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Literature summary extracted from

  • Luo, Y.; Yuan, Z.; Luo, G.; Zhao, F.
    Expression of secreted His-tagged S-adenosylmethionine synthetase in the methylotrophic yeast Pichia pastoris and its characterization, one-step purification, and immobilization (2008), Biotechnol. Prog., 24, 214-220.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.6 expressed in Pichia pastoris Saccharomyces cerevisiae

General Stability

EC Number General Stability Organism
2.5.1.6 the Ni2+-IDA agarose-immobilized synthetase exhibits 40.4% of the free enzyme activity Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.6 AMP causes complete inactivation of the enzyme Saccharomyces cerevisiae
2.5.1.6 ATP causes complete inactivation of the enzyme Saccharomyces cerevisiae
2.5.1.6 Ba2+ 70.70% residual activity at 5 mM Saccharomyces cerevisiae
2.5.1.6 Br- 93.33% residual activity at 5 mM Saccharomyces cerevisiae
2.5.1.6 Ca2+ 86.36% residual activity at 5 mM Saccharomyces cerevisiae
2.5.1.6 CH3COO- 92.25% residual activity at 5 mM Saccharomyces cerevisiae
2.5.1.6 Cl- 85.27% residual activity at 5 mM Saccharomyces cerevisiae
2.5.1.6 Cu2+ 25.74% residual activity at 5 mM Saccharomyces cerevisiae
2.5.1.6 F- 88.84% residual activity at 5 mM Saccharomyces cerevisiae
2.5.1.6 Fe2+ 59.22% residual activity at 5 mM Saccharomyces cerevisiae
2.5.1.6 GSH causes complete inactivation of the enzyme Saccharomyces cerevisiae
2.5.1.6 I- 87.91% residual activity at 5 mM Saccharomyces cerevisiae
2.5.1.6 K+ 85.27% residual activity at 5 mM Saccharomyces cerevisiae
2.5.1.6 Li+ 81.40% residual activity at 5 mM Saccharomyces cerevisiae
2.5.1.6 Mn2+ 73.49% residual activity at 5 mM Saccharomyces cerevisiae
2.5.1.6 additional information no effect on activity at 0.1 mM Ni2+ Saccharomyces cerevisiae
2.5.1.6 Na+ 80.16% residual activity at 5 mM Saccharomyces cerevisiae
2.5.1.6 Zn2+ 22.17% residual activity at 5 mM Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.6 0.527
-
 L-methionine at pH 8.0 and 35°C Saccharomyces cerevisiae
2.5.1.6 0.631
-
 ATP at pH 8.0 and 35°C Saccharomyces cerevisiae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.6 additional information Mg2+, H2PO4-, NH4-, and NO3- have no significant effect on enzyme activity at 5 mM Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.6 Saccharomyces cerevisiae P19358
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.6 Ni2+-IDA agarose column chromatography Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.6 6.42
-
 crude extract, at 35°C Saccharomyces cerevisiae
2.5.1.6 23.83
-
 after 3.71fold purification, at 35°C Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.6 ATP + L-methionine + H2O
-
 Saccharomyces cerevisiae phosphate + diphosphate + S-adenosyl-L-methionine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.6 ATP: L-methionine S-adenosyltransferase
-
 Saccharomyces cerevisiae
2.5.1.6 S-adenosylmethionine synthetase
-
 Saccharomyces cerevisiae
2.5.1.6 SAM synthetase
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.6 35
-
-
 Saccharomyces cerevisiae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.5.1.6 30 40 enzyme activity is significantly reduced at temperatures below 30°C or above 40°C Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.6 8.5
-
-
 Saccharomyces cerevisiae

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.5.1.6 7.5 9 the activity of the enzyme is decreased above pH 9.0 and is inactive at pH below 7.5 Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
2.5.1.6 ATP
-
 Saccharomyces cerevisiae