D-galacto-nononate 9-phosphate + phosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.5.1.57 | homodimer | 2 * 43000, SDS-PAGE | Homo sapiens |
2.5.1.57 | homodimer | 2 * 41000, gel filtration | Homo sapiens |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.5.1.57 | N-acetylneuraminic acid phosphate synthase | - |
Homo sapiens |
2.5.1.57 | NeuNAc phosphate synthase | - |
Homo sapiens |
2.5.1.57 | NeuNAc-9-P synthase | - |
Homo sapiens |
2.5.1.132 | 2-keto-3-deoxy-D-glycero-D-galacto-nonulosonic acid 9-phosphate synthase | - |
Homo sapiens |
2.5.1.132 | N-acetylneuraminic acid phosphate synthase | also exhibits 2-keto-3-deoxy-D-glycero-D-galacto-nonulosonic acid 9-phosphate synthase activity | Homo sapiens |
2.5.1.132 | NeuNAc-9-P synthase | - |
Homo sapiens |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.5.1.57 | 37 | 65 | the enzyme loses 43% of its initial KDN-9-P synthetic activity after 10 min at 37°C or 55°C, treatment for 10 min at 65°C results in retention of 26% and 39% of N-acetylneuraminate 9-phosphate and 2-keto-3-deoxy-D-glycero-D-galacto-nonulosonic acid-9-phosphate activity respectively | Homo sapiens |