Any feedback?
Literature summary extracted from
- Subunit dissociation and stability alteration of D-hydantoinase deleted at the terminal amino acid residue (2007), Biotechnol. Lett., 29, 303-308.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.5.2.2 | expressed in Escherichia coli BL21 (DE3) cells | Pseudomonas putida |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.5.2.2 | additional information | HYDc1 with the C-terminal Arg deletion retains 43% activity, while HYDn1 with the N-terminal Ser deletion has no activity using DL-hydantoin as substrate | Pseudomonas putida |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.5.2.2 | 54000 | - |
2 * 54000, SDS-PAGE | Pseudomonas putida |
| 3.5.2.2 | 108000 | - |
SDS-PAGE | Pseudomonas putida |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.5.2.2 | Pseudomonas putida | Q6TTR0 | YZ-26 | - |
| 3.5.2.2 | Pseudomonas putida YZ-26 | Q6TTR0 | YZ-26 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.5.2.2 | ammonium sulfate precipitation, phenyl-Sepharose column chromatography, and Sephacryl S-200 gel filtration | Pseudomonas putida |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.5.2.2 | 0.9 | - |
crude extract | Pseudomonas putida |
| 3.5.2.2 | 3.1 | - |
after 3.4fold purification | Pseudomonas putida |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.2.2 | hydantoin + H2O | - |
Pseudomonas putida | N-carbamoylglycine | - |
? |  |
| 3.5.2.2 | hydantoin + H2O | - |
Pseudomonas putida YZ-26 | N-carbamoylglycine | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.5.2.2 | dimer | 2 * 54000, SDS-PAGE | Pseudomonas putida |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.5.2.2 | D-hydantoinase | - |
Pseudomonas putida |
| 3.5.2.2 | HYD | - |
Pseudomonas putida |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.5.2.2 | 7 | 10 | - |
Pseudomonas putida |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
